Invitrogen

CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), PE, eBioscience™

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CD24 Antibody (12-0247-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD24 PE (purple histogram). Cells in the granulocyte gate were used for analysis.

CD24 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

12-0247-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 32 publications 32 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioSN3 (SN3 A5-2H10)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1548678

Product Specific Information

Description: The SN3 monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported: The SN3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioSN3 (SN3 A5-2H10) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Immune Checkpoint Profiling in Humanized Breast Cancer Mice Revealed Cell-Specific LAG-3/PD-1/TIM-3 Co-Expression and Elevated PD-1/TIM-3 Secretion.

Cancers

Bruss C,Kellner K,Albert V,Hutchinson JA,Seitz S,Ortmann O,Brockhoff G,Wege AK

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Thu May 04 00:00:00 EDT 2023

miR‑122/SENP1 axis confers stemness and chemoresistance to liver cancer through Wnt/β‑catenin signaling.

Oncology letters

Dai J,Hao Y,Chen X,Yu Q,Wang B

12-0247-42 was used in Flow cytometry/Cell sorting to identify the molecular targets of miR-122 whilst uncovering the mechanism underlying chemoresistance and stemness of HepG2 cells in liver cancer.

Fri Sep 01 00:00:00 EDT 2023

miR‑122/SENP1 axis confers stemness and chemoresistance to liver cancer through Wnt/β‑catenin signaling.

Oncology letters

Dai J,Hao Y,Chen X,Yu Q,Wang B

12-0247-42 was used in Flow cytometry/Cell sorting to identify the molecular targets of miR-122 whilst uncovering the mechanism underlying chemoresistance and stemness of HepG2 cells in liver cancer.

Fri Sep 01 00:00:00 EDT 2023

Naringin protects human nucleus pulposus cells against TNF-α-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation.

Oxidative medicine and cellular longevity

Chen R,Gao S,Guan H,Zhang X,Gao Y,Su Y,Song Y,Jiang Y,Li N

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Thu Mar 24 00:00:00 EDT 2022

Elevated transcription and glycosylation of B3GNT5 promotes breast cancer aggressiveness.

Journal of experimental & clinical cancer research : CR

Miao Z,Cao Q,Liao R,Chen X,Li X,Bai L,Ma C,Deng X,Dai Z,Li J,Dong C

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Sat May 07 00:00:00 EDT 2022

Relationship of shear wave elastography anisotropy with tumor stem cells and epithelial-mesenchymal transition in breast cancer.

BMC medical imaging

Leng X,Japaer R,Zhang H,Yeerlan M,Ma F,Ding J

12-0247-42 was used in Immunohistochemistry to examine the feasibility of shear wave elastography (SWE) anisotropy in assessing the prognosis of breast cancer.

Wed Nov 17 00:00:00 EST 2021

Relationship of shear wave elastography anisotropy with tumor stem cells and epithelial-mesenchymal transition in breast cancer.

BMC medical imaging

Leng X,Japaer R,Zhang H,Yeerlan M,Ma F,Ding J

12-0247-42 was used in Immunohistochemistry to examine the feasibility of shear wave elastography (SWE) anisotropy in assessing the prognosis of breast cancer.

Wed Nov 17 00:00:00 EST 2021

Alterations in T and B cell function persist in convalescent COVID-19 patients.

Med (New York, N.Y.)

Shuwa HA,Shaw TN,Knight SB,Wemyss K,McClure FA,Pearmain L,Prise I,Jagger C,Morgan DJ,Khan S,Brand O,Mann ER,Ustianowski A,Bakerly ND,Dark P,Brightling CE,Brij S,Felton T,Simpson A,Grainger JR,Hussell T,Konkel JE,Menon M

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Fri Jun 11 00:00:00 EDT 2021

A CD10-OGP Membrane Peptolytic Signaling Axis in Fibroblasts Regulates Lipid Metabolism of Cancer Stem Cells via SCD1.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Yu S,Lu Y,Su A,Chen J,Li J,Zhou B,Liu X,Xia Q,Li Y,Li J,Huang M,Ye Y,Zhao Q,Jiang S,Yan X,Wang X,Di C,Pan J,Su S

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

DNAJB6, a Key Factor in Neuronal Sensitivity to Amyloidogenesis.

