Product References
miR-206 alleviates LPS-induced inflammatory injury in cardiomyocytes via directly targeting USP33 to inhibit the JAK2/STAT3 signaling pathway.
Molecular and cellular biochemistry
Dong W,Chen J,Wang Y,Weng J,Du X,Fang X,Liu W,Long T,You J,Wang W,Peng X
13-2500 was used in Immunoprecipitation to elucidate the potential mechanism of miR-206 in septic cardiomyopathy (SCM).
Mon Apr 01 00:00:00 UTC 2024
A Msp1-containing complex removes orphaned proteins in the mitochondrial outer membrane of T. brucei.
Life science alliance
Gerber M,Suppanz I,Oeljeklaus S,Niemann M,Käser S,Warscheid B,Schneider A,Dewar CE
13-2500 was used in Immunocytochemistry to demonstrate that not only Msp1 but also the trypanosomal homolog of the AAA-ATPase VCP are implicated in this quality control pathway.
Wed Nov 01 00:00:00 UTC 2023
A Msp1-containing complex removes orphaned proteins in the mitochondrial outer membrane of T. brucei.
Life science alliance
Gerber M,Suppanz I,Oeljeklaus S,Niemann M,Käser S,Warscheid B,Schneider A,Dewar CE
13-2500 was used in Immunocytochemistry to demonstrate that not only Msp1 but also the trypanosomal homolog of the AAA-ATPase VCP are implicated in this quality control pathway.
Wed Nov 01 00:00:00 UTC 2023
LSD1 interacting with HSP90 promotes skin wound healing by inducing metabolic reprogramming of hair follicle stem cells through the c-MYC/LDHA axis.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Li S,Yu J,Zhang J,Li X,Yu J
13-2500 was used in Immunoprecipitation to conclude that LSD1 interacting with HSP90 accelerates skin wound healing by inducing HFSC glycolytic metabolism, proliferation, and differentiation via c-MYC/LDHA axis.
Sat Jul 01 00:00:00 UTC 2023
LSD1 interacting with HSP90 promotes skin wound healing by inducing metabolic reprogramming of hair follicle stem cells through the c-MYC/LDHA axis.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Li S,Yu J,Zhang J,Li X,Yu J
13-2500 was used in Immunoprecipitation to conclude that LSD1 interacting with HSP90 accelerates skin wound healing by inducing HFSC glycolytic metabolism, proliferation, and differentiation via c-MYC/LDHA axis.
Sat Jul 01 00:00:00 UTC 2023
Yeast transcription factor Msn2 binds to G4 DNA.
Nucleic acids research
Duy DL,Kim N
13-2500 was used in ChIP assay to suggest that G4 DNA could be an alternative binding site of Msn2 in addition to stress response element, and that G4 DNA formation could be an important element of transcriptional regulation in yeast.
Fri Oct 13 00:00:00 UTC 2023
Yeast transcription factor Msn2 binds to G4 DNA.
Nucleic acids research
Duy DL,Kim N
13-2500 was used in ChIP assay to suggest that G4 DNA could be an alternative binding site of Msn2 in addition to stress response element, and that G4 DNA formation could be an important element of transcriptional regulation in yeast.
Fri Oct 13 00:00:00 UTC 2023
Taspase1 Facilitates Topoisomerase IIβ-Mediated DNA Double-Strand Breaks Driving Estrogen-Induced Transcription.
Cells
Oelschläger L,Stahl P,Kaschani F,Stauber RH,Knauer SK,Hensel A
13-2500 was used in ELISA, Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to characterize Taspase1 as a multifunctional co-activator of estrogen-stimulated transcription.
Wed Jan 18 00:00:00 UTC 2023
The endoplasmic reticulum kinase PERK interacts with the oxidoreductase ERO1 to metabolically adapt mitochondria.
Cell reports
Bassot A,Chen J,Takahashi-Yamashiro K,Yap MC,Gibhardt CS,Le GNT,Hario S,Nasu Y,Moore J,Gutiérrez T,Mina L,Mast H,Moses A,Bhat R,Ballanyi K,Lemieux H,Sitia R,Zito E,Bogeski I,Campbell RE,Simmen T
13-2500 was used in ELISA, Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Protein Assay to conclude that the PERK-ERO1α complex is a key molecular machinery that allows quick metabolic adaptation to ER stress.
Tue Jan 31 00:00:00 UTC 2023
METTL3 regulates breast cancer-associated alternative splicing switches.
Oncogene
Achour C,Bhattarai DP,Groza P,Román ÁC,Aguilo F
13-2500 was used in Western Blotting to identify a specific alternative splicing signature associated with breast tumorigenesis in vitro.
Wed Mar 01 00:00:00 UTC 2023
RAS-independent ERK activation by constitutively active KSR3 in non-chordate metazoa.
Nature communications
Chessel A,De Crozé N,Molina MD,Taberner L,Dru P,Martin L,Lepage T
13-2500 was used in Immunoprecipitation to reveal key functions for this family of factors as activators of RAF in RAS-independent ERK signalling in invertebrates.
Wed Jul 05 00:00:00 UTC 2023
ARMS-NF-κB signaling regulates intracellular ROS to induce autophagy-associated cell death upon oxidative stress.
iScience
Liao YH,Wu JT,Hsieh IC,Lee HH,Huang PH
13-2500 was used in Flow Cytometry, ELISA, Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to establish ARMS-knockdown in melanoma cells (siARMS) and in Drosophila eyes (GMR>dARMS RNAi ) and challenged them with H2O2.
Fri Feb 17 00:00:00 UTC 2023
A comprehensive SARS-CoV-2-human protein-protein interactome reveals COVID-19 pathobiology and potential host therapeutic targets.
Nature biotechnology
Zhou Y,Liu Y,Gupta S,Paramo MI,Hou Y,Mao C,Luo Y,Judd J,Wierbowski S,Bertolotti M,Nerkar M,Jehi L,Drayman N,Nicolaescu V,Gula H,Tay S,Randall G,Wang P,Lis JT,Feschotte C,Erzurum SC,Cheng F,Yu H
13-2500 was used in ELISA, Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to demonstrate the value of network systems biology to understand human-virus interactions and provides hits for further research on COVID-19 therapeutics.
Sun Jan 01 00:00:00 UTC 2023
A comprehensive SARS-CoV-2-human protein-protein interactome reveals COVID-19 pathobiology and potential host therapeutic targets.
Nature biotechnology
Zhou Y,Liu Y,Gupta S,Paramo MI,Hou Y,Mao C,Luo Y,Judd J,Wierbowski S,Bertolotti M,Nerkar M,Jehi L,Drayman N,Nicolaescu V,Gula H,Tay S,Randall G,Wang P,Lis JT,Feschotte C,Erzurum SC,Cheng F,Yu H
13-2500 was used in ELISA, Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to demonstrate the value of network systems biology to understand human-virus interactions and provides hits for further research on COVID-19 therapeutics.
Sun Jan 01 00:00:00 UTC 2023
An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold.
PLoS biology
Wong W,Estep JA,Treptow AM,Rajabli N,Jahncke JN,Ubina T,Wright KM,Riccomagno MM
13-2500 was used in Immunocytochemistry to establish a new and essential role for Cas adaptor proteins during the formation of cortical circuits and reveal a signaling axis controlling cortical scaffold formation.
Tue Aug 01 00:00:00 UTC 2023
DNMT3A-coordinated splicing governs the stem state switch towards differentiation in embryonic and haematopoietic stem cells.
Nature cell biology
Ramabadran R,Wang JH,Reyes JM,Guzman AG,Gupta S,Rosas C,Brunetti L,Gundry MC,Tovy A,Long H,Gu T,Cullen SM,Tyagi S,Rux D,Kim JJ,Kornblau SM,Kyba M,Stossi F,Rau RE,Takahashi K,Westbrook TF,Goodell MA
13-2500 was used in Western Blot to reveal a modality through which DNA methyltransferase 3α and the spliceosome govern exit from the stem state towards differentiation.
Sat Apr 01 00:00:00 UTC 2023
USP25 inhibition ameliorates Alzheimer's pathology through the regulation of APP processing and Aβ generation.
The Journal of clinical investigation
Zheng Q,Song B,Li G,Cai F,Wu M,Zhao Y,Jiang L,Guo T,Shen M,Hou H,Zhou Y,Zhao Y,Di A,Zhang L,Zeng F,Zhang XF,Luo H,Zhang X,Zhang H,Zeng Z,Huang TY,Dong C,Qing H,Zhang Y,Zhang Q,Wang X,Wu Y,Xu H,Song W,Wang X
13-2500 was used in Western Blot to suggest that USP25 serves as a potential pharmacological target for AD drug development.
Tue Mar 01 00:00:00 UTC 2022
A network of Gαi signaling partners is revealed by proximity labeling proteomics analysis and includes PDZ-RhoGEF.
