Invitrogen

CD283 (TLR3) Monoclonal Antibody (TLR3.7), PE, eBioscience™

CD283 (TLR3) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 4

CD283 (TLR3) Antibody (12-9039-82) in Flow

Staining of permeabilized (left) and non-permeabilized (right) A549 cells with 0.5 µg of Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (open histogram) or 0.5 µg of Anti-Human CD283 (TLR3) PE (filled histogram). Total cells were used for analysis.

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Product Details

12-9039-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 23 publications 23 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Pig

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TLR3.7

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_823150

Product Specific Information

Description: The TLR3.7 monoclonal antibody reacts with human Toll-like receptor 3 (TLR3). To date, at least twelve members of the Toll-like receptor family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. In the innate immune response, TLRs recognize molecular patterns associated with microbial pathogens and induce antimicrobial activity. TLR3 recognizes double-stranded (ds)RNA, induces the activation of NF-kappaB through MyD88-dependent and -independent pathways, and the production of type I interferons (IFNs). TLR3.7 suppressed poly(I):poly(C)-mediated IFN-beta production by human fibroblasts naturally expressing TLR3 on their surface.

Applications Reported: The TLR3.7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TLR3.7 antibody has been tested by intracellular staining and flow cytometric analysis of A549 and MRC5 cell lines to detect the very low level of this antigen on the surface. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TLR3 (Toll-like receptor 3, CD283) is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from drosophila to humans and share structural and functional similarities. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR3 is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. TLR3 recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. Further, TLR3 may play a role in host defense against viruses, and the use of alternative polyadenylation sites to generate different length transcripts has been noted for the TLR3 gene. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. TLR3 is the only human TLR which does not utilize MyD88 as an adaptor molecule instead utilizing TRIF in a signaling path which results in downstream activation of IRF-3 and NF-kB. TLR3 activation leads not only to Type I interferon induction but also to other inflammatory cytokines which result in DC maturation. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.

The Journal of international medical research

Li X,Zhang B,Wang H,Zhao X,Zhang Z,Ding G,Wei F

12-9039 was used in Flow cytometry/Cell sorting to investigate the effects of age on immunophenotype, proliferation, activation, and cytokine secretion capacities of PBMCs following co-culture with hPDLSCs.

Wed Jul 01 00:00:00 EDT 2020

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.

The Journal of international medical research

Li X,Zhang B,Wang H,Zhao X,Zhang Z,Ding G,Wei F

Wed Jul 01 00:00:00 EDT 2020

The expression profile of virus-recognizing toll-like receptors in natural killer cells of Cypriot multiple sclerosis patients.

BMC research notes

Deeba E,Lambrianides A,Pantzaris M,Krashias G,Christodoulou C

12-9039-82 was used in Flow Cytometry to study toll-like receptor (TLR) expression in the natural killer cells of patients with multiple sclerosis.

Tue Sep 29 00:00:00 EDT 2020

Hepatitis C virus infection induces autocrine interferon signaling by human liver endothelial cells and release of exosomes, which inhibits viral replication.

Gastroenterology

Giugliano S,Kriss M,Golden-Mason L,Dobrinskikh E,Stone AE,Soto-Gutierrez A,Mitchell A,Khetani SR,Yamane D,Stoddard M,Li H,Shaw GM,Edwards MG,Lemon SM,Gale M,Shah VH,Rosen HR

12-9039 was used in Flow cytometry/Cell sorting to demonstrate that cultured human liver sinusoidal endothelial cells produce factors that mediate immunity against hepatitis C virus during infection.

Sun Feb 01 00:00:00 EST 2015

Hepatitis C Virus Sensing by Human Trophoblasts Induces Innate Immune Responses and Recruitment of Maternal NK Cells: Potential Implications for Limiting Vertical Transmission.

Journal of immunology (Baltimore, Md. : 1950)

Giugliano S,Petroff MG,Warren BD,Jasti S,Linscheid C,Ward A,Kramer A,Dobrinskikh E,Sheiko MA,Gale M,Golden-Mason L,Winn VD,Rosen HR

12-9039 was used in Flow cytometry/Cell sorting to demonstrate that HCV-RNA sensing by human trophoblasts induces recruitment and activation of innate immune cells.

Thu Oct 15 00:00:00 EDT 2015

A potential mode of action for Anakinra in patients with arthrofibrosis following total knee arthroplasty.

Scientific reports

Dixon D,Coates J,del Carpio Pons A,Horabin J,Walker A,Abdul N,Kalson NS,Brewster NT,Weir DJ,Deehan DJ,Mann DA,Borthwick LA

12-9039 was used in Flow cytometry/Cell sorting to propose a potential mechanism for the reported beneficial impact of Anakinra on patients with arthrofibrosis.

Tue Nov 10 00:00:00 EST 2015

A potential mode of action for Anakinra in patients with arthrofibrosis following total knee arthroplasty.

