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            • Primary Antibodies ›
            • CD3 Antibodies

            Invitrogen

            CD3 Monoclonal Antibody (17A2), eFluor™ 450, eBioscience™

            78 Published Figures
            166 References
            View all (540) CD3 antibodies
            Datasheet
            Protocols
            Questions & Answers
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            Catalog # 48-0032-82
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            Price (USD)
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            324.00
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            Cite CD3 Monoclonal Antibody (17A2), eFluor™ 450, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (78)
            Application
            CD3 Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 79

            CD3 Antibody (48-0032-82) in Flow

            Staining of C57Bl/6 splenocytes with FITC anti-mo/hu B220 (RA3-6B2) (Product # 11-0452-82) and 0.25 µg of eFluor® 450 Rat IgG2b Isotype Control (Product # 48-4031-82) (left) or 0.25 µg of eFluor® 450 anti-mouse CD3 (17A2) (right). Total viable cells were used for analysis.
            View Product

            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Immunohistochemistry (IHC)
            CD3 Antibody in Immunocytochemistry (ICC/IF)
            CD3 Antibody in Immunocytochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC (P))
            CD3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
            CD3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
            CD3 Antibody in Immunocytochemistry (ICC/IF)
            CD3 Antibody in Immunohistochemistry (Frozen) (IHC (F))
            CD3 Antibody in Immunohistochemistry (IHC)
            CD3 Antibody in Immunohistochemistry (IHC)
            CD3 Antibody in Immunohistochemistry (IHC)
            CD3 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
            CD3 Antibody in Immunohistochemistry (IHC)
            CD3 Antibody in Immunohistochemistry (IHC)
            CD3 Antibody in Immunohistochemistry (IHC)
            CD3 Antibody in Immunohistochemistry (IHC)
            CD3 Antibody in Immunohistochemistry (IHC)
            CD3 Antibody in Immunohistochemistry (IHC)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in Flow Cytometry (Flow)
            CD3 Antibody in T-Cell Activation (TCA)

            Product Details

            48-0032-82

            Applications
            Tested Dilution
            Publications

            Immunohistochemistry (IHC)

            -
            View 13 publications 13 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            -
            View 1 publication 1 publication

            Immunohistochemistry (Frozen) (IHC (F))

            -
            View 2 publications 2 publications

            Immunocytochemistry (ICC/IF)

            -
            View 5 publications 5 publications

            Flow Cytometry (Flow)

            0.5 µg/test
            View 144 publications 144 publications

            T-Cell Activation (TCA)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Mouse

            Published species

            Human, Mouse

            Host/Isotype

            Rat / IgG2b, kappa

            Recommended Isotype Control

            Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 450, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            17A2

            Conjugate

            eFluor™ 450
            • Unconjugated
            • Alexa Fluor 488 
            • Alexa Fluor 532 
            • Alexa Fluor 700 
            • APC 
            • APC-eFluor 780
            • Biotin 
            • Brilliant UV 395
            • Brilliant UV 496
            • Brilliant UV 737
            • Brilliant UV 805
            • Brilliant Violet 421
            • Brilliant Violet 480
            • Brilliant Violet 650
            • eFluor 506
            • eFluor 660
            • FITC
            • Functional Grade
            • PE 
            • PE-Cyanine5
            • PE-Cyanine7
            • PE-eFluor 610
            • PerCP-eFluor 710
            • Super Bright 600
            • Super Bright 702
            • Super Bright 780
            • Request custom conjugation

            Excitation/Emission Max

            405/445 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.2 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_1272193

            Product Specific Information

            Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.

            Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis.

            Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

            Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

            Filtration: 0.2 µm post-manufacturing filtered.

            Target Information

            The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            Bioinformatics

            Protein Aliases: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

            View more View less

            Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz

            View more View less

            UniProt ID: (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942

            View more View less

            Entrez Gene ID: (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502

            View more View less

            Function(s)

            transmembrane signaling receptor activity protein binding identical protein binding protein heterodimerization activity signal transducer activity SH3 domain binding defense/immunity protein immunoglobulin receptor superfamily

            Process(es)

            cell surface receptor signaling pathway T cell receptor signaling pathway protein complex assembly T cell activation positive thymic T cell selection positive regulation of T cell anergy response to nutrient T cell costimulation positive regulation of interferon-gamma production positive regulation of interleukin-4 production positive regulation of T cell proliferation negative thymic T cell selection positive regulation of interleukin-2 biosynthetic process negative regulation of smoothened signaling pathway positive regulation of alpha-beta T cell proliferation lymphocyte activation positive regulation of peptidyl-tyrosine phosphorylation positive regulation of calcium-mediated signaling positive regulation of T cell activation apoptotic signaling pathway establishment or maintenance of cell polarity protein transport regulation of lymphocyte apoptotic process
            It has to be done as per old AB suggested Products section.
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