Science signaling
Chandan NR,Abraham S,SenGupta S,Parent CA,Smrcka AV
13-2500 was used in Functional Assay to suggest that the identification and characterization of new targets directly or indirectly regulated by Gαi will aid in the investigation of the functional roles of Gαi-coupled GPCRs in multiple biological processes.
Tue Jan 18 00:00:00 UTC 2022
A Multi-Specific DARPin Potently Neutralizes Shiga Toxin 2 via Simultaneous Modulation of Both Toxin Subunits.
Bioengineering (Basel, Switzerland)
Zeng Y,Jiang M,Robinson S,Peng Z,Chonira V,Simeon R,Tzipori S,Zhang J,Chen Z
13-2500 was used in ELISA to report the engineering of a panel of designed ankyrin repeat proteins (DARPin) with potent neutralization activity against Stx2a, the major subtype associated with HUS.
Tue Sep 27 00:00:00 UTC 2022
High-resolution crystal structure and chemical screening reveal pantothenate kinase as a new target for antifungal development.
Structure (London, England : 1993)
Gihaz S,Gareiss P,Choi JY,Renard I,Pal AC,Surovsteva Y,Chiu JE,Thekkiniath J,Plummer M,Hungerford W,Montgomery ML,Hosford A,Adams EM,Lightfoot JD,Fox D,Ojo KK,Staker BL,Fuller K,Ben Mamoun C
13-2500 was used in Western Blot to report the crystal structure of a fungal PanK alone as well as with high-affinity inhibitors from a single chemotype identified through a high-throughput chemical screen.
Thu Nov 03 00:00:00 UTC 2022
Multi-omics profiling of collagen-induced arthritis mouse model reveals early metabolic dysregulation via SIRT1 axis.
Scientific reports
Li L,Freitag J,Asbrand C,Munteanu B,Wang BT,Zezina E,Didier M,Thill G,Rocher C,Herrmann M,Biesemann N
13-2500 was used in Western Blotting to study the longitudinal metabolic changes during the development of murine arthritis by integrating metabolomics and transcriptomics data.
Tue Jul 12 00:00:00 UTC 2022
Impaired oxygen-sensitive regulation of mitochondrial biogenesis within the von Hippel-Lindau syndrome.
Nature metabolism
Li S,Li W,Yuan J,Bullova P,Wu J,Zhang X,Liu Y,Plescher M,Rodriguez J,Bedoya-Reina OC,Jannig PR,Valente-Silva P,Yu M,Henriksson MA,Zubarev RA,Smed-Sörensen A,Suzuki CK,Ruas JL,Holmberg J,Larsson C,Christofer Juhlin C,von Kriegsheim A,Cao Y,Schlisio S
13-2500 was used in Western Blotting to offer pharmacological avenues to sensitize therapy-resistant VHL tumours by focusing on the mitochondria.
Wed Jun 01 00:00:00 UTC 2022
Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis.
Cell discovery
Chen Q,Ma K,Liu X,Chen SH,Li P,Yu Y,Leung AKL,Yu X
13-2500 was used in Western Blot to reveal a novel biological role of tPARP1 during cytosolic DNA-induced apoptosis.
Tue Jan 18 00:00:00 UTC 2022
Androgen receptor-mediated transcriptional repression targets cell plasticity in prostate cancer.
Molecular oncology
Erdmann É,Ould Madi Berthélémy P,Cottard F,Angel CZ,Schreyer E,Ye T,Morlet B,Negroni L,Kieffer B,Céraline J
13-2500 was used in Western Blotting to analyze and compare the transcriptomes of the wild-type and variant forms of the androgen receptor (AR) in prostate cancer cells, revealing that the wild-type AR had a repressive function on genes related to cell adhesion and epithelial-to-mesenchymal transition, which was pathologically lost in AR variants.
Fri Jul 01 00:00:00 UTC 2022
Astrocytes show increased levels of Ero1α in multiple sclerosis and its experimental autoimmune encephalomyelitis animal model.
The European journal of neuroscience
Samtleben S,Mina L,Yap MC,Branton WG,Yousuf MS,Tenorio G,Ballanyi K,Giuliani F,Kerr BJ,Power C,Simmen T
13-2500 was used in ELISA, Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that Ero1α induction makes astrocytes vulnerable to oxidative stress in the MS and EAE pathologies.
Sat Oct 01 00:00:00 UTC 2022
Secretory autophagy machinery and vesicular trafficking are involved in HMGB1 secretion.
Autophagy
Kim YH,Kwak MS,Lee B,Shin JM,Aum S,Park IH,Lee MG,Shin JS
13-2500 was used in Western Blotting to show secretory autophagy and multivesicular body formation mediate HMGB1 secretion.
Wed Sep 01 00:00:00 UTC 2021
The proppin Bcas3 and its interactor KinkyA localize to the early phagophore and regulate autophagy.
Autophagy
Yamada Y,Schaap P
13-2500 was used in Immunoprecipitation to isolate a mutant in kinkyA (knkA), a gene without known function, which formed fruiting bodies with a kinked stalk and lacking viable spores.
Mon Mar 01 00:00:00 UTC 2021
Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration.
Current biology : CB
Calero-Cuenca FJ,Osorio DS,Carvalho-Marques S,Sridhara SC,Oliveira LM,Jiao Y,Diaz J,Janota CS,Cadot B,Gomes ER
13-2500 was used in Immunocytochemistry to study Ctdnep1 and Eps8L2 interactions and demonstrate how these interactions are important for nuclear positioning and cell migration.
Mon Apr 12 00:00:00 UTC 2021
TMEM41B and VMP1 are scramblases and regulate the distribution of cholesterol and phosphatidylserine.
The Journal of cell biology
Li YE,Wang Y,Du X,Zhang T,Mak HY,Hancock SE,McEwen H,Pandzic E,Whan RM,Aw YC,Lukmantara IE,Yuan Y,Dong X,Don A,Turner N,Qi S,Yang H
13-2500 was used in Western Blot to show that TMEM41B and VMP1 are phospholipid scramblases whose deficiency impairs the normal cellular distribution of cholesterol and phosphatidylserine.
Mon Jun 07 00:00:00 UTC 2021
Paramyxovirus-Like Particles as Protein Delivery Vehicles.
Journal of virology
Panthi S,Schmitt PT,Lorenz FJ,Stanfield BA,Schmitt AP
13-2500 was used in Western Blotting to develop a new strategy for protein delivery based on manipulation of paramyxovirus genome packaging interactions.
Mon Sep 27 00:00:00 UTC 2021
Kog1/Raptor mediates metabolic rewiring during nutrient limitation by controlling SNF1/AMPK activity.
Science advances
Rashida Z,Srinivasan R,Cyanam M,Laxman S
13-2500 was used in Western Blotting to identify a modified form of the yeast TORC1 component Kog1/Raptor, which exhibits delayed growth exclusively during glucose and amino acid limitations.
Thu Apr 01 00:00:00 UTC 2021
Highly Conserved C-Terminal Region of Indian Hedgehog N-Fragment Contributes to Its Auto-Processing and Multimer Formation.
Biomolecules
Wang X,Liu H,Liu Y,Han G,Wang Y,Chen H,He L,Ma G
13-2500 was used in Western Blot to focus on Indian Hedgehog (IHH), to explore the functions of the C-terminal region of the amino-terminal fragment of IHH (IHH-N) via protein truncation, cell-based assays, and 3D structure prediction.
Tue May 25 00:00:00 UTC 2021
Interactome Mapping of eIF3A in a Colon Cancer and an Immortalized Embryonic Cell Line Using Proximity-Dependent Biotin Identification.
Cancers
Vo DK,Engler A,Stoimenovski D,Hartig R,Kaehne T,Kalinski T,Naumann M,Haybaeck J,Nass N
13-2500 was used in Western Blot to analyse the interactions of eukaryotic initiation factors (eIFs) using eIF3A as a prototype.
Sun Mar 14 00:00:00 UTC 2021
DHODH inhibition modulates glucose metabolism and circulating GDF15, and improves metabolic balance.
iScience
Zhang J,Terán G,Popa M,Madapura H,Ladds MJGW,Lianoudaki D,Grünler J,Arsenian-Henriksson M,McCormack E,Rottenberg ME,Catrina SB,Laín S,Darekar S
13-2500 was used in ELISA, Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that pharmacological inhibition of DHODH reduces mitochondrial respiration, promotes glycolysis, and enhances GLUT4 translocation to the cytoplasmic membrane and that by activating tumor suppressor p53, increases the expression of GDF15, a cytokine that reduces appetite and prolongs lifespan.
Fri May 21 00:00:00 UTC 2021
ADP-ribosylation of histone variant H2AX promotes base excision repair.
