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Invitrogen

VEGF Receptor 3 Monoclonal Antibody (AFL4), eBioscience™

Advanced Verification

11 Published Figures

23 References

View all (35) VEGF Receptor 3 antibodies

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Catalog # 14-5988-85

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Datasheet

Protocols

Questions & Answers

Published Figures (11)
Advanced Verification (1)

Application

VEGF Receptor 3 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 12

VEGF Receptor 3 Antibody (14-5988-85) in IHC

Life science alliance 2022 - Figure S5. Increased Vegfr3 expression induced by Tbx1 Cre activation of TgVegfr3 is revealed by qRT-PCR but not by immunofluorescence. (A, B, C, D, E, F, G, H, I, J, K, L) Representative coronal brain sections of Tbx1 Cre/ + (A, B, C, D, E, F) and TgVegfr3; Tbx1 Cre/ + (G, H, I, J, K, L) embryos (E18.5) stained for VEGFR3 (red), VEGFR2 (green), and GFP ( Tbx1 cell fate, white). The cartoon shows the positi... View More

VEGF Receptor 3 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

VEGF Receptor 3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

VEGF Receptor 3 Antibody in Western Blot (WB)

VEGF Receptor 3 Antibody in Flow Cytometry (Flow)

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Product Details

14-5988-85

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-Dependent

View 1 publication 1 publication

Neutralization (Neu)

Assay-Dependent

Functional Assay (Functional)

Assay-Dependent

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

AFL4

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_467796

Product Specific Information

Description: The AFL4 monoclonal antibody reacts with the mouse VEGF receptor-3, also known as Flt-4. This 195 kDa molecule was identified as an endothelial-specific member of the receptor tyrosine kinase (RTK) family. During early embryogenesis all endothelial cells express VEGFR-3, while in the adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. However, VEGFR-3 expression is induced in the adult tissue upon tumor implementation suggesting an important role for this receptor in the tumor angiogenesis. VEGF-C and VEGF-D bind to and activate VEGFR-3. AFL4 is an antagonist mAb.

Applications Reported: The AFL4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking of ligand binding. (Please use Functional Grade purified AFL4, Product # 16-5988, in functional assays).

Applications Tested: The AFL4 antibody has been tested by flow cytometric analysis of in vitro differentiated mouse endothelial cells. In brief, mouse ES cells were incubated on collagen IV matrix for 4 days and subsequently stimulated with VEGF under serum free conditions to induce further differentiation. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Vascular endothelial growth factor is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues. Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specificl ymphatic endothelial receptor VEGFR-3. In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos. Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation in the pericardial cavity and cardiovascular failure at embryonic day 9.5. Thus, VEGFR3 has an essential role in the development of theembryonic cardiovascular system before the emergence of the lymphatic vessels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lipid droplet degradation by autophagy connects mitochondria metabolism to Prox1-driven expression of lymphatic genes and lymphangiogenesis.

Nature communications

Meçe O,Houbaert D,Sassano ML,Durré T,Maes H,Schaaf M,More S,Ganne M,García-Caballero M,Borri M,Verhoeven J,Agrawal M,Jacobs K,Bergers G,Blacher S,Ghesquière B,Dewerchin M,Swinnen JV,Vinckier S,Soengas MS,Carmeliet P,Noël A,Agostinis P

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 14-5988-82) in Immunohistochemistry

Thu May 19 00:00:00 UTC 2022

VEGFR3 modulates brain microvessel branching in a mouse model of 22q11.2 deletion syndrome.

Life science alliance

Cioffi S,Flore G,Martucciello S,Bilio M,Turturo MG,Illingworth E

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 14-5988-82) in Immunohistochemistry

Mon Oct 10 00:00:00 UTC 2022

Comparative analysis of cell lineage differentiation during hepatogenesis in humans and mice at the single-cell transcriptome level.

Cell research

Wang X,Yang L,Wang YC,Xu ZR,Feng Y,Zhang J,Wang Y,Xu CR

14-5988 was used in Immunohistochemistry-immunofluorescence to study cell lineage differentiation during hepatogenesis using single-cell RNA sequencing of whole human and mouse fetal livers throughout development.

Tue Dec 01 00:00:00 UTC 2020

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 14-5988-82) in Flow Cytometry

Thu Dec 01 00:00:00 UTC 2016

VEGF-D promotes pulmonary oedema in hyperoxic acute lung injury.

The Journal of pathology

Sato T,Paquet-Fifield S,Harris NC,Roufail S,Turner DJ,Yuan Y,Zhang YF,Fox SB,Hibbs ML,Wilkinson-Berka JL,Williams RA,Stacker SA,Sly PD,Achen MG

14-5988 was used in Immunohistochemistry to demonstrate that VEGF-D and its receptors were co-localized on blood vessels in clinical samples of human lungs exposed to hyperoxia.

