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Application
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CD199 (CCR9) Antibody (25-1991-80) in Flow
Staining of BALB/c thymocytes with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82), Anti-Mouse CD8a APC (Product # 17-0081-82) and 0.25 µg of Mouse IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4724-81) (left) or 0.25 µg of Anti-Mouse CD199 (CCR9) PE-Cyanine7 (right). Samples were gated on CD4 single positive (orange histogram), CD8 single positive (blue histogram), or CD4+CD8+ double positive (purple histogram) cells.
Product Details
25-1991-80
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioCW-1.2 (CW-1.2)
Conjugate
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_10854724
Product Specific Information
Description: The eBioCW-1.2 monoclonal antibody reacts with mouse CCR9 (CD199), which is the receptor for thymus-expressed chemokine (TECK). CCR9 is a member of the G protein coupled receptor (GPCR) supergene family, and is involved in trafficking of T cell progenitors within the thymus. CCR9 expression during thymocyte development commences at the double-negative (DN) 3 stage (CD4-CD8-CD25+CD44-), peaks in the double-positive (DP) stage (CD4+CD8+CD25-CD44-), and is down-regulated in commited CD4+ or CD8+ single-positive (SP) thymoyctes. CCR9-deficient mice show a mild impairment in thymoycte development. In the periphery, CCR9 is thought to be expressed on naive CD8+ T cells, but not on naive CD4+ T cells.
Applications Reported: This eBioCW-1.2 (CW-1.2) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioCW-1.2 (CW-1.2) antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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