Invitrogen

CD338 (ABCG2) Monoclonal Antibody (5D3), PE, eBioscience™

CD338 (ABCG2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD338 (ABCG2) Antibody (12-8888-42) in Flow

Staining of human CD338-transfected cells with Mouse IgG2b K Isotype Control PE (Product # 12-4732-81) (open histogram) or Anti-Human CD338 (ABCG2) PE (filled histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

12-8888-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Human

Published species

Dog, Human, Mouse, Pig

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

5D3

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10854716

Product Specific Information

Description: The 5D3 monoclonal antibody reacts with the extracellular portion of the human ABCG2 protein, also known as Bcrp1 and MXR. The ABCG2 gene, a member of the multi-drug resistance (MDR) family, is highly expressed on primitive 'side-population' (SP) stem cells, which are defined by the efflux of fluorescent dyes such as Rhodamine 123 and Hoechest 33342. In the bone marrow, about 0.05% of cells display the low fluorescence and are highly enriched for repopulating cells. The SP cells, which express low or undetectable levels of CD34, have been identified in multiple species. In addition, expression of ABCG2 appears to be highly conserved.

Applications Reported:The 5D3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: Has been pre-titrated and tested by flow cytometric analysis of ABCG2 transfected cells and peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ABCG2, is also known as placenta-specific ABC transporter and breast cancer resistance protein (BCRP1). ABCG2 confers resistance for a variety of chemotherapeutic agents, including anthracyclines, mitoxantrone, bisantrene and topotecan. Under normal conditions, ABCG2 may serve a protective function by removing toxins from the cell, and plays an important role in regulating stem cell differentiation. ABCG2 is responsible for the side population (SP) phenotype and is widely expressed in a large variety of stem cells, making it an important stem cell marker. ABCG2 may have N-linked glycosylation and may dimerize in vivo. ABCG2 is abundantly expressed in placenta, liver, intestine and stem cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Engineering Escherichia coli for diagnosis and management of hyperuricemia.

Frontiers in bioengineering and biotechnology

Gencer G,Mancuso C,Chua KJ,Ling H,Costello CM,Chang MW,March JC

12-8888-42 was used in Flow cytometry/Cell sorting to show that reprogramming E. coli holds promise as a valid alternative synthetic biology therapy to monitor and maintain healthy serum uric acid levels.

Sat Jun 10 00:00:00 EDT 2023

CBP-mediated Wnt3a/β-catenin signaling promotes cervical oncogenesis initiated by Piwil2.

Neoplasia (New York, N.Y.)

Feng D,Yan K,Liang H,Liang J,Wang W,Yu H,Zhou Y,Zhao W,Dong Z,Ling B

12-8888 was used in Flow cytometry/Cell sorting to determine that canonical Wnt signaling was aberrantly activated in HaCaT cells transfected with lentivirus expressing Piwil2 and in cervical lesion specimens of low-grade squamous intraepithelial lesion, high-grade squamous intraepithelial lesion, and invasive carcinoma.

Fri Jan 01 00:00:00 EST 2021

CBP-mediated Wnt3a/β-catenin signaling promotes cervical oncogenesis initiated by Piwil2.

Neoplasia (New York, N.Y.)

Feng D,Yan K,Liang H,Liang J,Wang W,Yu H,Zhou Y,Zhao W,Dong Z,Ling B

Fri Jan 01 00:00:00 EST 2021

Tariquidar Is an Inhibitor and Not a Substrate of Human and Mouse P-glycoprotein.

Drug metabolism and disposition: the biological fate of chemicals

Weidner LD,Fung KL,Kannan P,Moen JK,Kumar JS,Mulder J,Innis RB,Gottesman MM,Hall MD

12-8888 was used in Flow cytometry/Cell sorting to conclude that TQR is a potent inhibitor of human and mouse P-gp and shows no signs of being a substrate.

Mon Feb 01 00:00:00 EST 2016

Tariquidar Is an Inhibitor and Not a Substrate of Human and Mouse P-glycoprotein.