The EMBO journal
Chen Q,Bian C,Wang X,Liu X,Ahmad Kassab M,Yu Y,Yu X
13-2500 was used in ELISA, Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to identify the glutamate residue 141 (E141) of variant histone H2AX is ADP-ribosylated following oxidative DNA damage.
Fri Jan 15 00:00:00 UTC 2021
P2X7 Receptors and TMEM16 Channels Are Functionally Coupled with Implications for Macropore Formation and Current Facilitation.
International journal of molecular sciences
Dunning K,Martz A,Peralta FA,Cevoli F,Boué-Grabot E,Compan V,Gautherat F,Wolf P,Chataigneau T,Grutter T
13-2500 was used in Western Blot to provide evidence that current facilitation and macropore formation involve functional complexes comprised of P2X7 and TMEM16, a family of Ca2+-activated ion channel/scramblases.
Fri Jun 18 00:00:00 UTC 2021
The Arabidopsis leucine-rich repeat receptor-like kinase MIK2 is a crucial component of early immune responses to a fungal-derived elicitor.
The New phytologist
Coleman AD,Maroschek J,Raasch L,Takken FLW,Ranf S,Hückelhoven R
13-2500 was used in Western Blot to increase mechanistic understanding of PTI to Fusarium and place MIK2 at a central position in Arabidopsis elicitor responses.
Mon Mar 01 00:00:00 UTC 2021
Topological stress is responsible for the detrimental outcomes of head-on replication-transcription conflicts.
Cell reports
Lang KS,Merrikh H
13-2500 was used in Western Blot to suggest that after positive supercoil resolution, gyrase introduces excessive negative supercoils at head-on conflict regions, driving pervasive R-loop formation.
Tue Mar 02 00:00:00 UTC 2021
TRPV2-spike protein interaction mediates the entry of SARS-CoV-2 into macrophages in febrile conditions.
Theranostics
Xu J,Yang Y,Hou Z,Jia H,Wang Y
13-2500 was used in Western Blot to identify TRPV2 as a receptor of SARS-CoV-2 in conditions of febrile temperature, providing insight into critical interactions of SARS-CoV-2 with macrophages, as well as a useful resource and potential drug target for coronavirus disease.
Wed Jun 30 00:00:00 UTC 2021
Persistently Elevated mTOR Complex 1-S6 Kinase 1 Disrupts DARPP-32-Dependent D1 Dopamine Receptor Signaling and Behaviors.
Biological psychiatry
Lin R,Learman LN,Na CH,Renuse S,Chen KT,Chen PY,Lee GH,Xiao B,Resnick SM,Troncoso JC,Szumlinski KK,Linden DJ,Park JM,Savonenko A,Pandey A,Worley PF
13-2500 was used in Western Blotting to demonstrate that the mTORC1-S6K1 intersection with D1R signaling provides a molecular framework to understand the effects of pathological mTORC1 activation on behavioral symptoms in neuropsychiatric disease.
Tue Jun 01 00:00:00 UTC 2021
Fructose-1,6-Bisphosphatase 2 Inhibits Sarcoma Progression by Restraining Mitochondrial Biogenesis.
Cell metabolism
Huangyang P,Li F,Lee P,Nissim I,Weljie AM,Mancuso A,Li B,Keith B,Yoon SS,Simon MC
13-2500 was used in Chromatin immunoprecipitation to show that expression of the gluconeogenic isozyme fructose-1,6-bisphosphatase 2 (FBP2) is silenced in a broad spectrum of sarcoma subtypes, revealing an apparent common metabolic feature shared by diverse STSs.
Tue Jan 07 00:00:00 UTC 2020
Directed RNase H Cleavage of Nascent Transcripts Causes Transcription Termination.
Molecular cell
Lai F,Damle SS,Ling KK,Rigo F
13-2500 was used in Western Blotting to show intron- and exon-targeted antisense oligonucleotides cause RNA polymerase II transcription termination in cultured cells and mice.
Thu Mar 05 00:00:00 UTC 2020
Argonaute binding within 3'-untranslated regions poorly predicts gene repression.
Nucleic acids research
Chu Y,Kilikevicius A,Liu J,Johnson KC,Yokota S,Corey DR
13-2500 was used in Western Blotting to identify potential microRNA (miRNA) binding sites.
Mon Jul 27 00:00:00 UTC 2020
Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments.
Current biology : CB
Gutierrez A,Kim JO,Umbreit NT,Asbury CL,Davis TN,Miller MP,Biggins S
13-2500 was used in Western Blotting to show Cdk1, the major mitotic kinase that drives the cell cycle, enhances kinetochore-microtubule attachment strength by increasing the residence time of Dam1c via Ask1.
Mon Nov 16 00:00:00 UTC 2020
TMEM132A, a Novel Wnt Signaling Pathway Regulator Through Wntless (WLS) Interaction.
Frontiers in cell and developmental biology
Li B,Niswander LA
13-2500 was used in Western Blotting to show that TMEM132A stabilizes Wnt ligand, enhances WLS-Wnt ligand interaction, and activates the Wnt signaling pathway.
Fri May 14 00:00:00 UTC 2021
Image-Based Analysis of Protein Stability.
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Hickman KA,Hariharan S,De Melo J,Ylanko J,Lustig LC,Penn LZ,Andrews DW
13-2500 was used in Western Blotting to develop a protein stability probe and high-content screening pipeline for novel regulators of short half-life proteins using automated image analysis.
Wed Apr 01 00:00:00 UTC 2020
TRIM47 Promotes the Development of Glioma by Ubiquitination and Degradation of FOXO1.
OncoTargets and therapy
Wei H,Ding C,Zhuang H,Hu W
13-2500 was used in Western Blotting to confirm the functional role of the TRIM47-FOXO1 axis in the progression of gliomas and provided a potential target for glioma treatment.
Tue Apr 19 00:00:00 UTC 2022
ERK Regulates NeuroD1-mediated Neurite Outgrowth via Proteasomal Degradation.
Experimental neurobiology
Lee TY,Cho IS,Bashyal N,Naya FJ,Tsai MJ,Yoon JS,Choi JM,Park CH,Kim SS,Suh-Kim H
13-2500 was used in Western Blotting to provide evidence to indicate that a longer protein half-life enhanced the overall activity of NeuroD1 in stimulating downstream genes and neuronal differentiation.
Tue Jun 30 00:00:00 UTC 2020
Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula.
PloS one
Azarakhsh M,Rumyantsev AM,Lebedeva MA,Lutova LA
13-2500 was used in Western Blotting to suggest that MtKNOX3 TF may directly activate the MtLOG1, MtLOG2, and MtIPT3 genes during nodulation thereby increasing cytokinin biosynthesis in developing nodules.
Mon Jul 27 00:00:00 UTC 2020
The yeast cell wall protein Pry3 inhibits mating through highly conserved residues within the CAP domain.
Biology open
Cottier S,Darwiche R,Meyenhofer F,Debelyy MO,Schneiter R
13-2500 was used in Western Blotting to study Pry3's function in yeast cells.
Thu Jul 02 00:00:00 UTC 2020
CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.
Cell reports
Gurkaslar HK,Culfa E,Arslanhan MD,Lince-Faria M,Firat-Karalar EN
13-2500 was used in Western Blotting to demonstrate that CCDC57 provides a vital interface between centrosome and microtubule-mediated cellular processes.
Tue May 12 00:00:00 UTC 2020
Genomic deregulation of PRMT5 supports growth and stress tolerance in chronic lymphocytic leukemia.
Scientific reports
Schnormeier AK,Pommerenke C,Kaufmann M,Drexler HG,Koeppel M
13-2500 was used in Western Blotting to develop a comprehensive approach combining a variety of molecular and clinical data to pinpoint translocation events disrupting long-range chromatin interactions and causing cancer-relevant transcriptional deregulation.
Wed Jun 17 00:00:00 UTC 2020
A tudor domain protein, SIMR-1, promotes siRNA production at piRNA-targeted mRNAs in C. elegans.
eLife
Manage KI,Rogers AK,Wallis DC,Uebel CJ,Anderson DC,Nguyen DAH,Arca K,Brown KC,Cordeiro Rodrigues RJ,de Albuquerque BF,Ketting RF,Montgomery TA,Phillips CM
13-2500 was used in Western Blotting to suggest a role for multiple perinuclear condensates in organizing the piRNA pathway and promoting mRNA regulation by the mutator complex.
Mon Apr 27 00:00:00 UTC 2020
The F-box protein FBXL16 up-regulates the stability of C-MYC oncoprotein by antagonizing the activity of the F-box protein FBW7.
The Journal of biological chemistry
Morel M,Shah KN,Long W
13-2500 was used in Western Blotting to reveal that FBXL16 is an F-box protein that antagonizes the activity of another F-box protein, FBW7, and thereby increases C-MYC stability, resulting in increased cancer cell growth and invasiveness.
