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Invitrogen

CD23 Monoclonal Antibody (B3B4), PE-Cyanine7, eBioscience™

10 Published Figures

47 References

View all (153) CD23 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (10)

Application

FIGURE: 1 / 11

CD23 Antibody (25-0232-81) in Flow

Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.06 µg of Anti-Mouse CD23 PE-Cyanine7 (right). Total viable cells were used for analysis.

Product Image Gallery

Product Details

25-0232-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 47 publications 47 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B3B4

Conjugate

PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_469603

Product Specific Information

Description: The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on resting conventional B cells, and its expression is modulated upon B-cell activation. B-1 cell lineage (CD5+ B cells) does not express CD23. Soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B-cell activation. CD21 is thought to bind to CD23.

Applications Reported: This B3B4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This B3B4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. Functionally, CD23 is involved in B cell growth and differentiation, and IgE production. Further, CD23 has a soluble form that is a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD23 monoclonal antibody (Product # 25-0232-82) in Flow Cytometry

Mon Feb 01 00:00:00 UTC 2021

Assessing Lupus-Like Disease in Murine Model Systems.

Current protocols

Lee HY,Celhar T,Fairhurst AM

25-0232-82 was used in Flow Cytometry to evaluate the underlying immunological features of the disease, as a more sensitive strategy to understand the disease itself and the mechanisms of potential novel therapeutics.

Mon Nov 01 00:00:00 UTC 2021

Neonatal Exposure to Commensal-Bacteria-Derived Antigens Directs Polysaccharide-Specific B-1 B Cell Repertoire Development.

Immunity

New JS,Dizon BLP,Fucile CF,Rosenberg AF,Kearney JF,King RG

25-0232-82 was used in Flow Cytometry to show that exposure to microbial antigens in early life determines the clonality of the mature B-1 B cell repertoire and ensuing antibody responses, with implications for vaccination approaches and schedules.

Tue Jul 14 00:00:00 UTC 2020

Isotype Switching Converts Anti-CD40 Antagonism to Agonism to Elicit Potent Antitumor Activity.

Cancer cell

Yu X,Chan HTC,Fisher H,Penfold CA,Kim J,Inzhelevskaya T,Mockridge CI,French RR,Duriez PJ,Douglas LR,English V,Verbeek JS,White AL,Tews I,Glennie MJ,Cragg MS

Published figure using CD23 monoclonal antibody (Product # 25-0232-82) in Flow Cytometry

Mon Jun 08 00:00:00 UTC 2020

Transplantation tolerance modifies donor-specific B cell fate to suppress de novo alloreactive B cells.

The Journal of clinical investigation

Khiew SH,Jain D,Chen J,Yang J,Yin D,Young JS,Dent A,Sciammas R,Alegre ML,Chong AS

25-0232 was used in Flow cytometry/Cell sorting to showed that alloreactive B cells were not deleted but rapidly lost their ability to differentiate into germinal center B cells and secrete donor-specific antibodies., by using a murine model of heart tolerance.

Wed Jul 01 00:00:00 UTC 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

25-0232 was used in Flow cytometry/Cell sorting to uncover a mechanism that controls myeloid regeneration and early lineage decisions in HSCs and could be targeted in LSCs to normalize leukemic myeloid cell production.

Mon Mar 02 00:00:00 UTC 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Mon Mar 02 00:00:00 UTC 2020

Activated PI3Kδ breaches multiple B cell tolerance checkpoints and causes autoantibody production.

The Journal of experimental medicine

Lau A,Avery DT,Jackson K,Lenthall H,Volpi S,Brigden H,Russell AJ,Bier J,Reed JH,Smart JM,Cole T,Choo S,Gray PE,Berglund LJ,Hsu P,Wong M,O'Sullivan M,Boztug K,Meyts I,Uzel G,Notarangelo LD,Brink R,Goodnow CC,Tangye SG,Deenick EK

Published figure using CD23 monoclonal antibody (Product # 25-0232-82) in Flow Cytometry

Mon Feb 03 00:00:00 UTC 2020

Activated PI3Kδ breaches multiple B cell tolerance checkpoints and causes autoantibody production.