Scientific reports

Dixon D,Coates J,del Carpio Pons A,Horabin J,Walker A,Abdul N,Kalson NS,Brewster NT,Weir DJ,Deehan DJ,Mann DA,Borthwick LA

12-9039 was used in Flow cytometry/Cell sorting to propose a potential mechanism for the reported beneficial impact of Anakinra on patients with arthrofibrosis.

Tue Nov 10 00:00:00 EST 2015

Syndrome of selective IgM deficiency with severe T cell deficiency associated with disseminated cutaneous mycobacterium avium intracellulaire infection.

American journal of clinical and experimental immunology

Gharib A,Louis AG,Agrawal S,Gupta S

12-9039 was used in Flow cytometry/Cell sorting to analyse the selective IgM deficiency and T cell lymphocytopenia associated with disseminated cutaneous Mycobacterium avium intracellulaire infection.

Mon Nov 09 00:00:00 EST 2015

IL-29 enhances Toll-like receptor-mediated IL-6 and IL-8 production by the synovial fibroblasts from rheumatoid arthritis patients.

Arthritis research & therapy

Xu L,Feng X,Tan W,Gu W,Guo D,Zhang M,Wang F

12-9039 was used in Flow cytometry/Cell sorting to investigate the effect of IL-29 on cytokine production in synovial fibroblasts in arthritis patients.

Tue Oct 29 00:00:00 EDT 2013

Identification of lineage relationships and novel markers of blood and skin human dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Harman AN,Bye CR,Nasr N,Sandgren KJ,Kim M,Mercier SK,Botting RA,Lewin SR,Cunningham AL,Cameron PU

12-9039 was used in Flow cytometry/Cell sorting to study the lineage relationships between blood and tissue dendritic cells, providing novel markers of blood and skin human dendritic cells.

Tue Jan 01 00:00:00 EST 2013

Bone marrow mononuclear cells up-regulate toll-like receptor expression and produce inflammatory mediators in response to cigarette smoke extract.

PloS one

Zhou J,Eksioglu EA,Fortenbery NR,Chen X,Wang H,Epling-Burnette PK,Djeu JY,Wei S

12-9039 was used in Flow cytometry/Cell sorting to elucidate the effects of cigarette smoke on human bone marrow haematopoiesis and characterise the inflammatory response.

Thu Dec 01 00:00:00 EST 2011

Porcine small intestinal epithelial cell line (IPEC-J2) of rotavirus infection as a new model for the study of innate immune responses to rotaviruses and probiotics.

Viral immunology

Liu F,Li G,Wen K,Bui T,Cao D,Zhang Y,Yuan L

12-9039 was used in Flow cytometry/Cell sorting to establish a novel in-vitro model of rotavirus infection and probiotic treatment.

Thu Apr 01 00:00:00 EDT 2010

Secretion of the human Toll-like receptor 3 ectodomain is affected by single nucleotide polymorphisms and regulated by Unc93b1.

The Journal of biological chemistry

Qi R,Hoose S,Schreiter J,Sawant KV,Lamb R,Ranjith-Kumar CT,Mills J,San Mateo L,Jordan JL,Kao CC

12-9039 was used in Flow cytometry/Cell sorting to show that a siRNA knockdown of Unc93b1 can reduce TLR3 abundance on the cell surface without affecting total TLR3 accumulation.

Fri Nov 19 00:00:00 EST 2010

Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth.

Proceedings of the National Academy of Sciences of the United States of America

Cho WG,Albuquerque RJ,Kleinman ME,Tarallo V,Greco A,Nozaki M,Green MG,Baffi JZ,Ambati BK,De Falco M,Alexander JS,Brunetti A,De Falco S,Ambati J

12-9039 was used in Flow cytometry/Cell sorting to introduce TLR3 activation as a method of jointly suppressing blood and lymphatic neovascularization.

Tue Apr 28 00:00:00 EDT 2009

Toll-like receptor and innate cytokine responses induced by lactobacilli colonization and human rotavirus infection in gnotobiotic pigs.

Veterinary immunology and immunopathology

Wen K,Azevedo MS,Gonzalez A,Zhang W,Saif LJ,Li G,Yousef A,Yuan L

12-9039 was used in Flow cytometry/Cell sorting to evaluate TLR2-, TLR3- and TLR9-expressing antigen presenting cell responses in the spleens and blood of gnotobiotic pigs.

Sun Feb 15 00:00:00 EST 2009

Biochemical and functional analyses of the human Toll-like receptor 3 ectodomain.

The Journal of biological chemistry

Ranjith-Kumar CT,Miller W,Xiong J,Russell WK,Lamb R,Santos J,Duffy KE,Cleveland L,Park M,Bhardwaj K,Wu Z,Russell DH,Sarisky RT,Mbow ML,Kao CC

12-9039 was used in Flow cytometry/Cell sorting to determine through mutational analysis the biochemical function of human TLR3 ectodomain.