Drug metabolism and disposition: the biological fate of chemicals

Weidner LD,Fung KL,Kannan P,Moen JK,Kumar JS,Mulder J,Innis RB,Gottesman MM,Hall MD

12-8888 was used in Flow cytometry/Cell sorting to conclude that TQR is a potent inhibitor of human and mouse P-gp and shows no signs of being a substrate.

Mon Feb 01 00:00:00 EST 2016

Using the BacMam Baculovirus System to Study Expression and Function of Recombinant Efflux Drug Transporters in Polarized Epithelial Cell Monolayers.

Drug metabolism and disposition: the biological fate of chemicals

Fung KL,Kapoor K,Pixley JN,Talbert DJ,Kwit AD,Ambudkar SV,Gottesman MM

12-8888-42 was used in Flow cytometry/Cell sorting to evaluate the efficacy of the BacMam baculovirus system to transduce cells grown in monolayers.

Mon Feb 01 00:00:00 EST 2016

Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis.

Oncotarget

Feng D,Yan K,Zhou Y,Liang H,Liang J,Zhao W,Dong Z,Ling B

Published figure using CD338 (ABCG2) monoclonal antibody (Product # 12-8888-42) in Flow Cytometry

Tue Oct 04 00:00:00 EDT 2016

Canine osteosarcoma cells exhibit resistance to aurora kinase inhibitors.

Veterinary and comparative oncology

Cannon CM,Pozniak J,Scott MC,Ito D,Gorden BH,Graef AJ,Modiano JF

12-8888 was used in Flow cytometry/Cell sorting to investigate the effect of Aurora kinase inhibitors AZD1152 and VX680 on canine osteosarcoma cells.

Sun Mar 01 00:00:00 EST 2015

A double blinded, placebo-controlled pilot study to examine reduction of CD34 ⁺/CD117 ⁺/CD133 ⁺ lymphoma progenitor cells and duration of remission induced by neoadjuvant valspodar in dogs with large B-cell lymphoma.

F1000Research

Ito D,Childress M,Mason N,Winter A,O'Brien T,Henson M,Borgatti A,Lewellen M,Krick E,Stewart J,Lahrman S,Rajwa B,Scott MC,Seelig D,Koopmeiners J,Ruetz S,Modiano J

12-8888 was used in Flow cytometry/Cell sorting to examine whether ABCB1 would reduce lymphoma progenitor cells, and extend the length of remission in dogs with therapy naive large B-cell lymphoma.

Tue Sep 14 00:00:00 EDT 2021

ABCG2pos lung mesenchymal stem cells are a novel pericyte subpopulation that contributes to fibrotic remodeling.

American journal of physiology. Cell physiology

Marriott S,Baskir RS,Gaskill C,Menon S,Carrier EJ,Williams J,Talati M,Helm K,Alford CE,Kropski JA,Loyd J,Wheeler L,Johnson J,Austin E,Nozik-Grayck E,Meyrick B,West JD,Klemm DJ,Majka SM

12-8888 was used in Flow cytometry/Cell sorting to highlight ABCG2(pos) lung MSC as a novel cell population that contributes to detrimental myofibroblast-mediated remodeling during pulmonary fibrosis.

Wed Oct 15 00:00:00 EDT 2014

Lysosomal drug sequestration as a mechanism of drug resistance in vascular sarcoma cells marked by high CSF-1R expression.

Vascular cell

Gorden BH,Saha J,Khammanivong A,Schwartz GK,Dickerson EB

12-8888 was used in Flow cytometry/Cell sorting to identify drug sequestration within cellular lysosomes as a drug resistance mechanism in vascular sarcomas marked by high CSF-1R expression.

Wed Oct 08 00:00:00 EDT 2014

Targeting the ABCG2-overexpressing multidrug resistant (MDR) cancer cells by PPARγ agonists.

British journal of pharmacology

To KK,Tomlinson B

12-8888 was used in Flow cytometry/Cell sorting to evaluate the potential circumvention of ABCG2-mediated multidrug resistance by commonly prescribed PPARγ agonists.

