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            • Primary Antibodies ›
            • CD23 Antibodies

            Invitrogen

            CD23 Monoclonal Antibody (B3B4), PE-Cyanine7, eBioscience™

            10 Published Figures
            47 References
            View all (153) CD23 antibodies
            Datasheet
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            Catalog # 25-0232-81
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            Cite CD23 Monoclonal Antibody (B3B4), PE-Cyanine7, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (10)
            Application
            CD23 Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 11

            CD23 Antibody (25-0232-81) in Flow

            Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.06 µg of Anti-Mouse CD23 PE-Cyanine7 (right). Total viable cells were used for analysis.
            View Product

            CD23 Antibody in Flow Cytometry (Flow)
            CD23 Antibody in Flow Cytometry (Flow)
            CD23 Antibody in Flow Cytometry (Flow)
            CD23 Antibody in Flow Cytometry (Flow)
            CD23 Antibody in Flow Cytometry (Flow)
            CD23 Antibody in Flow Cytometry (Flow)
            CD23 Antibody in Flow Cytometry (Flow)
            CD23 Antibody in Flow Cytometry (Flow)
            CD23 Antibody in Flow Cytometry (Flow)
            CD23 Antibody in Flow Cytometry (Flow)
            CD23 Antibody in Flow Cytometry (Flow)

            Product Details

            25-0232-81

            Applications
            Tested Dilution
            Publications

            Flow Cytometry (Flow)

            0.125 µg/test
            View 47 publications 47 publications
            Product Specifications

            Species Reactivity

            Mouse

            Published species

            Mouse

            Host/Isotype

            Rat / IgG2a, kappa

            Recommended Isotype Control

            Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            B3B4

            Conjugate

            PE-Cyanine7
            • Unconjugated
            • Biotin 
            • Brilliant UV 805
            • eFluor 450
            • eFluor 660
            • FITC
            • NovaFluor Blue 510
            • NovaFluor Blue 610-70S
            • NovaFluor Blue 660-120S
            • NovaFluor Red 685
            • NovaFluor Red 710
            • NovaFluor Red 725
            • NovaFluor Yellow 610
            • NovaFluor Yellow 660
            • NovaFluor Yellow 755
            • PE 
            • PerCP-eFluor 710
            • Super Bright 436
            • Super Bright 600
            • Super Bright 645
            • Request custom conjugation

            Excitation/Emission Max

            569/780 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.2 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_469603

            Product Specific Information

            Description: The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on resting conventional B cells, and its expression is modulated upon B-cell activation. B-1 cell lineage (CD5+ B cells) does not express CD23. Soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B-cell activation. CD21 is thought to bind to CD23.

            Applications Reported: This B3B4 antibody has been reported for use in flow cytometric analysis.

            Applications Tested: This B3B4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

            Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

            Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

            Filtration: 0.2 µm post-manufacturing filtered.

            Target Information

            CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. Functionally, CD23 is involved in B cell growth and differentiation, and IgE production. Further, CD23 has a soluble form that is a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            Bioinformatics

            Protein Aliases: CD23; FC epsilon RII; Fc-epsilon-RII; FceRII; Low affinity immunoglobulin epsilon Fc receptor; low-affinity IgE receptor; Lymphocyte IgE receptor

            View more View less

            Gene Aliases: CD23; Fce2; Fcer2; Fcer2a; Ly-42

            View more View less

            UniProt ID: (Mouse) P20693

            View more View less

            Entrez Gene ID: (Mouse) 14128

            View more View less

            Function(s)

            IgE binding carbohydrate binding metal ion binding membrane traffic protein

            Process(es)

            positive regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of nitric-oxide synthase activity positive regulation of killing of cells of other organism positive regulation of nitric-oxide synthase biosynthetic process
            It has to be done as per old AB suggested Products section.
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            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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