Fri Mar 09 00:00:00 EST 2007

Effects of single nucleotide polymorphisms on Toll-like receptor 3 activity and expression in cultured cells.

The Journal of biological chemistry

Ranjith-Kumar CT,Miller W,Sun J,Xiong J,Santos J,Yarbrough I,Lamb RJ,Mills J,Duffy KE,Hoose S,Cunningham M,Holzenburg A,Mbow ML,Sarisky RT,Kao CC

12-9039 was used in Flow cytometry/Cell sorting to examine the effect of single nucleotide polymorphisms on Toll-like receptor 3 signalling and expression.

Fri Jun 15 00:00:00 EDT 2007

Toll-like receptor 2, 3, and 4 expression and function in human airway smooth muscle.

The Journal of allergy and clinical immunology

Sukkar MB,Xie S,Khorasani NM,Kon OM,Stanbridge R,Issa R,Chung KF

12-9039 was used in Flow cytometry/Cell sorting to determine the effects of cytokines, TLR ligands, and corticosteroids on TLR expression in human airway smooth muscle cells.

Fri Sep 01 00:00:00 EDT 2006

Diesel exhaust enhances virus- and poly(I:C)-induced Toll-like receptor 3 expression and signaling in respiratory epithelial cells.

American journal of physiology. Lung cellular and molecular physiology

Ciencewicki J,Brighton L,Wu WD,Madden M,Jaspers I

12-9039 was used in Flow cytometry/Cell sorting to demonstrate that exposure of respiratory epithelial cells to diesel exhaust can increase the expression and function of TLR3.

Thu Jun 01 00:00:00 EDT 2006

Structural and functional analyses of the human Toll-like receptor 3. Role of glycosylation.

The Journal of biological chemistry

Sun J,Duffy KE,Ranjith-Kumar CT,Xiong J,Lamb RJ,Santos J,Masarapu H,Cunningham M,Holzenburg A,Sarisky RT,Mbow ML,Kao C

12-9039 was used in Flow cytometry/Cell sorting to investigate the relationship between toll-like receptor 3 and glycosylation.

Fri Apr 21 00:00:00 EDT 2006

Expansion of toll-like receptor 9-expressing B cells in active systemic lupus erythematosus: implications for the induction and maintenance of the autoimmune process.

Arthritis and rheumatism

Papadimitraki ED,Choulaki C,Koutala E,Bertsias G,Tsatsanis C,Gergianaki I,Raptopoulou A,Kritikos HD,Mamalaki C,Sidiropoulos P,Boumpas DT

12-9039 was used in Flow cytometry/Cell sorting to examine the expression and function of various TLRs in subpopulations of peripheral blood mononuclear cells of patients with systemic lupus erythematosus.

Wed Nov 01 00:00:00 EST 2006

Toll-like receptor 3 is induced by and mediates antiviral activity against rhinovirus infection of human bronchial epithelial cells.

Journal of virology

Hewson CA,Jardine A,Edwards MR,Laza-Stanca V,Johnston SL

12-9039 was used in Flow cytometry/Cell sorting to demonstrate an important functional requirement for TLR3 in the host response against live virus infection.

Sat Oct 01 00:00:00 EDT 2005

Human endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA.

Human immunology

Jorgenson RL,Young SL,Lesmeister MJ,Lyddon TD,Misfeldt ML

Published figure using CD283 (TLR3) monoclonal antibody (Product # 12-9039-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2005

Human endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA.

Human immunology

Jorgenson RL,Young SL,Lesmeister MJ,Lyddon TD,Misfeldt ML

Published figure using CD283 (TLR3) monoclonal antibody (Product # 12-9039-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2005

17Beta-estradiol suppresses TLR3-induced cytokine and chemokine production in endometrial epithelial cells.

Reproductive biology and endocrinology : RB&E

Lesmeister MJ,Jorgenson RL,Young SL,Misfeldt ML

12-9039 was used in Flow cytometry/Cell sorting to investigate the effects of 17beta-estradiol and progesterone on TLR3 expression and function in endometrial cell lines.

Thu Dec 29 00:00:00 EST 2005

Activation of airway epithelial cells by toll-like receptor agonists.

American journal of respiratory cell and molecular biology

Sha Q,Truong-Tran AQ,Plitt JR,Beck LA,Schleimer RP

12-9039 was used in Flow cytometry/Cell sorting to investigate the presence and function of Toll-like receptors in the BEAS-2B airway epithelial cell line and primary bronchial epithelial cells.

Wed Sep 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: CD283; TLR 3; Toll-like receptor 3

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Gene Aliases: CD283; IIAE2; TLR3

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UniProt ID: (Human) O15455

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Entrez Gene ID: (Human) 7098

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Function(s)

double-stranded RNA binding receptor activity transmembrane signaling receptor activity protein binding identical protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD283 (TLR3) Monoclonal Antibody (TLR3.7), PE, eBioscience™

CD283 (TLR3) Antibody (12-9039-82) in Flow

Product Details

12-9039-82

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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