Wed Jun 01 00:00:00 UTC 2016

VEGF-D promotes pulmonary oedema in hyperoxic acute lung injury.

The Journal of pathology

Sato T,Paquet-Fifield S,Harris NC,Roufail S,Turner DJ,Yuan Y,Zhang YF,Fox SB,Hibbs ML,Wilkinson-Berka JL,Williams RA,Stacker SA,Sly PD,Achen MG

14-5988 was used in Immunohistochemistry to demonstrate that VEGF-D and its receptors were co-localized on blood vessels in clinical samples of human lungs exposed to hyperoxia.

Wed Jun 01 00:00:00 UTC 2016

A Selenium Containing Inhibitor for the Treatment of Hepatocellular Cancer.

Pharmaceuticals (Basel, Switzerland)

Tagaram HR,Desai D,Li G,Liu D,Rountree CB,Gowda K,Berg A,Amin S,Staveley-O'Carroll KF,Kimchi ET

Thu Mar 24 00:00:00 UTC 2016

A Selenium Containing Inhibitor for the Treatment of Hepatocellular Cancer.

Pharmaceuticals (Basel, Switzerland)

Tagaram HR,Desai D,Li G,Liu D,Rountree CB,Gowda K,Berg A,Amin S,Staveley-O'Carroll KF,Kimchi ET

Thu Mar 24 00:00:00 UTC 2016

G-Protein-Coupled Receptor-2-Interacting Protein-1 Controls Stalk Cell Fate by Inhibiting Delta-like 4-Notch1 Signaling.

Cell reports

Majumder S,Zhu G,Xu X,Senchanthisai S,Jiang D,Liu H,Xue C,Wang X,Coia H,Cui Z,Smolock EM,Libby RT,Berk BC,Pang J

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 14-5988-82) in Western Blot

Tue Dec 06 00:00:00 UTC 2016

Vascular endothelial growth factor c/vascular endothelial growth factor receptor 3 signaling regulates chemokine gradients and lymphocyte migration from tissues to lymphatics.

Transplantation

Iwami D,Brinkman CC,Bromberg JS

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 14-5988-82) in Flow Cytometry

Wed Apr 01 00:00:00 UTC 2015

Ephrin-B2 controls PDGFRβ internalization and signaling.

Genes & development

Nakayama A,Nakayama M,Turner CJ,Höing S,Lepore JJ,Adams RH

14-5988 was used in Western Blotting to show that ephrin-B2 is an important regulator of PDGFRβ endocytosis, acting as a molecular switch controlling the downstream signaling activity of this receptor in mural cells.

Sun Dec 01 00:00:00 UTC 2013

Vascular endothelial growth factor receptor-2 promotes the development of the lymphatic vasculature.

PloS one

Dellinger MT,Meadows SM,Wynne K,Cleaver O,Brekken RA

14-5988 was used in Immunohistochemistry to show that the proper development of the lymphatic vasculature requires VEGFR2 expression by lymphatic endothelium.

Tue Apr 08 00:00:00 UTC 2014

RASA1 maintains the lymphatic vasculature in a quiescent functional state in mice.

The Journal of clinical investigation

Lapinski PE,Kwon S,Lubeck BA,Wilkinson JE,Srinivasan RS,Sevick-Muraca E,King PD

14-5988 was used in Immunofluorescence to reveal a role for RASA1, a Ras GTPase-activating protein, as a physiological negative regulator of lymphatic endothelial cell growth.

Wed Feb 01 00:00:00 UTC 2012

Kidins220/ARMS mediates the integration of the neurotrophin and VEGF pathways in the vascular and nervous systems.

Cell death and differentiation

Cesca F,Yabe A,Spencer-Dene B,Scholz-Starke J,Medrihan L,Maden CH,Gerhardt H,Orriss IR,Baldelli P,Al-Qatari M,Koltzenburg M,Adams RH,Benfenati F,Schiavo G

14-5988 was used in Immunoprecipitation to indicate that Kidins220 mediates the integration of several growth factor receptor pathways during development.

Wed Feb 01 00:00:00 UTC 2012

Kidins220/ARMS mediates the integration of the neurotrophin and VEGF pathways in the vascular and nervous systems.

Cell death and differentiation

Cesca F,Yabe A,Spencer-Dene B,Scholz-Starke J,Medrihan L,Maden CH,Gerhardt H,Orriss IR,Baldelli P,Al-Qatari M,Koltzenburg M,Adams RH,Benfenati F,Schiavo G

14-5988 was used in Immunoprecipitation to indicate that Kidins220 mediates the integration of several growth factor receptor pathways during development.

Wed Feb 01 00:00:00 UTC 2012

Tumor-expressed inducible nitric oxide synthase controls induction of functional myeloid-derived suppressor cells through modulation of vascular endothelial growth factor release.