Molecular cell

Thiruvalluvan A,de Mattos EP,Brunsting JF,Bakels R,Serlidaki D,Barazzuol L,Conforti P,Fatima A,Koyuncu S,Cattaneo E,Vilchez D,Bergink S,Boddeke EHWG,Copray S,Kampinga HH

12-0247 was used in Flow cytometry/Cell sorting to show that stem cells are intrinsically protected against amyloidogenesis and protein aggregates are dominantly prese in vivo.nt in neurons due to loss of DNAJB6 expression upon differentiation.

Thu Apr 16 00:00:00 EDT 2020

IGF2BP2 regulates DANCR by serving as an N6-methyladenosine reader.

Cell death and differentiation

Hu X,Peng WX,Zhou H,Jiang J,Zhou X,Huang D,Mo YY,Yang L

12-0247-42 was used in Flow Cytometry to promote cancer stemness-like properties and pancreatic cancer pathogenesis.

Mon Jun 01 00:00:00 EDT 2020

IGF2BP2 regulates DANCR by serving as an N6-methyladenosine reader.

Cell death and differentiation

Hu X,Peng WX,Zhou H,Jiang J,Zhou X,Huang D,Mo YY,Yang L

12-0247-42 was used in Flow Cytometry to promote cancer stemness-like properties and pancreatic cancer pathogenesis.

Mon Jun 01 00:00:00 EDT 2020

Changes in Tumor Stem Cell Markers and Epithelial-Mesenchymal Transition Markers in Nonluminal Breast Cancer after Neoadjuvant Chemotherapy and Their Correlation with Contrast-Enhanced Ultrasound.

BioMed research international

Leng X,Huang G,Zhang L,Ding J,Ma F

12-0247 was used in Flow cytometry/Cell sorting to investigate the changes in CSC markers and EMT markers after NAC in nonluminal breast cancer and their correlation with contrast-enhanced ultrasound (CEUS) features and chemotherapy efficacy.

Fri May 07 00:00:00 EDT 2021

Chemotherapeutic Stress Influences Epithelial-Mesenchymal Transition and Stemness in Cancer Stem Cells of Triple-Negative Breast Cancer.

International journal of molecular sciences

Li X,Strietz J,Bleilevens A,Stickeler E,Maurer J

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Wed Jan 08 00:00:00 EST 2020

Obesity-Altered Adipose Stem Cells Promote Radiation Resistance of Estrogen Receptor Positive Breast Cancer through Paracrine Signaling.

International journal of molecular sciences

Sabol RA,Villela VA,Denys A,Freeman BT,Hartono AB,Wise RM,Harrison MAA,Sandler MB,Hossain F,Miele L,Bunnell BA

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Wed Apr 15 00:00:00 EDT 2020

SOX9/FXYD3/Src Axis Is Critical for ER⁺ Breast Cancer Stem Cell Function.

Molecular cancer research : MCR

Xue Y,Lai L,Lian W,Tu X,Zhou J,Dong P,Su D,Wang X,Cao X,Chen Y,Wang Q

12-0247 was used in Flow cytometry/Cell sorting to show that an estrogen-inducible gene FXYD3, whose expression is significantly upregulated in ER+ breast CSCs, is a critical player for regulating ER+ breast CSC function.

Tue Jan 01 00:00:00 EST 2019

HMGB3 silence inhibits breast cancer cell proliferation and tumor growth by interacting with hypoxia-inducible factor 1α.

Cancer management and research

Gu J,Xu T,Huang QH,Zhang CM,Chen HY

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

BRCA1 regulates the cancer stem cell fate of breast cancer cells in the context of hypoxia and histone deacetylase inhibitors.

Scientific reports

Kim H,Lin Q,Yun Z

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Thu Jul 04 00:00:00 EDT 2019

The hypoxic tumor microenvironment in vivo selects the cancer stem cell fate of breast cancer cells.

