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Invitrogen
Themis Monoclonal Antibody (1TMYS), eFluor™ 660, eBioscience™
Application
FIGURE: 1 / 1
Themis Antibody (50-5918-82) in Flow
LEFT: Surface staining of mouse splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) followed by intracellular staining with 0.125 µg of Anti-Mouse Themis eFluor® 660, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis; quadrant lines are based on isotype control. RIGHT: Mouse thymocytes were surface stained with Anti-Mouse CD4 FITC (Product # 11-0042-82), Anti-Mouse CD8a eFluor® 450 (Product # 48-0081-82), Anti-Mouse CD25 PerCP-Cya... View More
Product Details
50-5918-82
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
1TMYS
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574253
Product Specific Information
Description: This 1TMYS monoclonal antibody reacts with mouse Thymocyte-expressed molecule involved in selection (Themis), also known as Grb2-associating protein (Gasp). Themis is a cytoplasmic protein that is essential for positive selection and the development of single-positive thymocytes. Highest levels of expression are found in CD4+CD8+ double-positive thymocytes, with lower expression found in mature T cells. In addition to Grb1, Themis has been found to associate with PLC gamma 1 and LAT and is quickly phosphorylated by SLP-76 following TCR signaling.
There are 5 isoforms of mouse Themis that are produced as a result of alternative splicing. The 1TMYS antibody recognizes isoforms 1, 2, 3, and 4, but does not recognize isoform 5.
Applications Reported: This 1TMYS antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 1TMYS antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes or splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Themis is a cytoplasmic protein that is essential for positive selection and the development of single-positive thymocytes. Highest levels of expression are found in CD4+CD8+ double-positive thymocytes, with lower expression found in mature T cells. In addition to Grb1, Themis has been found to associate with PLC gamma 1 and LAT and is quickly phosphorylated by SLP-76 following TCR signaling. There are 5 isoforms of mouse Themis that are produced as a result of alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bA325O24.3; bA325O24.4; FLJ40584; Gasp; Grb2-associating protein; MGC163388; Protein THEMIS; Protein thylex; Thymocyte-expressed molecule involved in selection
Gene Aliases: E430004N04Rik; Gasp; Spot; Themis; thylex; Tsepa
UniProt ID: (Mouse) Q8BGW0
Entrez Gene ID: (Mouse) 210757
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