Fri Nov 01 00:00:00 EDT 2013

Ethyl 2-amino-6-(3,5-dimethoxyphenyl)-4-(2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate (CXL017): a novel scaffold that resensitizes multidrug resistant leukemia cells to chemotherapy.

ACS chemical biology

Das SG,Hermanson DL,Bleeker N,Lowman X,Li Y,Kelekar A,Xing C

12-8888 was used in Flow cytometry/Cell sorting to present new evidence for treatment of MDR cancers and offer mechanistic insights toward its preferential targeting of drug-resistant cancer.

Fri Feb 15 00:00:00 EST 2013

Transcription factors Sp1 and Sp3 regulate expression of human ABCG2 gene and chemoresistance phenotype.

Molecules and cells

Yang WJ,Song MJ,Park EY,Lee JJ,Park JH,Park K,Park JH,Kim HP

12-8888 was used in Flow cytometry/Cell sorting to demonstrate that Sp1 and Sp3 transcription factors may play an important role in maintaining the side population phenotype of lung cancer cells.

Tue Oct 01 00:00:00 EDT 2013

Human prostate side population cells demonstrate stem cell properties in recombination with urogenital sinus mesenchyme.

PloS one

Foster BA,Gangavarapu KJ,Mathew G,Azabdaftari G,Morrison CD,Miller A,Huss WJ

12-8888 was used in Immunohistochemistry to show that the ABCG2 expressing side population from freshly digested prostate tissue is enriched for prostate stem cells.

Fri Jul 19 00:00:00 EDT 2013

New use for an old drug: inhibiting ABCG2 with sorafenib.

Molecular cancer therapeutics

Wei Y,Ma Y,Zhao Q,Ren Z,Li Y,Hou T,Peng H

12-8888 was used in Flow cytometry/Cell sorting to rediscover sorafenib as a potent inhibitor of ABCG2 in vitro.

Wed Aug 01 00:00:00 EDT 2012

Surface antigenic profiling of stem cells from human omentum fat in comparison with subcutaneous fat and bone marrow.

Cytotechnology

Dhanasekaran M,Indumathi S,Kanmani A,Poojitha R,Revathy KM,Rajkumar JS,Sudarsanam D

12-8888 was used in Flow cytometry/Cell sorting to investigate surface antigenic profiling of stem cells from human omentum fat, compared with subcutaneous fat and bone marrow..

Wed Feb 01 00:00:00 EST 2012

Adverse Cell Culture Conditions Mimicking the Tumor Microenvironment Upregulate ABCG2 to Mediate Multidrug Resistance and a More Malignant Phenotype.

ISRN oncology

Cheng GM,To KK

12-8888 was used in Flow cytometry/Cell sorting to investigate the upregulation of ABCG2 by hypoxia, glucose deprivation and acidosis within the tumor microenvironment.

Thu Aug 23 00:00:00 EDT 2012

Identification of proline residues in or near the transmembrane helices of the human breast cancer resistance protein (BCRP/ABCG2) that are important for transport activity and substrate specificity.

Biochemistry

Ni Z,Bikadi Z,Shuster DL,Zhao C,Rosenberg MF,Mao Q

Published figure using CD338 (ABCG2) monoclonal antibody (Product # 12-8888-42) in Flow Cytometry

Tue Sep 20 00:00:00 EDT 2011

Structure and function of ABCG2-rich extracellular vesicles mediating multidrug resistance.

PloS one

Goler-Baron V,Assaraf YG

12-8888 was used in Flow cytometry/Cell sorting to propose a composite model for the structure and function of MDR pump-rich EVs in cancer cells and their ability to confer multiple anticancer drug resistance.

Mon Jan 24 00:00:00 EST 2011

Transmembrane helices 1 and 6 of the human breast cancer resistance protein (BCRP/ABCG2): identification of polar residues important for drug transport.

American journal of physiology. Cell physiology

Ni Z,Bikadi Z,Cai X,Rosenberg MF,Mao Q

12-8888 was used in Flow cytometry/Cell sorting to demonstrate the functional importance of several polar residues in breast cancer resistance protein activity.