Breast cancer research : BCR

Kim H,Lin Q,Glazer PM,Yun Z

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Tue Mar 06 00:00:00 EST 2018

Glutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.

Theranostics

Cao Y,Lin SH,Wang Y,Chin YE,Kang L,Mi J

12-0247 was used in Flow cytometry/Cell sorting to show that GPT2 promotes tumorigenesis and stemness of breast cancer cells by activating the Shh signalling.

Mon Apr 30 00:00:00 EDT 2018

HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells.

Molecular cancer

Shah M,Cardenas R,Wang B,Persson J,Mongan NP,Grabowska A,Allegrucci C

12-0247 was used in Flow cytometry/Cell sorting to to study the role of HOXC8 regulation in breast cancer stem cells.

Thu Feb 16 00:00:00 EST 2017

Stage-specific embryonic antigen-3 (SSEA-3) and β3GalT5 are cancer specific and significant markers for breast cancer stem cells.

Proceedings of the National Academy of Sciences of the United States of America

Cheung SK,Chuang PK,Huang HW,Hwang-Verslues WW,Cho CH,Yang WB,Shen CN,Hsiao M,Hsu TL,Chang CF,Wong CH

12-0247 was used in Flow cytometry/Cell sorting to investigate significant markers for breast cancer stem cells, such as (SSEA-3) and β3GalT5.

Tue Jan 26 00:00:00 EST 2016

CD16⁺ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.

Frontiers in immunology

Zhu H,Hu F,Sun X,Zhang X,Zhu L,Liu X,Li X,Xu L,Shi L,Gan Y,Su Y

12-0247 was used in Flow cytometry/Cell sorting to demonstrate that CD16+monocytes were enriched and played a critical role in driving the pathogenic T- and B-cell responses in patients with systemic lupus erythematosus.

Wed Nov 20 00:00:00 EST 2019

The Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states.

BMC biology

Toneff MJ,Sreekumar A,Tinnirello A,Hollander PD,Habib S,Li S,Ellis MJ,Xin L,Mani SA,Rosen JM

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Fri Jun 17 00:00:00 EDT 2016

Llgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.

Oncotarget

Greenwood E,Maisel S,Ebertz D,Russ A,Pandey R,Schroeder J

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Tue Sep 20 00:00:00 EDT 2016

B cells from African American lupus patients exhibit an activated phenotype.

JCI insight

Menard LC,Habte S,Gonsiorek W,Lee D,Banas D,Holloway DA,Manjarrez-Orduno N,Cunningham M,Stetsko D,Casano F,Kansal S,Davis PM,Carman J,Zhang CK,Abidi F,Furie R,Nadler SG,Suchard SJ

12-0247 was used in Flow cytometry/Cell sorting to investigate immunological differences between African American and European American patients with systemic lupus erythematosus, showing that B cells from African American patients exhibit an activated phenotype.

Thu Jun 16 00:00:00 EDT 2016

TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis.

Oncogene

Cyr AR,Kulak MV,Park JM,Bogachek MV,Spanheimer PM,Woodfield GW,White-Baer LS,O'Malley YQ,Sugg SL,Olivier AK,Zhang W,Domann FE,Weigel RJ

12-0247 was used in Flow cytometry/Cell sorting to suggest that TFAP2C has an important role in regulated luminal-specific genes and may be a viable therapeutic target in breast cancer.

Thu Jan 22 00:00:00 EST 2015

Special AT-rich sequence-binding protein-1 participates in the maintenance of breast cancer stem cells through regulation of the Notch signaling pathway and expression of Snail1 and Twist1.

Molecular medicine reports

Sun Z,Zhang C,Zou X,Jiang G,Xu Z,Li W,Xie H

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Fri May 01 00:00:00 EDT 2015

Immunologic human renal allograft injury associates with an altered IL-10/TNF-α expression ratio in regulatory B cells.

Journal of the American Society of Nephrology : JASN

Cherukuri A,Rothstein DM,Clark B,Carter CR,Davison A,Hernandez-Fuentes M,Hewitt E,Salama AD,Baker RJ

12-0247 was used in Flow cytometry/Cell sorting to explore proinflammatory cytokine expression in B-cell subsets.