The Journal of experimental medicine

Published figure using CD23 monoclonal antibody (Product # 25-0232-82) in Flow Cytometry

Mon Feb 03 00:00:00 UTC 2020

Site-specific ubiquitination of the E3 ligase HOIP regulates apoptosis and immune signaling.

The EMBO journal

Fennell LM,Gomez Diaz C,Deszcz L,Kavirayani A,Hoffmann D,Yanagitani K,Schleiffer A,Mechtler K,Hagelkruys A,Penninger J,Ikeda F

25-0232-82 was used in Flow Cytometry to propose that site-specific ubiquitination of HOIP regulates a LUBAC-dependent switch between survival and apoptosis in TNF signaling.

Tue Dec 15 00:00:00 UTC 2020

CD83 expression regulates antibody production in response to influenza A virus infection.

Virology journal

Akauliya M,Gautam A,Maharjan S,Park BK,Kim J,Kwon HJ

Published figure using CD23 monoclonal antibody (Product # 25-0232-82) in Flow Cytometry

Thu Dec 10 00:00:00 UTC 2020

miR-29 Sustains B Cell Survival and Controls Terminal Differentiation via Regulation of PI3K Signaling.

Cell reports

Hines MJ,Coffre M,Mudianto T,Panduro M,Wigton EJ,Tegla C,Osorio-Vasquez V,Kageyama R,Benhamou D,Perez O,Bajwa S,McManus MT,Ansel KM,Melamed D,Koralov SB

25-0232 was used in Flow cytometry/Cell sorting to suggest a critical role for the miR-29-PTEN-PI3K regulatory axis in mature B lymphocytes.

Tue Dec 01 00:00:00 UTC 2020

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production.

Journal of visualized experiments : JoVE

Upadhye A,Marshall M,Garmey JC,Bender TP,McNamara C

25-0232 was used in Flow cytometry/Cell sorting to describe a method to target B-1a cell migration to the bone marrow by retroviral-mediated overexpression of the C-X-C motif chemokine receptor 4 (CXCR4).

Sun May 31 00:00:00 UTC 2020

Estrogen Receptor Alpha Signaling Is Responsible for the Female Sex Bias in the Loss of Tolerance and Immune Cell Activation Induced by the Lupus Susceptibility Locus Sle1b.

Frontiers in immunology

Graham JH,Yoachim SD,Gould KA

25-0232 was used in Flow cytometry/Cell sorting to investigate the modulatory role of ERa signalling in Sle1 effects' female sex bias in mice.

Tue Jun 22 00:00:00 UTC 2021

Estrogen Receptor Alpha Signaling Is Responsible for the Female Sex Bias in the Loss of Tolerance and Immune Cell Activation Induced by the Lupus Susceptibility Locus Sle1b.

Frontiers in immunology

Graham JH,Yoachim SD,Gould KA

25-0232 was used in Flow cytometry/Cell sorting to investigate the modulatory role of ERa signalling in Sle1 effects' female sex bias in mice.

Tue Jun 22 00:00:00 UTC 2021

Peritoneal Cells Mediate Immune Responses and Cross-Protection Against Influenza A Virus.

Frontiers in immunology

Gautam A,Park BK,Kim TH,Akauliya M,Kim D,Maharjan S,Park J,Kim J,Lee H,Park MS,Lee Y,Kwon HJ

Published figure using CD23 monoclonal antibody (Product # 25-0232-82) in Flow Cytometry

Mon Aug 17 00:00:00 UTC 2020

Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome.

Nature communications

Komban RJ,Strömberg A,Biram A,Cervin J,Lebrero-Fernández C,Mabbott N,Yrlid U,Shulman Z,Bemark M,Lycke N

Published figure using CD23 monoclonal antibody (Product # 25-0232-82) in Flow Cytometry

Mon Jun 03 00:00:00 UTC 2019

MALT1 Proteolytic Activity Suppresses Autoimmunity in a T Cell Intrinsic Manner.

Frontiers in immunology

Demeyer A,Skordos I,Driege Y,Kreike M,Hochepied T,Baens M,Staal J,Beyaert R

25-0232 was used in Flow cytometry/Cell sorting to establish that suppression of autoimmune responses is due to the T-cell intrinsic role of MALT1 proteolytic activity.

