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Invitrogen
CD16 Monoclonal Antibody (B73.1), Functional Grade, eBioscience™
Application
FIGURE: 1 / 2
CD16 Antibody (16-0167-85) in WB
European journal of immunology 2009 -
5 PI3K and GSK-3beta function to inhibit TNF-alpha release. (A) Cultured human primary monocytes were pretreated with PI3K kinase inhibitor LY294002 or GSK-3beta kinase inhibitor LiCl 1 h prior to the addition of anti-CD16 antibody (clone 3G8) or vehicle. Monocytes were collected 0, 15 or 60 min after the addition of the CD16 antibody or vehicle, and 10 mug of total protein was loaded in each lane for Weste... View More
Product Details
16-0167-85
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
B73.1
Conjugate
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11040983
Product Specific Information
Description: This B73.1 monoclonal antibody reacts with human CD16, a member of the Fc receptor family also known as Fc gamma RIIIa and Fc gamma RIIIb. This 50-65 kDa receptor is expressed on natural killer cells, neutrophils, monocytes, and macrophages. Crosslinking of CD16 in natural killer cells and monocytes results in activation of the MAPK and PI3K signaling pathways, thus regulating the production of TNF alpha, IL-6, and IL-1 beta.
This monoclonal antibody has been shown to block Fc receptor functions.
Applications Reported: This B73.1 antibody has been reported for use in functional blocking assays and flow cytometric analysis.
Applications Tested: This B73.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD16-I; CD16-II; CD16a; CD16a antigen; CD16b; CD32; CD32 receptor 2; CDw32; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III-A; Fc gamma receptor IIIa; Fc gamma receptor IIIb; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; Fc-gamma RIIIa; fc-gamma RIIIb; Fc-gamma-RIIb; Fc-gamma-RIIc; FCG2; FcgammaRIIIA; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; IgG Fc receptor III-1; IgG Fc receptor III-2; IgG Fc receptor III-A; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; neutrophil-specific antigen NA; RP11-5K23.1
Gene Aliases: CD16; CD16A; CD16B; FCG3; FCGR3; FCGR3A; FCGR3B; FCGRIII; FCR-10; FCRIII; FCRIIIA; FCRIIIb; IGFR3; IMD20
UniProt ID: (Human) P08637, (Human) O75015
Entrez Gene ID: (Human) 2214, (Human) 2215
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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