Fri Jun 05 00:00:00 UTC 2020
Correction: Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula.
PloS one
13-2500 was used in Western Blotting to find that MtKNOX3 homeodomain directly binds to the regulatory sequences of the MtLOG1, MtLOG2, and MtIPT3 genes involved in nodulation in Medicago truncatula.
Wed Aug 05 00:00:00 UTC 2020
Yeast surface display of full-length human microtubule-associated protein tau.
Biotechnology progress
Wang S,Cho YK
13-2500 was used in Immunocytochemistry to show that yeast-displayed tau enables robust measurement of protein interactions and is of particular interest for characterizing conformational change.
Wed Jan 01 00:00:00 UTC 2020
Yeast surface display of full-length human microtubule-associated protein tau.
Biotechnology progress
Wang S,Cho YK
13-2500 was used in Immunocytochemistry to show that yeast-displayed tau enables robust measurement of protein interactions and is of particular interest for characterizing conformational change.
Wed Jan 01 00:00:00 UTC 2020
The single CCA-adding enzyme of T. brucei has distinct functions in the cytosol and in mitochondria.
The Journal of biological chemistry
Shikha S,Schneider A
13-2500 was used in Western Blot, Immunocytochemistry to indicate that the yeast enzyme complements the essential function of TbCAE by adding CCA to the primary tRNA transcripts.
Fri May 01 00:00:00 UTC 2020
The single CCA-adding enzyme of T. brucei has distinct functions in the cytosol and in mitochondria.
The Journal of biological chemistry
Shikha S,Schneider A
13-2500 was used in Western Blot, Immunocytochemistry to indicate that the yeast enzyme complements the essential function of TbCAE by adding CCA to the primary tRNA transcripts.
Fri May 01 00:00:00 UTC 2020
A cell-free antibody engineering platform rapidly generates SARS-CoV-2 neutralizing antibodies.
bioRxiv : the preprint server for biology
Chen X,Gentili M,Hacohen N,Regev A
13-2500 was used in GST Pull Down to apply CeVICA to engineer antibodies against the Receptor Binding Domain (RBD) of the SARS-CoV-2 spike proteins and identify >800 predicted binder families.
Fri Oct 30 00:00:00 UTC 2020
The LIM protein Ajuba recruits DBC1 and CBP/p300 to acetylate ERα and enhances ERα target gene expression in breast cancer cells.
Nucleic acids research
Xu B,Li Q,Chen N,Zhu C,Meng Q,Ayyanathan K,Qian W,Jia H,Wang J,Ni P,Hou Z
13-2500 was used in Western Blotting to study the mechanisms by which Ajuba potentiates oestrogen receptor α target gene expression, promotes breast cancer cell growth, and contributes to tamoxifen resistance in these cells.
Mon Mar 18 00:00:00 UTC 2019
Selection and characterization of ultrahigh potency designed ankyrin repeat protein inhibitors of C. difficile toxin B.
PLoS biology
Simeon R,Jiang M,Chamoun-Emanuelli AM,Yu H,Zhang Y,Meng R,Peng Z,Jakana J,Zhang J,Feng H,Chen Z
13-2500 was used in an ELISA assay to report the engineering of a series of monomeric and dimeric designed ankyrin repeat proteins for the neutralization of TcdB.
Sat Jun 01 00:00:00 UTC 2019
Involvement of PDZ-SAP97 interactions in regulating AQP2 translocation in response to vasopressin in LLC-PK1 cells.
American journal of physiology. Renal physiology
Nooh MM,Kale A,Bahouth SW
13-2500 was used in Western Blotting to investigate if the targeting of PKA to the microdomain of aquaporin-2 (AQP2) via synapse-associated protein-97-AQP2 interactions in association with cross-talk between two barcodes in AQP2, namely, the type-1 PDZ-binding motif and phospho-Ser256, plays an important role in the translocation of AQP2 in the kidney.
Thu Aug 01 00:00:00 UTC 2019
Designed Ankyrin Repeat Protein (DARPin) Neutralizers of TcdB from Clostridium difficile Ribotype 027.
mSphere
Peng Z,Simeon R,Mitchell SB,Zhang J,Feng H,Chen Z
13-2500 was used in an ELISA assay to describe the engineering of a panel of designed ankyrin repeat proteins (DARPins) that potently neutralize TcdB from the BI/NAP1/027 strains of Clostridium difficile.
Wed Oct 02 00:00:00 UTC 2019
Engineering of anti-human interleukin-4 receptor alpha antibodies with potent antagonistic activity.
Scientific reports
Kim JE,Jung K,Kim JA,Kim SH,Park HS,Kim YS
13-2500 was used in Flow cytometry/Cell sorting to demonstrate that both affinity and epitope are critical factors for the efficacy of anti-IL-4Rα antagonistic Abs.
Thu May 23 00:00:00 UTC 2019
Molecular architecture of the Bardet-Biedl syndrome protein 2-7-9 subcomplex.
The Journal of biological chemistry
Ludlam WG,Aoba T,Cuéllar J,Bueno-Carrasco MT,Makaju A,Moody JD,Franklin S,Valpuesta JM,Willardson BM
13-2500 was used in Immunoprecipitation to analyze the structure of a BBSome 2-7-9 subcomplex consisting of three homologous BBS (Bardet-Biedl syndrome) proteins, BBS2, BBS7, and BBS9, using an integrative structural modelling approach.
Fri Nov 01 00:00:00 UTC 2019
Novel Functions of IFI44L as a Feedback Regulator of Host Antiviral Responses.
Journal of virology
DeDiego ML,Martinez-Sobrido L,Topham DJ
13-2500 was used in Western Blot to describe a completely novel function for IFI44L in negatively modulating innate immune responses induced after virus infections.
Fri Nov 01 00:00:00 UTC 2019
Domain-interface dynamics of CFTR revealed by stabilizing nanobodies.
Nature communications
Sigoillot M,Overtus M,Grodecka M,Scholl D,Garcia-Pino A,Laeremans T,He L,Pardon E,Hildebrandt E,Urbatsch I,Steyaert J,Riordan JR,Govaerts C
13-2500 was used in Flow cytometry/Cell sorting to uncover a conformation of CFTR, involving detachment of NBD1 from the transmembrane domain, which contrast with the compact assembly observed in cryo-EM structures.
Fri Jun 14 00:00:00 UTC 2019
Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization.
Nature communications
Xie Y,Sun J,Han X,Turšić-Wunder A,Toh JDW,Hong W,Gao YG,Miao Y
13-2500 was used in Western Blotting to identify a previously functionally unknown polarisome member, actin-interacting-protein 5, which promotes actin assembly synergistically with formin Bni1.
Thu Nov 07 00:00:00 UTC 2019
Cytoskeletal Associated Filamin A and RhoA Affect Neural Progenitor Specification During Mitosis.
Cerebral cortex (New York, N.Y. : 1991)
Lian G,Wong T,Lu J,Hu J,Zhang J,Sheen V
13-2500 was used in Western Blot to report that functional loss of FlnA not only affects the rate of proliferation by altering cell cycle length but also causes a defect in early differentiation through changes in cell fate specification.
Fri Mar 01 00:00:00 UTC 2019
Interferon-Induced Protein 44 Interacts with Cellular FK506-Binding Protein 5, Negatively Regulates Host Antiviral Responses, and Supports Virus Replication.
mBio
DeDiego ML,Nogales A,Martinez-Sobrido L,Topham DJ
13-2500 was used in Western Blotting to investigate if interferon (IFN)-induced protein 44 (IFI44) positively affects virus production and if it negatively modulates innate immune responses induced after viral infections.
Tue Aug 27 00:00:00 UTC 2019
The Circadian tau Mutation in Casein Kinase 1 Is Part of a Larger Domain That Can Be Mutated to Shorten Circadian Period.
International journal of molecular sciences
Venkatesan A,Fan JY,Bouyain S,Price JL
13-2500 was used in Western Blotting to study the effects of the mutation of residues close to or part of the phosphate recognition site or nuclear localisation signal in casein kinase I.
Thu Feb 14 00:00:00 UTC 2019
Peroxiredoxin-mediated disulfide bond formation is required for nucleocytoplasmic translocation and secretion of HMGB1 in response to inflammatory stimuli.
Redox biology
Kwak MS,Kim HS,Lkhamsuren K,Kim YH,Han MG,Shin JM,Park IH,Rhee WJ,Lee SK,Rhee SG,Shin JS
13-2500 was used in Western Blotting to show that H2O2-induced oxidation of HMGB1, which results in the formation of an intramolecular disulfide bond between Cys23 and Cys45, is necessary and sufficient for its nucleocytoplasmic translocation and secretion.
Sat Jun 01 00:00:00 UTC 2019
Molecular basis of titin exon exclusion by RBM20 and the novel titin splice regulator PTB4.
Nucleic acids research
Dauksaite V,Gotthardt M
13-2500 was used in Western Blotting to study the molecular basis and regulation of RNA-binding motif protein 20-dependent titin exon exclusion.
Fri Jun 01 00:00:00 UTC 2018
TREM2 Is a Receptor for β-Amyloid that Mediates Microglial Function.
Neuron
Zhao Y,Wu X,Li X,Jiang LL,Gui X,Liu Y,Sun Y,Zhu B,Piña-Crespo JC,Zhang M,Zhang N,Chen X,Bu G,An Z,Huang TY,Xu H
13-2500 was used in Western Blotting to determine the neurobiological functions of TREM2 and its physiological ligands.
Wed Mar 07 00:00:00 UTC 2018
High affinity single-chain variable fragments are specific and versatile targeting motifs for extracellular vesicles.
Nanoscale
Longatti A,Schindler C,Collinson A,Jenkinson L,Matthews C,Fitzpatrick L,Blundy M,Minter R,Vaughan T,Shaw M,Tigue N
13-2500 was used in Western Blotting to study the potential of single chain variable fragments as targeting domain to deliver exosomes to cells expressing a cognate antigen.
Tue Aug 07 00:00:00 UTC 2018
Bmp2 and Notch cooperate to pattern the embryonic endocardium.
Development (Cambridge, England)
Papoutsi T,Luna-Zurita L,Prados B,Zaffran S,de la Pompa JL
13-2500 was used in Western Blotting to study how ectopic Bmp2 expression in the mouse chamber myocardium changes the transcriptional signature of adjacent chamber endocardial cells into valve tissue, and enables them to undergo epithelial-mesenchyme transition.
Mon Jul 02 00:00:00 UTC 2018
Inflachromene inhibits autophagy through modulation of Beclin 1 activity.
Journal of cell science
Kim YH,Kwak MS,Shin JM,Hayuningtyas RA,Choi JE,Shin JS
13-2500 was used in Western blotting following immunoprecipitation to study the effect of inflachromene on autophagy.
Tue Feb 20 00:00:00 UTC 2018
Inflachromene inhibits autophagy through modulation of Beclin 1 activity.
Journal of cell science
Kim YH,Kwak MS,Shin JM,Hayuningtyas RA,Choi JE,Shin JS
13-2500 was used in Western blotting following immunoprecipitation to study the effect of inflachromene on autophagy.
Tue Feb 20 00:00:00 UTC 2018
C8orf46 homolog encodes a novel protein Vexin that is required for neurogenesis in Xenopus laevis.
Developmental biology
Moore KB,Logan MA,Aldiri I,Roberts JM,Steele M,Vetter ML
13-2500 was used in Immunohistochemistry-immunofluorescence to characterise vexin, a neural basic helix-loop helix target gene in Xenopus laevis that is homologous to human c8orf46 and is conserved across vertebrate species.
Tue May 01 00:00:00 UTC 2018
A biosensor-based framework to measure latent proteostasis capacity.
Nature communications
Wood RJ,Ormsby AR,Radwan M,Cox D,Sharma A,Vöpel T,Ebbinghaus S,Oliveberg M,Reid GE,Dickson A,Hatters DM
13-2500 was used in Western Blotting to reveal that HSP70 chaperone cycle is rate limited by HSP70 holdase activity under normal conditions, but can be overcome by increased levels of BAG1 nucleotide exchange factor to HSPA1A or activation of the heat shock gene cluster.
Thu Jan 18 00:00:00 UTC 2018
The binding of Chp2's chromodomain to methylated H3K9 is essential for Chp2's role in heterochromatin assembly in fission yeast.
PloS one
Maksimov V,Oya E,Tanaka M,Kawaguchi T,Hachisuka A,Ekwall K,Bjerling P,Nakayama JI
13-2500 was used in Western Blotting to demonstrate that Chp2's lysine 9-methylated histone H3 (H3K9me) binding is critical for SHREC function and that the two SHREC complex modules are interdependent.
Thu Jan 17 00:00:00 UTC 2019
SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity.
Nature communications
Orosa B,Yates G,Verma V,Srivastava AK,Srivastava M,Campanaro A,De Vega D,Fernandes A,Zhang C,Lee J,Bennett MJ,Sadanandom A
13-2500 was used in Immunoprecipitation to study the role of flagellin in promoting the dissociation of BIK1 and intracellular immune signalling in Arabidopsis, and the mechanism involved.
Wed Dec 05 00:00:00 UTC 2018
Structural basis for arginine glycosylation of host substrates by bacterial effector proteins.
Nature communications
Park JB,Kim YH,Yoo Y,Kim J,Jun SH,Cho JW,El Qaidi S,Walpole S,Monaco S,García-García AA,Wu M,Hays MP,Hurtado-Guerrero R,Angulo J,Hardwidge PR,Shin JS,Cho HS
13-2500 was used in Western Blotting to characterise the glycosyltransferase domains of bacterial effector proteins, SseK and NleB.
Tue Oct 16 00:00:00 UTC 2018
Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei.
PLoS pathogens
Käser S,Willemin M,Schnarwiler F,Schimanski B,Poveda-Huertes D,Oeljeklaus S,Haenni B,Zuber B,Warscheid B,Meisinger C,Schneider A
13-2500 was used in Immunofluorescence to study how in trypanosomes the highly conserved β-barrel protein assembly machinery plays a major role in the biogenesis of its unique mitochondrial genome segregation system.
Fri Dec 01 00:00:00 UTC 2017
Clinical-scale expansion of CD34+ cord blood cells amplifies committed progenitors and rapid scid repopulation cells.
New biotechnology
Casamayor-Genescà A,Pla A,Oliver-Vila I,Pujals-Fonts N,Marín-Gallén S,Caminal M,Pujol-Autonell I,Carrascal J,Vives-Pi M,Garcia J,Vives J
132500 was used in ELISA to optimize a method to generate granulocyte lineage-committed progenitors from umbilical cord blood samples
Sat Mar 25 00:00:00 UTC 2017
Membrane skeletal association and post-translational allosteric regulation of Toxoplasma gondii GAPDH1.
Molecular microbiology
Dubey R,Staker BL,Foe IT,Bogyo M,Myler PJ,Ngô HM,Gubbels MJ
13-2500 was used in Immunoprecipitation to elucidate the crystal structure of the GAPDH1 holoenzyme in a quaternary complex to investigate its translocation following Toxoplasma gondii egress.
Wed Feb 01 00:00:00 UTC 2017
Dentin sialoprotein facilitates dental mesenchymal cell differentiation and dentin formation.
Scientific reports
Li W,Chen L,Chen Z,Wu L,Feng J,Wang F,Shoff L,Li X,Donly KJ,MacDougall M,Chen S
13-2500 was used in Western Blot to examine the mechanisms of dentin sialoprotein in controlling dental mesenchymal cell differentiation.
Wed Mar 22 00:00:00 UTC 2017
An engineered protein antagonist of K-Ras/B-Raf interaction.
Scientific reports
Kauke MJ,Traxlmayr MW,Parker JA,Kiefer JD,Knihtila R,McGee J,Verdine G,Mattos C,Wittrup KD
13-2500 was used in Western Blotting to engineer a protein scaffold with an antagonistic effect on the K-Ras/B-Raf interaction.
Wed Jul 19 00:00:00 UTC 2017
Unusual life cycle and impact on microfibril assembly of ADAMTS17, a secreted metalloprotease mutated in genetic eye disease.
Scientific reports
Hubmacher D,Schneider M,Berardinelli SJ,Takeuchi H,Willard B,Reinhardt DP,Haltiwanger RS,Apte SS
132500 was used in western blot to analyze the expression and characteristics of the secreted metalloprotease ADAMTS17 in regards to fibrillin microfibrils
Wed Feb 08 00:00:00 UTC 2017
Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse.
Proceedings of the National Academy of Sciences of the United States of America
Kanemori Y,Koga Y,Sudo M,Kang W,Kashiwabara S,Ikawa M,Hasuwa H,Nagashima K,Ishikawa Y,Ogonuki N,Ogura A,Baba T
13-2500 was used in immunocytochemistry to study regulation by pre-mRNA alternative splicing of Acrbp in the mouse by biogenesis of sperm acrosome
Tue Jun 28 00:00:00 UTC 2016
The NPC2 protein: A novel dog allergen.
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology
Khurana T,Newman-Lindsay S,Young PR,Slater JE
132500 was used in western blot to discuss dog allergens such as Can f 1
Sun May 01 00:00:00 UTC 2016
PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications.
Cell cycle (Georgetown, Tex.)
Kourtidis A,Anastasiadis PZ
13-2500 was used in western blot to characterize an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications defined by PLEKHA7
Tue Dec 13 00:00:00 UTC 2016
The levels of epithelial anchor proteins β-catenin and zona occludens-1 are altered by E7 of human papillomaviruses 5 and 8.
The Journal of general virology
Heuser S,Hufbauer M,Marx B,Tok A,Majewski S,Pfister H,Akgül B
132500 was used in western blot to show that beta-catenin and ZO-1 are targets of E7 of the oncogenic human papillomaviruses types 5 and 8
Mon Feb 01 00:00:00 UTC 2016
Nek2A phosphorylates and stabilizes SuFu: A new strategy of Gli2/Hedgehog signaling regulatory mechanism.
Cellular signalling
Wang Y,Li Y,Hu G,Huang X,Rao H,Xiong X,Luo Z,Lu Q,Luo S
13-2500 was used in western blot to research stabilization and phosphorylation of SuFu by Nek2A as a strategy for regulating signaling of Gli2/hedgehog
Thu Sep 01 00:00:00 UTC 2016
Mammalian Actin-binding Protein-1/Hip-55 Interacts with FHL2 and Negatively Regulates Cell Invasion.
The Journal of biological chemistry
Boateng LR,Bennin D,De Oliveira S,Huttenlocher A
132500 was used in western blot to report that mammalian actin-binding protein-1 and FHL2 are binding partners that regulate Rho GTPase signaling and breast cancer cell invasion
Fri Jul 01 00:00:00 UTC 2016
The non-canonical mitochondrial inner membrane presequence translocase of trypanosomatids contains two essential rhomboid-like proteins.
Nature communications
Harsman A,Oeljeklaus S,Wenger C,Huot JL,Warscheid B,Schneider A
132500 was used in immunocytochemistry and western blot to find non-canonical inner membrane protein translocation machinery in Trypanosoma brucei
Mon Dec 19 00:00:00 UTC 2016
The glucose-sensing transcription factor MLX promotes myogenesis via myokine signaling.
Genes & development
Hunt LC,Xu B,Finkelstein D,Fan Y,Carroll PA,Cheng PF,Eisenman RN,Demontis F
13-2500 was used in Chromatin immunoprecipitation to study the mechanisms of myogenesis promoted by MLX through the induction of several myokines in response to glucose.
Tue Dec 01 00:00:00 UTC 2015
The Inhibition of Heat Shock Protein 90 Facilitates the Degradation of Poly-Alanine Expanded Poly (A) Binding Protein Nuclear 1 via the Carboxyl Terminus of Heat Shock Protein 70-Interacting Protein.
PloS one
Shi C,Huang X,Zhang B,Zhu D,Luo H,Lu Q,Xiong WC,Mei L,Luo S
13-2500 was used in immunoprecipitation and western blot to discuss factors that contribute to the onset and progression of oculopharyngeal muscular dystrophy.
Tue Jun 07 00:00:00 UTC 2016
The B. subtilis Accessory Helicase PcrA Facilitates DNA Replication through Transcription Units.
PLoS genetics
Merrikh CN,Brewer BJ,Merrikh H
13-2500 was used in Chromatin immunoprecipitation to establish PcrA as an essential factor involved in mitigating transcription-replication conflicts and identify chromosomal regions where it routinely acts.
Mon Jun 01 00:00:00 UTC 2015
Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG.
Nature communications
Fischer N,Elson G,Magistrelli G,Dheilly E,Fouque N,Laurendon A,Gueneau F,Ravn U,Depoisier JF,Moine V,Raimondi S,Malinge P,Di Grazia L,Rousseau F,Poitevin Y,Calloud S,Cayatte PA,Alcoz M,Pontini G,Fagète S,Broyer L,Corbier M,Schrag D,Didelot G,Bosson N,Costes N,Cons L,Buatois V,Johnson Z,Ferlin W,Masternak K,Kosco-Vilbois M
13-2500 was used in western blot to describe a method to generate bispecific antibodies
Thu Feb 12 00:00:00 UTC 2015
Delineating the Tes Interaction Site in Zyxin and Studying Cellular Effects of Its Disruption.
PloS one
Hadzic E,Catillon M,Halavatyi A,Medves S,Van Troys M,Moes M,Baird MA,Davidson MW,Schaffner-Reckinger E,Ampe C,Friederich E
13-2500 was used in immunocytochemistry to investigate the interaction between zyxin and Tes
Mon Jun 13 00:00:00 UTC 2016
Ajuba Preferentially Binds LXRα/RXRγ Heterodimer to Enhance LXR Target Gene Expression in Liver Cells.
Molecular endocrinology (Baltimore, Md.)
Fan H,Dong W,Li Q,Zou X,Zhang Y,Wang J,Li S,Liu W,Dong Y,Sun H,Hou Z
13-2500 was used in ChIP assay and western blot to show that Ajuba is a novel coactivator for liver X receptors.
Sun Nov 01 00:00:00 UTC 2015
Novel components of the Toxoplasma inner membrane complex revealed by BioID.
mBio
Chen AL,Kim EW,Toh JY,Vashisht AA,Rashoff AQ,Van C,Huang AS,Moon AS,Bell HN,Bentolila LA,Wohlschlegel JA,Bradley PJ
13-2500 was used in immunocytochemistry to use BioID to find novel components of the Toxoplasm inner membrane complex
Tue Feb 17 00:00:00 UTC 2015
Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation.
Nature communications
Asghar A,Lajeunesse A,Dulla K,Combes G,Thebault P,Nigg EA,Elowe S
13-2500 was used in immunocytochemistry and western blot to elucidate the regulation and dynamics of H2A-T120 phosphorylation by Bub1
Thu Sep 24 00:00:00 UTC 2015
RON5 is critical for organization and function of the Toxoplasma moving junction complex.
PLoS pathogens
Beck JR,Chen AL,Kim EW,Bradley PJ
13-2500 was used in western blot to determine the role of RON5 as a critical component for function and organization of the Toxoplasma moving junction complex
Sat Mar 01 00:00:00 UTC 2014
Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation.
Journal of cell science
Chandra S,Priyadarshini R,Madhavan V,Tikoo S,Hussain M,Mudgal R,Modi P,Srivastava V,Sengupta S
13-2500 was used in western blot to study the promotion of c-Myc degradation by BLM helicase and its effect on tumor initiation
Thu Aug 15 00:00:00 UTC 2013
Rac1 acts in conjunction with Nedd4 and dishevelled-1 to promote maturation of cell-cell contacts.
Journal of cell science
Nethe M,de Kreuk BJ,Tauriello DV,Anthony EC,Snoek B,Stumpel T,Salinas PC,Maurice MM,Geerts D,Deelder AM,Hensbergen PJ,Hordijk PL
13-2500 was used in Western Blotting to investigate the interactions between Rac1, Nedd4, and dishevelled-1 in the maturation of cell-cell junctions.
Sun Jul 15 00:00:00 UTC 2012
Activation of myeloid cell-specific adhesion class G protein-coupled receptor EMR2 via ligation-induced translocation and interaction of receptor subunits in lipid raft microdomains.
Molecular and cellular biology
Huang YS,Chiang NY,Hu CH,Hsiao CC,Cheng KF,Tsai WP,Yona S,Stacey M,Gordon S,Chang GW,Lin HH
Sun Apr 01 00:00:00 UTC 2012
Activation of myeloid cell-specific adhesion class G protein-coupled receptor EMR2 via ligation-induced translocation and interaction of receptor subunits in lipid raft microdomains.
Molecular and cellular biology
Huang YS,Chiang NY,Hu CH,Hsiao CC,Cheng KF,Tsai WP,Yona S,Stacey M,Gordon S,Chang GW,Lin HH
Sun Apr 01 00:00:00 UTC 2012
Array-based comparative genomic hybridization identifies CDK4 and FOXM1 alterations as independent predictors of survival in malignant peripheral nerve sheath tumor.
Clinical cancer research : an official journal of the American Association for Cancer Research
Yu J,Deshmukh H,Payton JE,Dunham C,Scheithauer BW,Tihan T,Prayson RA,Guha A,Bridge JA,Ferner RE,Lindberg GM,Gutmann RJ,Emnett RJ,Salavaggione L,Gutmann DH,Nagarajan R,Watson MA,Perry A
13-2500 was used in immunohistochemistry - paraffin section to identify survival-associated genomic biomarkers for malignant peripheral nerve sheath tumors.
Fri Apr 01 00:00:00 UTC 2011
Ribosomal protein L11 recruits miR-24/miRISC to repress c-Myc expression in response to ribosomal stress.
Molecular and cellular biology
Challagundla KB,Sun XX,Zhang X,DeVine T,Zhang Q,Sears RC,Dai MS
Sat Oct 01 00:00:00 UTC 2011
Ribosomal protein L11 recruits miR-24/miRISC to repress c-Myc expression in response to ribosomal stress.
Molecular and cellular biology
Challagundla KB,Sun XX,Zhang X,DeVine T,Zhang Q,Sears RC,Dai MS
Sat Oct 01 00:00:00 UTC 2011
ADAMTS9 is a cell-autonomously acting, anti-angiogenic metalloprotease expressed by microvascular endothelial cells.
The American journal of pathology
Koo BH,Coe DM,Dixon LJ,Somerville RP,Nelson CM,Wang LW,Young ME,Lindner DJ,Apte SS
13-2500 was used in western blot to characterize angiogenesis defects in ADAMTS9+/- mice
Mon Mar 01 00:00:00 UTC 2010
Aurora-A phosphorylates, activates, and relocalizes the small GTPase RalA.
Molecular and cellular biology
Lim KH,Brady DC,Kashatus DF,Ancrile BB,Der CJ,Cox AD,Counter CM
13-2500 was used in immunocytochemistry and western blot to show that Aurora-A in conjunction with oncogenic Ras enhances transformed cell growth
Fri Jan 01 00:00:00 UTC 2010
Ribosomal protein L11 associates with c-Myc at 5 S rRNA and tRNA genes and regulates their expression.
The Journal of biological chemistry
Dai MS,Sun XX,Lu H
13-2500 was used in ChIP assay and western blot to investigate the role of L11 in the regulation of c-Myc-driven transcription
Fri Apr 23 00:00:00 UTC 2010
Functional equivalence of the clathrin heavy chains CHC17 and CHC22 in endocytosis and mitosis.
Journal of cell science
Hood FE,Royle SJ
13-2500 was used in immunoprecipitation to compare clathrin paralogues in functional assays for endocytosis and mitosis
Wed Jul 01 00:00:00 UTC 2009
An adaptor role for cytoplasmic Sam68 in modulating Src activity during cell polarization.
Molecular and cellular biology
Huot ME,Brown CM,Lamarche-Vane N,Richard S
13-2500 was used in immunocytochemistry to demonstrate that Sam68-deficient cells exhibit loss of cell polarity and cell migration
Wed Apr 01 00:00:00 UTC 2009
Classic and desmoplastic medulloblastoma: complete case reports and characterizations of two new cell lines.
Neuropathology : official journal of the Japanese Society of Neuropathology
Holthouse DJ,Dallas PB,Ford J,Fabian V,Murch AR,Watson M,Wong G,Bertram C,Egli S,Baker DL,Kees UR
13-2500 was used in western blot to report on two cases of classic and desmoplastic medulloblastoma and the characterization of two new cell lines
Sat Aug 01 00:00:00 UTC 2009
Regulatory mechanism of matrix metalloprotease-2 enzymatic activity by factor Xa and thrombin.
The Journal of biological chemistry
Koo BH,Park MY,Jeon OH,Kim DS
13-2500 was used in western blot to discuss how factor Xa and thrombin regulate MMP-2 enzymatic activity
Fri Aug 28 00:00:00 UTC 2009
Immunohistochemical analysis of the progression of flat and papillary preneoplastic lesions in intrahepatic cholangiocarcinogenesis in hepatolithiasis.
Liver international : official journal of the International Association for the Study of the Liver
Itatsu K,Zen Y,Ohira S,Ishikawa A,Sato Y,Harada K,Ikeda H,Sasaki M,Nimura Y,Nakanuma Y
13-2500 was used in immunohistochemistry - paraffin section to analyze the progression of flat and papillary preneoplastic lesions in intrahepatic cholangiocarcinogenesis in hepatolithiasis
Thu Nov 01 00:00:00 UTC 2007
Analysis of a Shc family adaptor protein, ShcD/Shc4, that associates with muscle-specific kinase.
Molecular and cellular biology
Jones N,Hardy WR,Friese MB,Jorgensen C,Smith MJ,Woody NM,Burden SJ,Pawson T
13-2500 was used in western blot to study ShcD/Shc4 interactions with the MuSK receptor
Sun Jul 01 00:00:00 UTC 2007
Mutations in PDLIM3 and MYOZ1 encoding myocyte Z line proteins are infrequently found in idiopathic dilated cardiomyopathy.
Molecular genetics and metabolism
Arola AM,Sanchez X,Murphy RT,Hasle E,Li H,Elliott PM,McKenna WJ,Towbin JA,Bowles NE
13-2500 was used in immunocytochemistry to investigate the contribution of PDLIM3 and MYOZ1 to dilated cardiomyopathy
Sun Apr 01 00:00:00 UTC 2007
Kinetic mechanism of the fastest motor protein, Chara myosin.
The Journal of biological chemistry
Ito K,Ikebe M,Kashiyama T,Mogami T,Kon T,Yamamoto K
13-2500 was used to perform a kinetic analysis of a recombinant motor domain of Chara myosin
Fri Jul 06 00:00:00 UTC 2007
Regulation of ADAMTS9 secretion and enzymatic activity by its propeptide.
The Journal of biological chemistry
Koo BH,Longpré JM,Somerville RP,Alexander JP,Leduc R,Apte SS
13-2500 was used in western blot to examine the role of the ADAMTS9 propeptide in regulating its secretion and proteolytic activity
Fri Jun 01 00:00:00 UTC 2007
The prolyl isomerase Pin1 affects Che-1 stability in response to apoptotic DNA damage.
The Journal of biological chemistry
De Nicola F,Bruno T,Iezzi S,Di Padova M,Floridi A,Passananti C,Del Sal G,Fanciulli M
13-2500 was used in immunoprecipitation and western blot to show that Che-1 is a new Pin1 and HDM2 target and has a role in the cellular response to DNA damage
Fri Jul 06 00:00:00 UTC 2007
AP-1 differentially expressed proteins Krp1 and fibronectin cooperatively enhance Rho-ROCK-independent mesenchymal invasion by altering the function, localization, and activity of nondifferentially expressed proteins.
Molecular and cellular biology
Spence HJ,McGarry L,Chew CS,Carragher NO,Scott-Carragher LA,Yuan Z,Croft DR,Olson MF,Frame M,Ozanne BW
13-2500 was used in western blot determine the role of Krp1 in v-Fos-transformed rat fibroblast cells
Wed Feb 01 00:00:00 UTC 2006
The binding of DYNLL2 to myosin Va requires alternatively spliced exon B and stabilizes a portion of the myosin's coiled-coil domain.
Biochemistry
Wagner W,Fodor E,Ginsburg A,Hammer JA
13-2500 was used in western blot to map the binding site for DYNLL2 within the myosin Va heavy chain
Tue Sep 26 00:00:00 UTC 2006
Collagen triple helix repeat containing 1, a novel secreted protein in injured and diseased arteries, inhibits collagen expression and promotes cell migration.
Circulation research
Pyagay P,Heroult M,Wang Q,Lehnert W,Belden J,Liaw L,Friesel RE,Lindner V
13-2500 was used in western blot to investigate injury-induced collagen triple helix repeat containing 1 expression
Fri Feb 04 00:00:00 UTC 2005
Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin.
The EMBO journal
Soosairajah J,Maiti S,Wiggan O,Sarmiere P,Moussi N,Sarcevic B,Sampath R,Bamburg JR,Bernard O
13-2500 was used in immunoprecipitation and western blot to identify and characterize an ADF/cofilin phosphoregulatory complex
Wed Feb 09 00:00:00 UTC 2005
Akt phosphorylates Tal1 oncoprotein and inhibits its repressor activity.
Cancer research
Palamarchuk A,Efanov A,Maximov V,Aqeilan RI,Croce CM,Pekarsky Y
Wed Jun 01 00:00:00 UTC 2005
Akt phosphorylates Tal1 oncoprotein and inhibits its repressor activity.
Cancer research
Palamarchuk A,Efanov A,Maximov V,Aqeilan RI,Croce CM,Pekarsky Y
Wed Jun 01 00:00:00 UTC 2005
Akt phosphorylates Tal1 oncoprotein and inhibits its repressor activity.
Cancer research
Palamarchuk A,Efanov A,Maximov V,Aqeilan RI,Croce CM,Pekarsky Y
Wed Jun 01 00:00:00 UTC 2005
Akt phosphorylates Tal1 oncoprotein and inhibits its repressor activity.
Cancer research
Palamarchuk A,Efanov A,Maximov V,Aqeilan RI,Croce CM,Pekarsky Y
Wed Jun 01 00:00:00 UTC 2005
WW domain-containing proteins, WWOX and YAP, compete for interaction with ErbB-4 and modulate its transcriptional function.