Journal of immunology (Baltimore, Md. : 1950)

Jayaraman P,Parikh F,Lopez-Rivera E,Hailemichael Y,Clark A,Ma G,Cannan D,Ramacher M,Kato M,Overwijk WW,Chen SH,Umansky VY,Sikora AG

14-5988 was used in Flow cytometry/Cell sorting to suggest a critical role for tumor-expressed inducible NO synthase in recruitment and induction of functional myeloid-derived suppressor cells.

Fri Jun 01 00:00:00 UTC 2012

Fbxw7 controls angiogenesis by regulating endothelial Notch activity.

PloS one

Izumi N,Helker C,Ehling M,Behrens A,Herzog W,Adams RH

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 14-5988-82) in Immunofluorescence

Tue Apr 02 00:00:00 UTC 2013

Fbxw7 controls angiogenesis by regulating endothelial Notch activity.

PloS one

Izumi N,Helker C,Ehling M,Behrens A,Herzog W,Adams RH

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 14-5988-82) in Immunofluorescence

Tue Apr 02 00:00:00 UTC 2013

VEGFR-3 ligand-binding and kinase activity are required for lymphangiogenesis but not for angiogenesis.

Cell research

Zhang L,Zhou F,Han W,Shen B,Luo J,Shibuya M,He Y

14-5988 was used in Western Blotting to indicate that signaling mediated via VEGFR-3 activation by its cognate ligands (VEGF-C/-D) is not required for angiogenesis.

Wed Dec 01 00:00:00 UTC 2010

Effective suppression of vascular network formation by combination of antibodies blocking VEGFR ligand binding and receptor dimerization.

Cancer cell

Tvorogov D,Anisimov A,Zheng W,Leppänen VM,Tammela T,Laurinavicius S,Holnthoner W,Heloterä H,Holopainen T,Jeltsch M,Kalkkinen N,Lankinen H,Ojala PM,Alitalo K

14-5988 was used in Western Blotting to show that combined use of antibodies blocking ligand binding and receptor dimerization improves VEGFR inhibition and results in stronger inhibition of endothelial sprouting and vascular network formation in vivo.

Tue Dec 14 00:00:00 UTC 2010

Embryonic vascular endothelial cells are malleable to reprogramming via Prox1 to a lymphatic gene signature.

BMC developmental biology

Kim H,Nguyen VP,Petrova TV,Cruz M,Alitalo K,Dumont DJ

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 14-5988-82) in Immunohistochemistry

Mon Jun 28 00:00:00 UTC 2010

Embryonic vascular endothelial cells are malleable to reprogramming via Prox1 to a lymphatic gene signature.

BMC developmental biology

Kim H,Nguyen VP,Petrova TV,Cruz M,Alitalo K,Dumont DJ

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 14-5988-82) in Immunohistochemistry

Mon Jun 28 00:00:00 UTC 2010

Targeted delivery of NK4 to multiple lung tumors by bone marrow-derived mesenchymal stem cells.

Cancer gene therapy

Kanehira M,Xin H,Hoshino K,Maemondo M,Mizuguchi H,Hayakawa T,Matsumoto K,Nakamura T,Nukiwa T,Saijo Y

14-5988 was used in Immunohistochemistry to indicate that mesenchymal stem cells can serve as a vehicle of gene therapy for targeting multiple lung metastatic tumours.

Thu Nov 01 00:00:00 UTC 2007

Vascular endothelial growth factor-D activates VEGFR-3 expressed in osteoblasts inducing their differentiation.

The Journal of biological chemistry

Orlandini M,Spreafico A,Bardelli M,Rocchigiani M,Salameh A,Nucciotti S,Capperucci C,Frediani B,Oliviero S

14-5988 was used in Immunofluorescence in immunohistochemistry on frozen tissues to show that in the long bones of newborn mice, vascular endothelial growth factor (VEGF)-D and VEGFR-3 are expressed in the osteoclasts of the growing plate.

Fri Jun 30 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: chylous ascites; FLT-4; Fms-like tyrosine kinase 4; receptor protein tyrosine kinase; soluble VEGFR 3; sVEGFR 3; Tyrosine-protein kinase receptor FLT4; Vascular endothelial growth factor receptor 3; vascular endothelial growth factor receptor-3; VEGF R3; VEGFR-3

Gene Aliases: AI323512; Chy; Flt-4; Flt4; VEGFR-3; VEGFR3

UniProt ID: (Mouse) P35917

Entrez Gene ID: (Mouse) 14257

Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity vascular endothelial growth factor-activated receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups growth factor binding protein phosphatase binding transmembrane signal receptor

Process(es)

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VEGF Receptor 3 Monoclonal Antibody (AFL4), eBioscience™

VEGF Receptor 3 Antibody (14-5988-85) in IHC

Product Details

14-5988-85

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions