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            • Primary Antibodies ›
            • SPARC Antibodies

            Invitrogen

            SPARC Monoclonal Antibody (ON1-1)

            7 Published Figures
            33 References
            View all (54) SPARC antibodies
            Datasheet
            Protocols
            Questions & Answers
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            Catalog # 33-5500
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            Price (USD)
            508.00
            100 µg
             
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            Cite SPARC Monoclonal Antibody (ON1-1)

            • Published Figures (7)
            Application
            SPARC Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 7

            SPARC Antibody (33-5500) in ICC/IF

            Asian journal of andrology 2020 - Figure 2 Basal expression, silencing, and overexpression of SPARC in PCa cell lines. (a) Representative image of western blot against SPARC in PCa cell lines 22Rv1, LNCaP, DU145 and PC3. (b) RT-qPCR of SPARC in PCa cell lines. DeltaDeltaCt was obtained after normalizing to PUM1 and PC3 cell lines ( n = 3). (c and d) PC3 cells were stable transduced with shRNA against SPARC (ShSPARC) or scramble (ShScr). LNC... View More
            View Product

            SPARC Antibody in Immunocytochemistry (ICC/IF)
            SPARC Antibody in Immunohistochemistry (IHC)
            SPARC Antibody in Immunohistochemistry (IHC)
            SPARC Antibody in Immunohistochemistry (IHC)
            SPARC Antibody in Western Blot (WB)
            SPARC Antibody in Western Blot (WB)
            SPARC Antibody in ELISA (ELISA)

            Product Details

            33-5500

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            5-10 µg/mL
            View 4 publications 4 publications

            Immunohistochemistry (IHC)

            5-10 µg/mL
            View 19 publications 19 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            -
            View 4 publications 4 publications

            Immunocytochemistry (ICC/IF)

            -
            View 3 publications 3 publications

            Flow Cytometry (Flow)

            1 µg/mL
            -

            ELISA (ELISA)

            Assay-dependent
            View 2 publications 2 publications

            Miscellaneous PubMed (Misc)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Bovine, Human, Pig, Rabbit

            Published species

            Dog, Human, Rat

            Host/Isotype

            Mouse / IgG1

            Class

            Monoclonal

            Type

            Antibody

            Clone

            ON1-1

            Immunogen

            Full-length Bovine bone osteonectin (SPARC)
            View immunogen

            Conjugate

            Unconjugated

            Form

            Lyophilized

            Concentration

            2.0 mg/mL

            Purification

            Ion-exchange chromatography

            Storage buffer

            PBS, pH 7.4, with 1% BSA

            Contains

            no preservative

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2533126

            Product Specific Information

            Source: Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3-X63-Ag8-U1 with spleen cells of BALB/c mouse after immunization with bovine bone osteonectin (SPARC). The monoclonal antibody was harvested from ascitic fluid.

            Specificity: This antibody reacts with both bone derived and platelet derived Osteonectin (ELISA). This antibody does not react with non-activated platelet and reacts with thrombin-stimulated platelet (flow cytometric analysis). This antibody reacts with human and bovine Osteonectin (Western blot analysis). This antibody cross reacts with rabbit and porcine Osteonectin. (ELISA)

            Storage: 4°C. This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

            Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

            Target Information

            SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: Basement membrane protein 40; Basement-membrane protein 40; BM-40; BM40; Cysteine rich protein; ON; Osteonectin; osteonectin precursor; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic and rich in cysteine (SPARC); secreted protein, acidic, cysteine-rich (osteonectin); SPARC

            View more View less

            Gene Aliases: BM-40; OI17; ON; SPARC

            View more View less

            UniProt ID: (Bovine) P13213, (Human) P09486, (Pig) P20112

            View more View less

            Entrez Gene ID: (Bovine) 282077, (Human) 6678, (Pig) 595124, (Rabbit) 100009355

            View more View less

            Function(s)

            calcium ion binding protein binding collagen binding extracellular matrix binding extracellular matrix glycoprotein

            Process(es)

            ossification negative regulation of endothelial cell proliferation platelet degranulation receptor-mediated endocytosis signal transduction heart development response to gravity response to lead ion positive regulation of endothelial cell migration negative regulation of angiogenesis regulation of cell morphogenesis extracellular matrix organization lung development response to lipopolysaccharide response to L-ascorbic acid response to cytokine wound healing response to peptide hormone response to ethanol response to cadmium ion inner ear development response to glucocorticoid response to cAMP response to calcium ion bone development cellular response to growth factor stimulus
            It has to be done as per old AB suggested Products section.
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