Mon Oct 05 00:00:00 UTC 2020

c-Myb Exacerbates Atherosclerosis through Regulation of Protective IgM-Producing Antibody-Secreting Cells.

Cell reports

Shikatani EA,Besla R,Ensan S,Upadhye A,Khyzha N,Li A,Emoto T,Chiu F,Degousee N,Moreau JM,Perry HM,Thayaparan D,Cheng HS,Pacheco S,Smyth D,Noyan H,Zavitz CCJ,Bauer CMT,Hilgendorf I,Libby P,Swirski FK,Gommerman JL,Fish JE,Stampfli MR,Cybulsky MI,Rubin BB,Paige CJ,Bender TP,McNamara CA,Husain M,Robbins CS

25-0232-82 was used in Flow Cytometry to identify the nuclear transcription factor c-Myb as an important mediator of atherosclerotic disease in mice.

Tue May 21 00:00:00 UTC 2019

TLR9 Signaling Suppresses the Canonical Plasma Cell Differentiation Program in Follicular B Cells.

Frontiers in immunology

Baptista BJA,Granato A,Canto FB,Montalvão F,Tostes L,de Matos Guedes HL,Coutinho A,Bellio M,Vale AM,Nobrega A

25-0232 was used in Flow cytometry/Cell sorting to identify unexpected, opposing roles for Toll-like receptor4 and TLR9 in the control of plasma B cell differentiation programmes.

Mon Sep 30 00:00:00 UTC 2019

IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate.

eLife

Noviski M,Mueller JL,Satterthwaite A,Garrett-Sinha LA,Brombacher F,Zikherman J

25-0232-82 was used in Flow Cytometry to study how IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate.

Fri Mar 09 00:00:00 UTC 2018

Prolonged Deleterious Influences of Chemotherapeutic Agent CPT-11 on Resident Peritoneal Macrophages and B1 Cells.

Frontiers in immunology

Bai WJ,Li CG,Zhang CC,Xu LH,Zeng QZ,Hu B,Hong Z,He XH,Ouyang DY

Published figure using CD23 monoclonal antibody (Product # 25-0232-82) in Flow Cytometry

Wed Nov 20 00:00:00 UTC 2019

B cell subsets are modulated during allergic airway inflammation but are not required for the development of respiratory tolerance in a murine model.

European journal of immunology

Habener A,Behrendt AK,Skuljec J,Jirmo AC,Meyer-Bahlburg A,Hansen G

25-0232 was used in Flow cytometry/Cell sorting to investigate how B cells are affected by induction of allergic airway inflammation and respiratory tolerance.

Wed Mar 01 00:00:00 UTC 2017

Loss of IP₃ Receptor-Mediated Ca²⁺ Release in Mouse B Cells Results in Abnormal B Cell Development and Function.

Journal of immunology (Baltimore, Md. : 1950)

Tang H,Wang H,Lin Q,Fan F,Zhang F,Peng X,Fang X,Liu J,Ouyang K

25-0232 was used in Flow cytometry/Cell sorting to determine the function of 1,4,5-trisphosphate receptor-mediated Ca2+ release in B cells.

Sat Jul 15 00:00:00 UTC 2017

Age-Associated B Cells Express a Diverse Repertoire of V_H and Vκ Genes with Somatic Hypermutation.

Journal of immunology (Baltimore, Md. : 1950)

Russell Knode LM,Naradikian MS,Myles A,Scholz JL,Hao Y,Liu D,Ford ML,Tobias JW,Cancro MP,Gearhart PJ

25-0232 was used in Flow cytometry/Cell sorting to show that quiescent ABCs are Ag-experienced cells that accumulate during T cell-dependent responses to diverse Ags during the life of an individual.

Wed Mar 01 00:00:00 UTC 2017

The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.

Cell

Damgaard RB,Walker JA,Marco-Casanova P,Morgan NV,Titheradge HL,Elliott PR,McHale D,Maher ER,McKenzie ANJ,Komander D

25-0232 was used in Flow cytometry/Cell sorting to study how OTULIN may be critical in restraining life-threatening spontaneous inflammation and maintaining immune homeostasis.

