Invitrogen

FOXP3 Monoclonal Antibody (236A/E7), Alexa Fluor™ 488, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

53 Published Figures

53 References

View all (163) FOXP3 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Published Figures (53)
Advanced Verification (1)

FOXP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 54

FOXP3 Antibody (53-4777-42) in IHC (P)

The Journal of infectious diseases 2010 - Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry (Paraffin)

FOXP3 Antibody in Immunohistochemistry (Frozen) (IHC (F))

FOXP3 Antibody in Immunohistochemistry (IHC)

FOXP3 Antibody in Western Blot, Immunoprecipitation (WB, IP)

FOXP3 Antibody in Radioimmune assays (RIA)

Product Image Gallery

FOXP3 Antibody — Intracellular staining of human peripheral blood cells. As expected based on known expression patterns, Foxp3 clone 236A/E7 stains CD4+CD25+ T cells and does not stain CD4- cells or CD4+CD25- T cells. Details: Normal human peripheral blood cells were surface stained with CD4 (clone OKT4) and CD25 (clone BC96) followed by intracellular staining with Foxp3 (clone 236A/E7) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD4- (left) and CD4+ (right) gates were used for analysis. {RE}

Product Details

53-4777-42

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 23 publications 23 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 24 publications 24 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Radioimmune Assays (RIA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate, Rhesus monkey

Published species

Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

236A/E7

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10804652

Product Specific Information

Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol reveals staining of the CD4+CD25bright population.

The epitope from 236A/E7 is different from that of PCH101 (Product # 72-5776-40). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.

Applications Reported: This 236A/E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis and IHC.

Applications Tested: This 236A/E7 antibody has been pre-titrated and tested using the Foxp3/Transctiption Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Changes of T Lymphocyte Subsets in Peripheral Blood of Patients with Intermediate and Advanced Cervical Cancer before and after Nimotuzumab Combined with Chemoradiotherapy.

International archives of allergy and immunology

Teng F,Cui G,Qian L,Zhao L

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Thu Jan 05 00:00:00 EST 2023

Tumor microenvironment shows an immunological abscopal effect in patients with NSCLC treated with pembrolizumab-radiotherapy combination.

Journal for immunotherapy of cancer

van der Woude LL,Gorris MAJ,Wortel IMN,Creemers JHA,Verrijp K,Monkhorst K,Grünberg K,van den Heuvel MM,Textor J,Figdor CG,Piet B,Theelen WSME,de Vries IJM

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Sat Oct 01 00:00:00 EDT 2022

Analysis of Expression of Different Histone Deacetylases in Autoimmune Thyroid Disease.

The Journal of clinical endocrinology and metabolism

Sacristán-Gómez P,Serrano-Somavilla A,González-Amaro R,Martínez-Hernández R,Marazuela M

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Thu Oct 21 00:00:00 EDT 2021

Common clonal origin of conventional T cells and induced regulatory T cells in breast cancer patients.

Nature communications

Xydia M,Rahbari R,Ruggiero E,Macaulay I,Tarabichi M,Lohmayer R,Wilkening S,Michels T,Brown D,Vanuytven S,Mastitskaya S,Laidlaw S,Grabe N,Pritsch M,Fronza R,Hexel K,Schmitt S,Müller-Steinhardt M,Halama N,Domschke C,Schmidt M,von Kalle C,Schütz F,Voet T,Beckhove P

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Thu Feb 18 00:00:00 EST 2021

Increased CD3⁺, CD8⁺, or FoxP3⁺ T Lymphocyte Infiltrations Are Associated with the Pathogenesis of Colorectal Cancer but Not with the Overall Survival of Patients.

Biology

Barbosa AM,Martinho O,Nogueira R,Campos J,Lobo L,Pinto H,Longatto-Filho A,Castro AG,Martins SF,Torrado E

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Fri Aug 20 00:00:00 EDT 2021

Multiple Immunostainings with Different Epitope Retrievals-The FOLGAS Protocol.

International journal of molecular sciences

von Schoenfeld A,Bronsert P,Poc M,Fuller A,Filby A,Kraft S,Kurowski K,Sörensen K,Huber J,Pfeiffer J,Proietti M,Stehl V,Werner M,Seidl M

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Sat Dec 25 00:00:00 EST 2021

Immune cell topography predicts response to PD-1 blockade in cutaneous T cell lymphoma.

Nature communications

Phillips D,Matusiak M,Gutierrez BR,Bhate SS,Barlow GL,Jiang S,Demeter J,Smythe KS,Pierce RH,Fling SP,Ramchurren N,Cheever MA,Goltsev Y,West RB,Khodadoust MS,Kim YH,Schürch CM,Nolan GP

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Thu Nov 18 00:00:00 EST 2021

Alterations in T and B cell function persist in convalescent COVID-19 patients.

Med (New York, N.Y.)