Tue Jul 01 00:00:00 EDT 2014

NDY1/KDM2B functions as a master regulator of polycomb complexes and controls self-renewal of breast cancer stem cells.

Cancer research

Kottakis F,Foltopoulou P,Sanidas I,Keller P,Wronski A,Dake BT,Ezell SA,Shen Z,Naber SP,Hinds PW,McNiel E,Kuperwasser C,Tsichlis PN

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Tue Jul 15 00:00:00 EDT 2014

Expression of intercellular adhesion molecule 1 by hepatocellular carcinoma stem cells and circulating tumor cells.

Gastroenterology

Liu S,Li N,Yu X,Xiao X,Cheng K,Hu J,Wang J,Zhang D,Cheng S,Liu S

12-0247 was used in Flow cytometry/Cell sorting to demonstrate that the stem cell factor Nanog regulates expression of ICAM-1 in hepatocellular carcinoma cells.

Wed May 01 00:00:00 EDT 2013

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

CXC chemokine receptor 4 is essential for maintenance of renal cell carcinoma-initiating cells and predicts metastasis.

Stem cells (Dayton, Ohio)

Gassenmaier M,Chen D,Buchner A,Henkel L,Schiemann M,Mack B,Schendel DJ,Zimmermann W,Pohla H

12-0247 was used in Flow cytometry/Cell sorting to study the expression of CXC chemokine receptor 4 in a subpopulation of tumour-initiating cells in renal cell carcinoma cell lines and study its role in their maintenance.

Thu Aug 01 00:00:00 EDT 2013

Increased invasion and tumorigenicity capacity of CD44+/CD24- breast cancer MCF7 cells in vitro and in nude mice.

Cancer cell international

Yan W,Chen Y,Yao Y,Zhang H,Wang T

12-0247 was used in Flow cytometry/Cell sorting to identify CD cell surface markers as breast cancer stem cell markers.

Mon Jun 24 00:00:00 EDT 2013

Combined flow cytometric analysis of surface and intracellular antigens reveals surface molecule markers of human neuropoiesis.

PloS one

Turaç G,Hindley CJ,Thomas R,Davis JA,Deleidi M,Gasser T,Karaöz E,Pruszak J

12-0247 was used in Flow cytometry/Cell sorting to develop a validated protocol enabling the co-detection and co-expression of surface molecules on neural cell populations.

Tue Feb 04 00:00:00 EST 2014

Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways.

Breast cancer research : BCR

Iwanaga R,Wang CA,Micalizzi DS,Harrell JC,Jedlicka P,Sartorius CA,Kabos P,Farabaugh SM,Bradford AP,Ford HL

Published figure using CD24 monoclonal antibody (Product # 12-0247-42) in Flow Cytometry

Thu Jul 05 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); FLJ22950; FLJ43543; MGC75043; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen

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Gene Aliases: CD24; CD24A

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UniProt ID: (Human) P25063

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Entrez Gene ID: (Human) 100133941

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Function(s)

signal transducer activity protein binding protein kinase binding protein tyrosine kinase activator activity

Process(es)

response to hypoxia cell activation regulation of cytokine-mediated signaling pathway response to molecule of bacterial origin immune response-regulating cell surface receptor signaling pathway positive regulation of cytosolic calcium ion concentration Wnt signaling pathway single organismal cell-cell adhesion cell migration regulation of epithelial cell differentiation T cell costimulation B cell receptor transport into membrane raft chemokine receptor transport out of membrane raft negative regulation of transforming growth factor beta3 production positive regulation of activated T cell proliferation regulation of phosphorylation cholesterol homeostasis positive regulation of MAP kinase activity regulation of MAPK cascade response to estrogen respiratory burst positive regulation of protein tyrosine kinase activity glomerular visceral epithelial cell differentiation glomerular parietal epithelial cell differentiation intrinsic apoptotic signaling pathway positive regulation of nephron tubule epithelial cell differentiation

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CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), PE, eBioscience™

CD24 Antibody (12-0247-42) in Flow

Product Details

12-0247-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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