Thu Aug 25 00:00:00 UTC 2016

Galectin-1 drives lymphoma CD20 immunotherapy resistance: validation of a preclinical system to identify resistance mechanisms.

Blood

Lykken JM,Horikawa M,Minard-Colin V,Kamata M,Miyagaki T,Poe JC,Tedder TF

25-0232 was used in Flow cytometry/Cell sorting to elucidate the mechanisms through which non-Hodgkin lymphomas initially resist or gain resistance to immunotherapy.

Thu Apr 14 00:00:00 UTC 2016

Prolactin Rescues Immature B-Cells from Apoptosis Induced by B-Cell Receptor Cross-Linking.

Journal of immunology research

Flores-Fernández R,Blanco-Favela F,Fuentes-Pananá EM,Chávez-Sánchez L,Gorocica-Rosete P,Pizaña-Venegas A,Chávez-Rueda AK

25-0232 was used in Flow cytometry/Cell sorting to determine the effect of prolactin in an in vitro system of B-cell tolerance, showing that it rescues immature B-cells from apoptosis induced by B-cell receptor cross-linking.

Mon Mar 06 00:00:00 UTC 2017

IgD attenuates the IgM-induced anergy response in transitional and mature B cells.

Nature communications

Sabouri Z,Perotti S,Spierings E,Humburg P,Yabas M,Bergmann H,Horikawa K,Roots C,Lambe S,Young C,Andrews TD,Field M,Enders A,Reed JH,Goodnow CC

25-0232 was used in Flow cytometry/Cell sorting to define the RNA landscape of the in vivo anergy response, comprising 220 induced sequences including a core set of 97.

Thu Nov 10 00:00:00 UTC 2016

ATP11C Facilitates Phospholipid Translocation across the Plasma Membrane of All Leukocytes.

PloS one

Yabas M,Jing W,Shafik S,Bröer S,Enders A

25-0232 was used in Flow cytometry/Cell sorting to identify the P4-type ATPase ATP11C as a flippase that selectively transports phosphatidylserine and phosphatidylethanolamine to the inner leaflet of the plasma membrane in immune cells.

Mon Jul 25 00:00:00 UTC 2016

Mantle cell lymphoma in cyclin D1 transgenic mice with Bim-deficient B cells.

Blood

Katz SG,Labelle JL,Meng H,Valeriano RP,Fisher JK,Sun H,Rodig SJ,Kleinstein SH,Walensky LD

Published figure using CD23 monoclonal antibody (Product # 25-0232-82) in Flow Cytometry

Thu Feb 06 00:00:00 UTC 2014

IL-4 regulates Bim expression and promotes B cell maturation in synergy with BAFF conferring resistance to cell death at negative selection checkpoints.

Journal of immunology (Baltimore, Md. : 1950)

Granato A,Hayashi EA,Baptista BJ,Bellio M,Nobrega A

25-0232 was used in Flow cytometry/Cell sorting to analyze the effect of IL-4 on B cell maturation, and its influence on BCR-mediated negative selection.

Sun Jun 15 00:00:00 UTC 2014

Enhanced auto-antibody production and Mott cell formation in FcμR-deficient autoimmune mice.

International immunology

Honjo K,Kubagawa Y,Suzuki Y,Takagi M,Ohno H,Bucy RP,Izui S,Kubagawa H

25-0232 was used in Flow cytometry/Cell sorting to determine the impact of Fcmr ablation on autoimmunity.

Mon Dec 01 00:00:00 UTC 2014

Intrinsic molecular factors cause aberrant expansion of the splenic marginal zone B cell population in nonobese diabetic mice.

Journal of immunology (Baltimore, Md. : 1950)

Stolp J,Mariño E,Batten M,Sierro F,Cox SL,Grey ST,Silveira PA

25-0232 was used in Flow cytometry/Cell sorting to investigate the underlying causes of augmented marginal zone B cell production in NOD mice.

Mon Jul 01 00:00:00 UTC 2013

Galectin 1 modulates plasma cell homeostasis and regulates the humoral immune response.

