Search Thermo Fisher Scientific
- Order Status
- Quick Order
-
Don't have an account ? Create Account
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Rabbit anti-Human IgG Fc Secondary Antibody, FITC
Application
FIGURE: 1 / 1
Human IgG Fc Secondary Antibody (31535) in Flow
CAR architecture, expression on engineered T cells, and binding of CAR-T cells to target cells. (A) The architecture of three different CAR constructs used in this study. In the original construct (tMUC1-CAR), scFv of TAB004 Ab is linked to CD28 transmembrane (TM) domain followed by CD28 and CD3ζ intracellular domains in a retroviral plasmid. CD8a leader sequence was used as signal peptide for cell membrane... View More
Product Details
31535
Applications
Tested Dilution
Immunohistochemistry (IHC)
1:50 - 1:200
Immunocytochemistry (ICC/IF)
1:50 - 1:200
Flow Cytometry (Flow)
1:50 - 1:200
Immunoprecipitation (IP)
1:50 - 1:200
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Lyophilized
Concentration
1.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.6, with 15mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_228408
Target
IgG Fc
Antibody Form
Whole Antibody
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
This antibody has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Antibody Specificity: This antibody reacts with the heavy chains of human IgG, but not with the light chains on most human immunoglobulins. No antibody was detected against human IgM, IgA, or non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Fluorophore: Fluorescein-5-isothiocyanate (FITC-isomer 1)
Amax = 492 nm; Emax = 520 nm
Fluorophore/Protein: 7.5 µg/mg; 2.8 moles FITC per mole IgG
Restoration and Storage: Store product at 4°C until opened. Restore with 1.1 mL distilled water (1.5 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Country of Origin: USA
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support