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            • Primary Antibodies ›
            • CSF1R (CD115) Antibodies

            Invitrogen

            CD115 (c-fms) Monoclonal Antibody (AFS98), APC, eBioscience™

            28 Published Figures
            90 References
            View all (82) CSF1R (CD115) antibodies
            Datasheet
            Protocols
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            Catalog # 17-1152-80
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            Cite CD115 (c-fms) Monoclonal Antibody (AFS98), APC, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (28)
            Application
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 29

            CD115 (c-fms) Antibody (17-1152-80) in Flow

            Staining of thioglycolate-induced peritoneal exudate cells (PECs) with 0.03 µg of Rat IgG2a kappa Isotype Control APC (Product # 17-4321-81) (blue histogram) or 0.03 µg of Anti-Mouse CD115 (c-fms) APC (purple histogram). Total viable cells were used for analysis.
            View Product

            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)
            CD115 (c-fms) Antibody in Flow Cytometry (Flow)

            Product Details

            17-1152-80

            Applications
            Tested Dilution
            Publications

            Flow Cytometry (Flow)

            0.06 µg/test
            View 90 publications 90 publications
            Product Specifications

            Species Reactivity

            Mouse

            Published species

            Fish, Mouse

            Host/Isotype

            Rat / IgG2a, kappa

            Recommended Isotype Control

            Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            AFS98

            Conjugate

            APC
            • Unconjugated
            • Alexa Fluor 488 
            • APC-eFluor 780
            • Biotin 
            • Brilliant UV 563
            • Functional Grade
            • NovaFluor Red 685
            • NovaFluor Red 710
            • NovaFluor Yellow 610
            • NovaFluor Yellow 730
            • PE 
            • PE-Cyanine7
            • PE-eFluor 610
            • PerCP-eFluor 710
            • Super Bright 436
            • Super Bright 600
            • Request custom conjugation

            Excitation/Emission Max

            651/660 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.2 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_1210790

            Product Specific Information

            Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

            Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis.

            Applications Tested: This AFS98 antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

            Filtration: 0.2 µm post-manufacturing filtered.

            Target Information

            CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            Bioinformatics

            Protein Aliases: c-fms; CD115; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; kinase CSFR; Macrophage colony-stimulating factor 1 receptor; OTTHUMP00000224093; Proto-oncogene c-Fms; proto-oncogene fms

            View more View less

            Gene Aliases: AI323359; CD115; CSF-1R; Csf1r; Csfmr; Fim-2; Fms; M-CSF-R; M-CSFR

            View more View less

            UniProt ID: (Mouse) P09581

            View more View less

            Entrez Gene ID: (Mouse) 12978

            View more View less

            Function(s)

            nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity macrophage colony-stimulating factor receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein phosphatase binding cytokine binding protein homodimerization activity transmembrane signal receptor

            Process(es)

            positive regulation of protein phosphorylation immune system process protein phosphorylation inflammatory response transmembrane receptor protein tyrosine kinase signaling pathway cell proliferation positive regulation of cell proliferation regulation of cell shape phosphorylation peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway hemopoiesis osteoclast differentiation positive regulation of cell migration ruffle organization cellular response to macrophage colony-stimulating factor stimulus macrophage colony-stimulating factor signaling pathway positive regulation of tyrosine phosphorylation of Stat3 protein innate immune response regulation of bone resorption cell-cell junction maintenance positive regulation of osteoclast differentiation phosphatidylinositol metabolic process protein autophosphorylation phosphatidylinositol-mediated signaling positive regulation of protein tyrosine kinase activity positive regulation of ERK1 and ERK2 cascade cellular response to cytokine stimulus positive regulation of protein serine/threonine kinase activity positive regulation of chemokine secretion positive regulation of cell motility regulation of actin cytoskeleton reorganization
            It has to be done as per old AB suggested Products section.
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