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Invitrogen
CD368 (Clec6) Monoclonal Antibody (9B9), eFluor™ 660, eBioscience™
Application
FIGURE: 1 / 1
CD368 (Clec6) Antibody (50-9113-41) in Flow
Normal human peripheral blood was incubated with the 1-step Fix/Lyse Solution (10X) (Product # 00-5333-54) and then stained with Anti-Human CD14 PE (Product # 12-0149-42), Anti-Human CD16 FITC (Product # 11-0168-42), and Mouse IgG2b K Isotype Control eFluor® 660 (Product # 50-4732-82) (blue histogram) or Anti-Human CD368 (Clec6) eFluor® 660 (purple histogram). Cells in the granulocyte (left) and monocyte (right) gates were used for analysis.
Product Details
50-9113-41
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
9B9
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574320
Product Specific Information
Description: This 9B9 monoclonal antibody reacts with human CD368, also known as Clec6 or ClecsF8 or Clec4D. In peripheral blood, this C-type lectin is expressed highly on granulocytes and CD14+CD16- monocytes. Some dendritic cell populations also exhibit low levels of CD368 expression. Studies have demonstrated that this receptor undergoes endocytosis due to its C-type lectin-like domain. CD368 functions as a phagocytic receptor and is involved in proinflammatory cytokine production in cooperation with Toll-like receptors. Finally, CD368 also participates in cellular activation via signaling through Syk kinase.
Applications Reported: This 9B9 antibody has been reported for use in flow cytometric analysis. Due to the endocytic nature of Clec6, we recommend fixing your cells prior staining to ensure the maximum possible signal.
Applications Tested: This 9B9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 8; C-type lectin domain family 4 member D; C-type lectin domain family 4, member D; C-type lectin receptor; C-type lectin superfamily member 8; C-type lectin-like receptor 6; CD368; CLEC-6; CLECSF8Mpcl; DC-associated C-type lectin 3; Dendritic cell-associated C-type lectin 3; macrophage C-type lectin; MGC40078; superfamilymember 8
Gene Aliases: CD368; CLEC-6; CLEC4D; CLEC6; CLECSF8; MCL; MPCL
UniProt ID: (Human) Q8WXI8
Entrez Gene ID: (Human) 338339
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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