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            • Primary Antibodies ›
            • CD86 Antibodies

            Invitrogen

            CD86 (B7-2) Monoclonal Antibody (GL1), FITC, eBioscience™

            98 Published Figures
            147 References
            View all (221) CD86 antibodies

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            Promo Code: CI2Z9E .

            Datasheet
            Protocols
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            Catalog # 11-0862-82
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            Cite CD86 (B7-2) Monoclonal Antibody (GL1), FITC, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (98)
            Application
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 99

            CD86 (B7-2) Antibody (11-0862-82) in Flow

            Staining of LPS-stimulated splenocytes with Anti-Mouse CD86 (B7-2) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
            View Product

            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)
            CD86 (B7-2) Antibody in Flow Cytometry (Flow)

            Product Details

            11-0862-82

            Applications
            Tested Dilution
            Publications

            Immunohistochemistry (IHC)

            -
            View 3 publications 3 publications

            Immunohistochemistry (Frozen) (IHC (F))

            -
            View 1 publication 1 publication

            Immunocytochemistry (ICC/IF)

            -
            View 2 publications 2 publications

            Flow Cytometry (Flow)

            0.125 µg/test
            View 141 publications 141 publications
            Product Specifications

            Species Reactivity

            Mouse

            Published species

            Human, Mouse, Rat

            Host/Isotype

            Rat / IgG2a, kappa

            Recommended Isotype Control

            Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            GL1

            Conjugate

            FITC
            • Unconjugated
            • Alexa Fluor 700 
            • APC 
            • APC-eFluor 780
            • Biotin 
            • Brilliant UV 395
            • Brilliant UV 737
            • Brilliant Violet 605
            • Brilliant Violet 786
            • eFluor 450
            • PE 
            • PE-Cyanine5
            • PE-Cyanine7
            • PerCP-eFluor 710
            • Super Bright 436
            • Super Bright 600
            • Super Bright 645
            • Super Bright 702
            • Request custom conjugation

            Excitation/Emission Max

            498/517 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.5 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_465148

            Product Specific Information

            Description: The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.

            Applications Reported: The GL1 antibody has been reported for use in flow cytometric analysis.

            Applications Tested: This GL1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

            Filtration: 0.2 µm post-manufacturing filtered.

            Target Information

            CD86, along with CD80, is a member of the B7 family of costimulatory molecules and plays a crucial role in T cell activation and immune response regulation. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and its expression is upregulated on B cells through various stimuli, including the BCR complex, CD40, and certain cytokine receptors. As a type I membrane protein and member of the immunoglobulin superfamily, CD86 serves as a ligand for the T cell surface proteins CD28 and CTLA-4 (CD152). The interaction between CD86 and CD28 provides a costimulatory signal essential for T cell activation during antigen presentation, while binding with CTLA-4 negatively regulates T cell activation, diminishing the immune response. This interaction is critical for T-B cell crosstalk, T cell costimulation, autoantibody production, and Th2-mediated Ig production. The kinetics of CD86 upregulation upon stimulation suggest its significant contribution during the primary phase of an immune response. CD86 and CD80 have distinct roles in T helper cell differentiation, and insufficient co-stimulation involving these molecules can induce tolerance. Alternative splicing of CD86 results in two transcript variants encoding different isoforms, with additional variants described but not fully sequenced. Dysfunction in CD86 is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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              Bioinformatics

              Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD86; early T cell costimulatory molecule-1; Early T-cell co-stimulatory molecule 1; Early T-cell costimulatory molecule 1; ETC-1; MGC34413; T-lymphocyte activation antigen CD86

              View more View less

              Gene Aliases: B7; B7-2; B7.2; B70; Cd28l2; Cd86; CLS1; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2

              View more View less

              UniProt ID: (Mouse) P42082

              View more View less

              Entrez Gene ID: (Mouse) 12524

              View more View less

              Function(s)

              receptor binding immunoglobulin receptor superfamily

              Process(es)

              toll-like receptor signaling pathway immune system process negative regulation of T cell anergy positive regulation of T cell proliferation positive regulation of activated T cell proliferation B cell activation defense response to virus cellular response to lipopolysaccharide
              It has to be done as per old AB suggested Products section.
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