Mon Nov 01 00:00:00 EDT 2010

Role of basic residues within or near the predicted transmembrane helix 2 of the human breast cancer resistance protein in drug transport.

The Journal of pharmacology and experimental therapeutics

Cai X,Bikadi Z,Ni Z,Lee EW,Wang H,Rosenberg MF,Mao Q

12-8888 was used in Flow cytometry/Cell sorting to investigate the role of five basic residues, within or near the transmembrane region of breast cancer resistance protein, in transport activity.

Tue Jun 01 00:00:00 EDT 2010

Structural determinants of imidazoacridinones facilitating antitumor activity are crucial for substrate recognition by ABCG2.

Molecular pharmacology

Bram EE,Adar Y,Mesika N,Sabisz M,Skladanowski A,Assaraf YG

12-8888 was used in Immunofluorescence to demonstrate that the imidazoacridinone (IA) determinants mediating cytotoxicity also facilitate ABCG2-dependent drug efflux and IA resistance.

Fri May 01 00:00:00 EDT 2009

Becatecarin (rebeccamycin analog, NSC 655649) is a transport substrate and induces expression of the ATP-binding cassette transporter, ABCG2, in lung carcinoma cells.

Cancer chemotherapy and pharmacology

Robey RW,Obrzut T,Shukla S,Polgar O,Macalou S,Bahr JC,Di Pietro A,Ambudkar SV,Bates SE

12-8888 was used in Flow cytometry/Cell sorting to suggest that becatecarin is transported by ABCG2 and is able to induce ABCG2 expression in cancer cells.

Sat Aug 01 00:00:00 EDT 2009

Arginine 383 is a crucial residue in ABCG2 biogenesis.

Biochimica et biophysica acta

Polgar O,Ediriwickrema LS,Robey RW,Sharma A,Hegde RS,Li Y,Xia D,Ward Y,Dean M,Ozvegy-Laczka C,Sarkadi B,Bates SE

12-8888 was used in Flow cytometry/Cell sorting to conclude that arginine 383 is a crucial residue for ABCG2 biogenesis, where even the most conservative mutations have a large impact.

Wed Jul 01 00:00:00 EDT 2009

Identification of cancer stem cell-like side population cells in human nasopharyngeal carcinoma cell line.

Cancer research

Wang J,Guo LP,Chen LZ,Zeng YX,Lu SH

12-8888 was used in Flow cytometry/Cell sorting to investigate the prevalence of side population cells and explore stem cell characteristics in a human nasopharyngeal carcinoma cell line.

Sun Apr 15 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: ABC transporter; ATP-binding cassette sub-family G member 2; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group); Breast cancer resistance protein; Broad substrate specificity ATP-binding cassette transporter ABCG2; CD338; CDw338; Mitoxantrone resistance-associated protein; multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2; placenta specific MDR protein; Placenta-specific ATP-binding cassette transporter; Urate exporter

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Gene Aliases: ABC15; ABCG2; ABCP; BCRP; BCRP1; BMDP; CD338; CDw338; EST157481; GOUT1; MRX; MXR; MXR-1; MXR1; UAQTL1

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UniProt ID: (Human) Q9UNQ0

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Entrez Gene ID: (Human) 9429

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Function(s)

transporter activity protein binding ATP binding xenobiotic-transporting ATPase activity heme transporter activity ATPase activity, coupled to transmembrane movement of substances protein homodimerization activity ATP-binding cassette (ABC) transporter primary active transporter transporter

Process(es)

transport drug transmembrane transport cellular iron ion homeostasis response to iron ion heme transport response to drug xenobiotic transport urate metabolic process drug export response to folic acid embryonic process involved in female pregnancy cellular response to dexamethasone stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD338 (ABCG2) Monoclonal Antibody (5D3), PE, eBioscience™

CD338 (ABCG2) Antibody (12-8888-42) in Flow

Product Details

12-8888-42

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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