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            • Primary Antibodies ›
            • CD8 Antibodies
            The product (cat # 16-0081-81) that you're looking for has been discontinued and replaced with 16-0081-82. The product (cat # 16-0081-81) that you're looking for has been discontinued. Please contact Technical Support with any questions.

            Invitrogen

            CD8a Monoclonal Antibody (53-6.7), Functional Grade, eBioscience™

            98 Published Figures
            222 References
            View all (611) CD8 antibodies
            Datasheet
            Protocols
            Datasheet
            Protocols

            Cite CD8a Monoclonal Antibody (53-6.7), Functional Grade, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (98)
            Application
            CD8a Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 99

            CD8a Antibody (16-0081-81) in Flow

            Mouse splenocytes were stained with 0.125 µg of Rat IgG2a kappa Isotype Control (Product # 14-4321-85) (blue histogram) or 0.125 µg of CD8a Monoclonal Antibody, Functional Grade (purple histogram) followed by IgG2a Monoclonal Antibody, PE (Product # 12-4817-82). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
            View Product

            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Immunocytochemistry (ICC/IF)
            CD8a Antibody in Immunocytochemistry (ICC/IF)
            CD8a Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
            CD8a Antibody in Immunocytochemistry (ICC/IF)
            CD8a Antibody in Immunocytochemistry (ICC/IF)
            CD8a Antibody in Immunocytochemistry (ICC/IF)
            CD8a Antibody in Immunocytochemistry (ICC/IF)
            CD8a Antibody in Immunocytochemistry (ICC/IF)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Western Blot (WB)
            CD8a Antibody in Western Blot, Flow Cytometry (WB, Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)

            Product Details

            16-0081-81

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            -
            View 2 publications 2 publications

            Immunohistochemistry (IHC)

            -
            View 24 publications 24 publications

            Immunohistochemistry (Frozen) (IHC (F))

            -
            View 1 publication 1 publication

            Immunocytochemistry (ICC/IF)

            -
            View 7 publications 7 publications

            Flow Cytometry (Flow)

            0.25 µg/test
            View 181 publications 181 publications

            ELISA (ELISA)

            -
            View 2 publications 2 publications

            Immunoprecipitation (IP)

            -
            View 1 publication 1 publication

            Neutralization (Neu)

            Assay-Dependent
            -

            Functional Assay (Functional)

            Assay-Dependent
            View 4 publications 4 publications
            Product Specifications

            Published species

            Hamster, Human, Mouse

            Host/Isotype

            Rat / IgG2a, kappa

            Recommended Isotype Control

            Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            53-6.7

            Conjugate

            Functional Grade
            • Unconjugated
            • Alexa Fluor 488 
            • Alexa Fluor 532 
            • Alexa Fluor 561
            • Alexa Fluor 660 
            • Alexa Fluor 700 
            • APC 
            • APC-eFluor 780
            • Biotin 
            • Brilliant UV 395
            • Brilliant UV 496
            • Brilliant UV 563
            • Brilliant UV 615
            • Brilliant UV 661
            • Brilliant UV 737
            • Brilliant UV 805
            • Brilliant Violet 421
            • Brilliant Violet 480
            • Brilliant Violet 605
            • Brilliant Violet 650
            • Brilliant Violet 711
            • Brilliant Violet 786
            • eFluor 450
            • eFluor 506
            • eFluor 615
            • eFluor 660
            • FITC
            • NovaFluor Blue 510
            • NovaFluor Blue 585
            • NovaFluor Blue 610-70S
            • NovaFluor Blue 660-120S
            • NovaFluor Blue 725
            • NovaFluor Blue 760
            • NovaFluor Blue 800
            • NovaFluor Red 710
            • NovaFluor UV 765
            • NovaFluor Violet 690
            • NovaFluor Violet 745
            • NovaFluor Violet 800
            • NovaFluor Yellow 590
            • NovaFluor Yellow 610
            • NovaFluor Yellow 660
            • NovaFluor Yellow 690
            • NovaFluor Yellow 700
            • NovaFluor Yellow 730
            • NovaFluor Yellow 810
            • PE 
            • PE-Cyanine5
            • PE-Cyanine5.5
            • PE-Cyanine7
            • PE-eFluor 610
            • PerCP-Cyanine5.5
            • PerCP-eFluor 710
            • Super Bright 436
            • Super Bright 600
            • Super Bright 645
            • Super Bright 702
            • Super Bright 780
            • Request custom conjugation

            Form

            Liquid

            Concentration

            1 mg/mL

            Storage conditions

            4°C

            Shipping conditions

            Wet ice

            RRID

            AB_468872

            Product Specific Information

            Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

            Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis. It has also been reported in T cell activation, isolation and depletion of CD8+ cells, and blocking of ligand binding.

            Applications Tested: The 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Storage and handling: Use in a sterile environment.

            Filtration: 0.2 µm post-manufacturing filtered.

            Purity: Greater than 90%, as determined by SDS-PAGE.

            Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

            Aggregation: Less than 10%, as determined by HPLC.

            Target Information

            Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            It has to be done as per old AB suggested Products section.
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            Performance Guarantee

            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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