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            • Primary Antibodies ›
            • RANKL (RANK Ligand) Antibodies

            Invitrogen

            CD254 (RANK Ligand) Monoclonal Antibody (IK22/5), Biotin, eBioscience™

            5个已发表图片
            7 篇参考文献
            查看其他49个RANKL (RANK Ligand)抗体
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            Cite CD254 (RANK Ligand) Monoclonal Antibody (IK22/5), Biotin, eBioscience™

            • 抗体检测数据 (1)
            • 已发表图片 (5)
            Application
            CD254 (RANK Ligand) Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            图: 1 / 6

            CD254 (RANK Ligand) Antibody (13-5952-82) in Flow

            Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.125 µg of Biotin Rat IgG2a Isotype Control (Product # 13-4321-82) (open histogram) or 0.125 µg of Anti-Mouse CD254 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
            查看产品

            CD254 (RANK Ligand) Antibody in Flow Cytometry (Flow)
            CD254 (RANK Ligand) Antibody in Immunocytochemistry (ICC/IF)
            CD254 (RANK Ligand) Antibody in Immunocytochemistry (ICC/IF)
            CD254 (RANK Ligand) Antibody in Immunohistochemistry (IHC)
            CD254 (RANK Ligand) Antibody in Flow Cytometry (Flow)
            CD254 (RANK Ligand) Antibody in Immunomicroscopy (IM)

            产品信息

            13-5952-82

            应用
            建议稀释比
            已发表文章

            免疫组化 (IHC)

            -
            查看2篇已发表文章 2篇已发表文章

            免疫细胞化学 (ICC/IF)

            -
            查看2篇已发表文章 2篇已发表文章

            流式细胞分析 (Flow)

            0.25 µg/test
            查看2篇已发表文章 2篇已发表文章

            免疫电镜技术 (IM)

            -
            查看1篇已发表文章 1篇已发表文章
            产品规格

            种属反应

            Mouse

            已发表种属

            Mouse

            宿主/亚型

            Rat / IgG2a, kappa

            推荐的同型对照

            Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

            分类

            Monoclonal

            类型

            Antibody

            克隆号

            IK22/5

            偶联物

            Biotin
            • Unconjugated
            • Functional Grade
            • PE 
            • Request custom conjugation

            形式

            Liquid

            浓度

            0.5 mg/mL

            纯化类型

            Affinity chromatography

            保存液

            PBS, pH 7.2

            内含物

            0.09% sodium azide

            保存条件

            4°C, store in dark, DO NOT FREEZE!

            运输条件

            Ambient (domestic); Wet ice (international)

            RRID

            AB_466813

            产品详细信息

            Description: The IK22/5 monoclonal antibody reacts with mouse CD254 also known as TRANCE (TNF-related activation-induced cytokine receptor) and RANKL (receptor activator of NF-kappa B ligand) and OPGL (Osteoprotegerin Ligand). TRANCE is a type II membrane protein with predicted molecular weight of 35 kDa. Its extracellular domain is most closely related to TRAIL, FasL and TNF. TRANCE mRNA is upregulated by TCR stimulation and is expressed abundantly by mouse T cells not B cells in mouse thymus and lymph nodes. TRANCE-deficient mouse lacks osteoclasts. In addition, TRANCE has been shown to be involved in the interactions between T-dendritic and T-B cells. It was also found to be critical in osteoclast differentiation. The TRANCE-RANK interaction regulates the expression of the anti-apoptotic molecule Bcl-xL.

            Applications Reported: This IK22/5 antibody has been reported for use in flow cytometric analysis.

            Applications Tested: The IK22/5 antibody has been tested by flow cytometric analysis of mouse CD254 transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Filtration: 0.2 µm post-manufacturing filtered.

            靶标信息

            This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

            仅用于科研。不用于诊断过程。未经明确授权不得转售。

            生物信息学

            蛋白别名: CD254; H-RANK-L; M-Rank-L; ODF; OPG ligand; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; rank-l; RANKL; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; srankl; TNF-related activation-induced cytokine; TRANCE; Tumor necrosis factor ligand superfamily member 11; tumor necrosis factor-related activation-induced cytokine

            查看更多 收起

            基因别名: Ly109l; ODF; OPG; OPGL; RANKL; Tnfsf11; Trance

            查看更多 收起

            UniProt ID: (Mouse) O35235

            查看更多 收起

            Entrez Gene ID: (Mouse) 21943

            查看更多 收起

            功能

            cytokine activity tumor necrosis factor receptor binding protein binding tumor necrosis factor receptor superfamily binding

            参与通路

            ossification osteoclast proliferation monocyte chemotaxis immune response activation of JUN kinase activity multicellular organism development organ morphogenesis cytokine-mediated signaling pathway cell differentiation osteoclast differentiation tumor necrosis factor-mediated signaling pathway mammary gland epithelial cell proliferation positive regulation of homotypic cell-cell adhesion paracrine signaling positive regulation of phosphorylation positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAP kinase activity bone resorption regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of bone resorption positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade lymph node development positive regulation of T cell activation positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity protein homooligomerization positive regulation of corticotropin-releasing hormone secretion positive regulation of protein kinase B signaling calcium ion homeostasis mammary gland alveolus development ERK1 and ERK2 cascade positive regulation of fever generation by positive regulation of prostaglandin secretion TNFSF11-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade via TNFSF11-mediated signaling positive regulation of intracellular signal transduction positive regulation of osteoclast development
            It has to be done as per old AB suggested Products section.
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