Journal of immunology (Baltimore, Md. : 1950)

Anginot A,Espeli M,Chasson L,Mancini SJ,Schiff C

25-0232 was used in Flow cytometry/Cell sorting to elucidate the pattern of GAL1 expression and the function of GAL1 in B cells.

Sat Jun 01 00:00:00 UTC 2013

Prolactin levels correlate with abnormal B cell maturation in MRL and MRL/lpr mouse models of systemic lupus erythematosus-like disease.

Clinical & developmental immunology

Legorreta-Haquet MV,Flores-Fernández R,Blanco-Favela F,Fuentes-Pananá EM,Chávez-Sánchez L,Hernández-González R,Tesoro-Cruz E,Arriaga-Pizano L,Chávez-Rueda AK

25-0232 was used in Flow cytometry/Cell sorting to determine the level of PRL receptor expression during bone-marrow B-cell development and assess relevance in disease outcomes in lupus-prone murine models.

Mon Aug 04 00:00:00 UTC 2014

IKKα-mediated signaling circuitry regulates early B lymphopoiesis during hematopoiesis.

Blood

Balkhi MY,Willette-Brown J,Zhu F,Chen Z,Liu S,Guttridge DC,Karin M,Hu Y

25-0232-82 was used in Flow Cytometry to demonstrate that IKKα regulates B-lineage commitment via combined canonical and noncanonical NF-κB transcriptional activities to target Pax5 expression during hematopoiesis.

Thu Jun 07 00:00:00 UTC 2012

Splenic morphological changes are accompanied by altered baseline immunity in a mouse model of sickle-cell disease.

The American journal of pathology

Szczepanek SM,McNamara JT,Secor ER,Natarajan P,Guernsey LA,Miller LA,Ballesteros E,Jellison E,Thrall RS,Andemariam B

25-0232 was used in Flow cytometry/Cell sorting to investigate the relationship between functional asplenia from infarctions and increased infectious mortality in a mouse model of sickle-cell disease.

Thu Nov 01 00:00:00 UTC 2012

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using CD23 monoclonal antibody (Product # 25-0232-82) in Flow Cytometry

Sat Dec 01 00:00:00 UTC 2012

The role of sphingosine-1-phosphate transporter Spns2 in immune system function.

Journal of immunology (Baltimore, Md. : 1950)

Nijnik A,Clare S,Hale C,Chen J,Raisen C,Mottram L,Lucas M,Estabel J,Ryder E,Adissu H,Adams NC,Ramirez-Solis R,White JK,Steel KP,Dougan G,Hancock RE

25-0232 was used in Flow cytometry/Cell sorting to study the roles of Spns2 in mammals and its involvement in the different S1P-dependent physiological processes.

Sun Jul 01 00:00:00 UTC 2012

Marginal zone B cells regulate antigen-specific T cell responses during infection.

Journal of immunology (Baltimore, Md. : 1950)

Bankoti R,Gupta K,Levchenko A,Stäger S

25-0232 was used in Flow cytometry/Cell sorting to investigate the role of B cells in the regulation of antigen-specific CD8+ T cell responses in a murine model of visceral leishmaniasis.

Sun Apr 15 00:00:00 UTC 2012

Direct reduction of antigen receptor expression in polyclonal B cell populations developing in vivo results in light chain receptor editing.

Journal of immunology (Baltimore, Md. : 1950)

Shen S,Manser T

25-0232 was used in Flow cytometry/Cell sorting to test the role of BCR expression plays in receptor editing in an polyclonal, in vivo population of B cells.

Sun Jan 01 00:00:00 UTC 2012

The specialized unfolded protein response of B lymphocytes: ATF6α-independent development of antibody-secreting B cells.

Molecular immunology

Aragon IV,Barrington RA,Jackowski S,Mori K,Brewer JW

25-0232 was used in Flow cytometry/Cell sorting to demonstrate that a specialised B cell UPR relies exclusively on the IRE1-XBP1 pathway to remodel the ER and expand secretory capacity.

Sun Jul 01 00:00:00 UTC 2012

Increased levels of prolactin receptor expression correlate with the early onset of lupus symptoms and increased numbers of transitional-1 B cells after prolactin treatment.