Cancer research
Aqeilan RI,Donati V,Palamarchuk A,Trapasso F,Kaou M,Pekarsky Y,Sudol M,Croce CM
Mon Aug 01 00:00:00 UTC 2005
WW domain-containing proteins, WWOX and YAP, compete for interaction with ErbB-4 and modulate its transcriptional function.
Cancer research
Aqeilan RI,Donati V,Palamarchuk A,Trapasso F,Kaou M,Pekarsky Y,Sudol M,Croce CM
Mon Aug 01 00:00:00 UTC 2005
Function and regulation of Tumbleweed (RacGAP50C) in neuroblast proliferation and neuronal morphogenesis.
Proceedings of the National Academy of Sciences of the United States of America
Goldstein AY,Jan YN,Luo L
13-2500 was used in immunohistochemistry to report that Tumbleweed, Pavarotti, and their association are required for neuroblast proliferation in Drosophila
Tue Mar 08 00:00:00 UTC 2005
A role for Ubc9 in tumorigenesis.
Oncogene
Mo YY,Yu Y,Theodosiou E,Ee PL,Beck WT
Thu Apr 14 00:00:00 UTC 2005
A role for Ubc9 in tumorigenesis.
Oncogene
Mo YY,Yu Y,Theodosiou E,Ee PL,Beck WT
Thu Apr 14 00:00:00 UTC 2005
Targeting of Arf-1 to the early Golgi by membrin, an ER-Golgi SNARE.
The Journal of cell biology
Honda A,Al-Awar OS,Hay JC,Donaldson JG
Mon Mar 28 00:00:00 UTC 2005
Proteolytic maturation and activation of autotaxin (NPP2), a secreted metastasis-enhancing lysophospholipase D.
Journal of cell science
Jansen S,Stefan C,Creemers JW,Waelkens E,Van Eynde A,Stalmans W,Bollen M
13-2500 was used in immunocytochemistry to investigate the maturation of autotaxin
Fri Jul 15 00:00:00 UTC 2005
Distinct roles of EGF repeats for the Notch signaling system.
Experimental cell research
Sakamoto K,Chao WS,Katsube K,Yamaguchi A
13-2500 was used in western blot to report that Notch1 forms homodimers via its EGF motifs
Sat Jan 15 00:00:00 UTC 2005
Characterization of the human GARP (Golgi associated retrograde protein) complex.
Experimental cell research
Liewen H,Meinhold-Heerlein I,Oliveira V,Schwarzenbacher R,Luo G,Wadle A,Jung M,Pfreundschuh M,Stenner-Liewen F
13-2500 was used in immunoprecipitation and western blot to identify and characterize a Vps fifty-three tethering factor complex
Sun May 15 00:00:00 UTC 2005
Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential interaction partners of testicular germ cell-specific protein GGN1.
FEBS letters
Zhang J,Wang Y,Zhou Y,Cao Z,Huang P,Lu B
13-2500 was used in immunocytochemistry, immunoprecipitation, and western blot to investigate the function of gametogenetin protein 1
Mon Jan 17 00:00:00 UTC 2005
Functional properties of the p33 and p55 domains of the Helicobacter pylori vacuolating cytotoxin.
The Journal of biological chemistry
Torres VJ,Ivie SE,McClain MS,Cover TL
13-2500 was used in western blot to characterize vacuolating cytotoxin from Helicobacter pylori
Fri Jun 03 00:00:00 UTC 2005
Trapping prion protein in the endoplasmic reticulum impairs PrPC maturation and prevents PrPSc accumulation.
The Journal of biological chemistry
Cardinale A,Filesi I,Vetrugno V,Pocchiari M,Sy MS,Biocca S
13-2500 was used in immunocytochemistry and western blot to determine where prion protein is converted into the abnormal scrapie isoform intracellularly
Fri Jan 07 00:00:00 UTC 2005
HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin.
The Journal of biological chemistry
Iwata A,Riley BE,Johnston JA,Kopito RR
13-2500 was used in western blot to report that autophagy is induced by impaired ubiquitin proteasome system activity
Fri Dec 02 00:00:00 UTC 2005
A homologue of Cdk8 is required for spore cell differentiation in Dictyostelium.
Developmental biology
Lin HH,Khosla M,Huang HJ,Hsu DW,Michaelis C,Weeks G,Pears C
13-2500 was used in immunocytochemistry, immunoprecipitation, and western blot to propose a direct role for DdCdk8 in spore formation
Thu Jul 01 00:00:00 UTC 2004
Isoforms of the polarity protein par6 have distinct functions.
The Journal of biological chemistry
Gao L,Macara IG
Fri Oct 01 00:00:00 UTC 2004
Identification of a role for beta-catenin in the establishment of a bipolar mitotic spindle.
The Journal of biological chemistry
Kaplan DD,Meigs TE,Kelly P,Casey PJ
Fri Mar 19 00:00:00 UTC 2004
Identification of a role for beta-catenin in the establishment of a bipolar mitotic spindle.
The Journal of biological chemistry
Kaplan DD,Meigs TE,Kelly P,Casey PJ
Fri Mar 19 00:00:00 UTC 2004
The E box motif CAGGTG enhances somatic hypermutation without enhancing transcription.
Immunity
Michael N,Shen HM,Longerich S,Kim N,Longacre A,Storb U
13-2500 was used in Western Blotting to find that the CAGGTG motif enhances somatic hypermutation without enhancing transcription.
Fri Aug 01 00:00:00 UTC 2003
Expression of c-erbB2, p53, Bcl-2, Bax, c-myc and Ki-67 in apocrine metaplasia and apocrine change within sclerosing adenosis of the breast.
Virchows Archiv : an international journal of pathology
Selim AG,El-Ayat G,Wells CA
13-2500 was used in immunohistochemistry - paraffin section to examine the similarity between apocrine cells and high-grade ductal carcinoma
Fri Nov 01 00:00:00 UTC 2002
Phosphatidylinositol 4,5-bisphosphate and Arf6-regulated membrane traffic.
The Journal of cell biology
Brown FD,Rozelle AL,Yin HL,Balla T,Donaldson JG
13-2500 was used in immunocytochemistry to show that PIP 5-kinase activity and PIP2 turnover are critical for trafficking through the Arf6 PM-endosomal recycling pathway
Mon Sep 03 00:00:00 UTC 2001
Agonist-promoted internalization of a ternary complex between calcitonin receptor-like receptor, receptor activity-modifying protein 1 (RAMP1), and beta-arrestin.
The Journal of biological chemistry
Hilairet S,Bélanger C,Bertrand J,Laperrière A,Foord SM,Bouvier M
13-2500 was used in flow cytometry, immunocytochemistry, immunoprecipitation, and western blot to study oligomerization and localization of the calcitonin receptor-like receptor
Fri Nov 09 00:00:00 UTC 2001
Involvement of phospholipase D in sphingosine 1-phosphate-induced activation of phosphatidylinositol 3-kinase and Akt in Chinese hamster ovary cells overexpressing EDG3.
The Journal of biological chemistry
Banno Y,Takuwa Y,Akao Y,Okamoto H,Osawa Y,Naganawa T,Nakashima S,Suh PG,Nozawa Y
13-2500 was used in immunoprecipitation to report that phospholipase D contributes to the activation of PI3K and Akt stimulation of EDG3 receptor
Fri Sep 21 00:00:00 UTC 2001
Leucine zipper-mediated homodimerization of the p21-activated kinase-interacting factor, beta Pix. Implication for a role in cytoskeletal reorganization.
The Journal of biological chemistry
Kim S,Lee SH,Park D
13-2500 was used in immunoprecipitation to show that the leucine zipper domain mediates the formation of betaPix-a homodimers
Fri Apr 06 00:00:00 UTC 2001
Transendothelial migration of colon carcinoma cells requires expression of E-selectin by endothelial cells and activation of stress-activated protein kinase-2 (SAPK2/p38) in the tumor cells.
The Journal of biological chemistry
Laferriere J,Houle F,Taher MM,Valerie K,Huot J
13-2500 was used in western blot to study the roles of E-selectin and of stress-activated protein kinase-2 in modulating endothelial adhesion and transendothelial migration
Fri Sep 07 00:00:00 UTC 2001
PYK2 as a mediator of endothelin-1/G alpha 11 signaling to GLUT4 glucose transporters.
The Journal of biological chemistry
Park JG,Bose A,Leszyk J,Czech MP
13-2500 was used in immunocytochemistry to identify proteins selectively tyrosine-phosphorylated in response to ET-1 but not insulin
Fri Dec 21 00:00:00 UTC 2001
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Molecular and cellular biology
Evan GI,Lewis GK,Ramsay G,Bishop JM
13-2500 was used in western blot to describe antibodies that can be used to quantitate p62c-myc in cells
Sun Dec 01 00:00:00 UTC 1985
