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Invitrogen
Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Application
FIGURE: 1 / 14
Rabbit IgG (H+L) Secondary Antibody (31466) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1) and K-562 (Lane 2). The blots were probed with Anti-CacyBP Rabbit Polyclonal Antibody (Product # 720326, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # 31460) at dilutions 1:5,000 (Fig. 1), 1:100,000 (Fig. 2) and 1:200,000 (Fig. 3). A 30 kDa ... View More
Product Details
31466
Applications
Tested Dilution
Western Blot (WB)
1:5,000-1:200,000
Immunohistochemistry (Paraffin) (IHC (P))
1:100-1:1,000
Immunocytochemistry (ICC/IF)
1:500-1:5,000
Immunoprecipitation (IP)
1:500-1:5,000
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Purified Rabbit IgG, whole molecule
Conjugate
HRP
Form
Lyophilized
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.6, with 15mg/mL BSA, 50mM sucrose, proprietary stabilizer
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10960844
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Product # 31466 has been successfully used in Western blot, IHC and IP applications.
Product # 31466 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species and with SuperBlock™ Blocking Buffers.
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Reconstitute with 0.2 mL of distilled water (1 mg/mL after restoration).
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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