BMC immunology

Ledesma-Soto Y,Blanco-Favela F,Fuentes-Pananá EM,Tesoro-Cruz E,Hernández-González R,Arriaga-Pizano L,Legorreta-Haquet MV,Montoya-Diaz E,Chávez-Sánchez L,Castro-Mussot ME,Chávez-Rueda AK

25-0232 was used in Flow cytometry/Cell sorting to determine if different splenic B cell subsets express the prolactin receptor and if the presence of prolactin influences these B cell subsets and correlates with development of lupus.

Fri Mar 09 00:00:00 UTC 2012

Cellular competition independent of BAFF/B lymphocyte stimulator results in low frequency of an autoreactive clonotype in mature polyclonal B cell compartments.

Journal of immunology (Baltimore, Md. : 1950)

Nikbakht N,Migone TS,Ward CP,Manser T

25-0232 was used in Flow cytometry/Cell sorting to investigate the role of the peripheral B cell pro-survival cytokine BAFF/BLyS in the regulation of immunological tolerance and autoreactive cells.

Fri Jul 01 00:00:00 UTC 2011

Impaired B cell development in the absence of Krüppel-like factor 3.

Journal of immunology (Baltimore, Md. : 1950)

Vu TT,Gatto D,Turner V,Funnell AP,Mak KS,Norton LJ,Kaplan W,Cowley MJ,Agenès F,Kirberg J,Brink R,Pearson RC,Crossley M

25-0232 was used in Flow cytometry/Cell sorting to examine the function of Klf3 in B cell development by studying B lymphopoiesis in a Klf3 knockout mouse model.

Tue Nov 15 00:00:00 UTC 2011

HVCN1 modulates BCR signal strength via regulation of BCR-dependent generation of reactive oxygen species.

Nature immunology

Capasso M,Bhamrah MK,Henley T,Boyd RS,Langlais C,Cain K,Dinsdale D,Pulford K,Khan M,Musset B,Cherny VV,Morgan D,Gascoyne RD,Vigorito E,DeCoursey TE,MacLennan IC,Dyer MJ

25-0232 was used in Flow cytometry/Cell sorting to investigate how HVCN1 modulates BCR signal strength through regulation of BCR-dependent generation of reactive oxygen species.

Mon Mar 01 00:00:00 UTC 2010

Conditional TPM3-ALK and NPM-ALK transgenic mice develop reversible ALK-positive early B-cell lymphoma/leukemia.

Blood

Giuriato S,Foisseau M,Dejean E,Felsher DW,Al Saati T,Demur C,Ragab A,Kruczynski A,Schiff C,Delsol G,Meggetto F

25-0232 was used in Flow cytometry/Cell sorting to develop conditional mouse models for anaplastic lymphoma kinase-induced lymphomagenesis.

Thu May 20 00:00:00 UTC 2010

Peripheral B cell tolerance and function in transgenic mice expressing an IgD superantigen.

Journal of immunology (Baltimore, Md. : 1950)

Duong BH,Ota T,Aït-Azzouzene D,Aoki-Ota M,Vela JL,Huber C,Walsh K,Gavin AL,Nemazee D

25-0232 was used in Flow cytometry/Cell sorting to generate mice which ubiquitously expressed a membrane-bound IgD-superantigen, to provide a model which should facilitate studies of peripheral B cell tolerance in future.

Thu Apr 15 00:00:00 UTC 2010

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using CD23 monoclonal antibody (Product # 25-0232-82) in Flow Cytometry

Thu May 07 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: CD23; FC epsilon RII; Fc-epsilon-RII; FceRII; Low affinity immunoglobulin epsilon Fc receptor; low-affinity IgE receptor; Lymphocyte IgE receptor

Gene Aliases: CD23; Fce2; Fcer2; Fcer2a; Ly-42

UniProt ID: (Mouse) P20693

Entrez Gene ID: (Mouse) 14128

Function(s)

Process(es)

positive regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of nitric-oxide synthase activity positive regulation of killing of cells of other organism positive regulation of nitric-oxide synthase biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD23 Monoclonal Antibody (B3B4), PE-Cyanine7, eBioscience™

CD23 Antibody (25-0232-81) in Flow

Product Details

25-0232-81

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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