Shuwa HA,Shaw TN,Knight SB,Wemyss K,McClure FA,Pearmain L,Prise I,Jagger C,Morgan DJ,Khan S,Brand O,Mann ER,Ustianowski A,Bakerly ND,Dark P,Brightling CE,Brij S,Felton T,Simpson A,Grainger JR,Hussell T,Konkel JE,Menon M

53-4777-42 was used in Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) to investigate the long term effects of COVID-19 on T and B cells.

Fri Jun 11 00:00:00 EDT 2021

Muscle-Directed Delivery of an AAV1 Vector Leads to Capsid-Specific T Cell Exhaustion in Nonhuman Primates and Humans.

Molecular therapy : the journal of the American Society of Gene Therapy

Gernoux G,Gruntman AM,Blackwood M,Zieger M,Flotte TR,Mueller C

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Wed Mar 04 00:00:00 EST 2020

Mitochondrial transfer from MSCs to T cells induces Treg differentiation and restricts inflammatory response.

EMBO reports

Court AC,Le-Gatt A,Luz-Crawford P,Parra E,Aliaga-Tobar V,Bátiz LF,Contreras RA,Ortúzar MI,Kurte M,Elizondo-Vega R,Maracaja-Coutinho V,Pino-Lagos K,Figueroa FE,Khoury M

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Wed Feb 05 00:00:00 EST 2020

Single-cell multi-omics analysis presents the landscape of peripheral blood T-cell subsets in human chronic prostatitis/chronic pelvic pain syndrome.

Journal of cellular and molecular medicine

Zhang M,Liu Y,Chen J,Chen L,Meng J,Yang C,Yin S,Zhang X,Zhang L,Hao Z,Chen X,Liang C

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

hESC-derived immune suppressive dendritic cells induce immune tolerance of parental hESC-derived allografts.

EBioMedicine

Todorova D,Zhang Y,Chen Q,Liu J,He J,Fu X,Xu Y

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Tue Dec 01 00:00:00 EST 2020

Biomarker recommendation for PD-1/PD-L1 immunotherapy development in pediatric cancer based on digital image analysis of PD-L1 and immune cells.

The journal of pathology. Clinical research

Silva MA,Triltsch N,Leis S,Kanchev I,Tan TH,Van Peel B,Van Kerckhoven M,Deschoolmeester V,Zimmermann J

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Wed Apr 01 00:00:00 EDT 2020

Digital Pathology Analysis Quantifies Spatial Heterogeneity of CD3, CD4, CD8, CD20, and FoxP3 Immune Markers in Triple-Negative Breast Cancer.

Frontiers in physiology

Mi H,Gong C,Sulam J,Fertig EJ,Szalay AS,Jaffee EM,Stearns V,Emens LA,Cimino-Mathews AM,Popel AS

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Wed Nov 18 00:00:00 EST 2020

Distinct Tumor Microenvironments Are a Defining Feature of Strain-Specific CRISPR/Cas9-Induced MPNSTs.

Genes

Scherer A,Stephens VR,McGivney GR,Gutierrez WR,Laverty EA,Knepper-Adrian V,Dodd RD

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Sat May 23 00:00:00 EDT 2020

Inhibition of RANK signaling in breast cancer induces an anti-tumor immune response orchestrated by CD8+ T cells.

Nature communications

Gómez-Aleza C,Nguyen B,Yoldi G,Ciscar M,Barranco A,Hernández-Jiménez E,Maetens M,Salgado R,Zafeiroglou M,Pellegrini P,Venet D,Garaud S,Trinidad EM,Benítez S,Vuylsteke P,Polastro L,Wildiers H,Simon P,Lindeman G,Larsimont D,Van den Eynden G,Velghe C,Rothé F,Willard-Gallo K,Michiels S,Muñoz P,Walzer T,Planelles L,Penninger J,Azim HA,Loi S,Piccart M,Sotiriou C,González-Suárez E

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Thu Dec 10 00:00:00 EST 2020

Inhibitory activity of FOXP3+ regulatory T cells reveals high specificity for displaying immune tolerance in remission state rheumatoid arthritis.

Scientific reports

Kanjana K,Chevaisrakul P,Matangkasombut P,Paisooksantivatana K,Lumjiaktase P

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Fri Nov 13 00:00:00 EST 2020

Hijacking antibody-induced CTLA-4 lysosomal degradation for safer and more effective cancer immunotherapy.

Cell research

Zhang Y,Du X,Liu M,Tang F,Zhang P,Ai C,Fields JK,Sundberg EJ,Latinovic OS,Devenport M,Zheng P,Liu Y

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2019

Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer.

Scientific data

Rashid R,Gaglia G,Chen YA,Lin JR,Du Z,Maliga Z,Schapiro D,Yapp C,Muhlich J,Sokolov A,Sorger P,Santagata S

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Tue Dec 17 00:00:00 EST 2019

Molecular profiling of primary uveal melanomas with tumor-infiltrating lymphocytes.

Oncoimmunology

Triozzi PL,Schoenfield L,Plesec T,Saunthararajah Y,Tubbs RR,Singh AD

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Wed Feb 03 00:00:00 EST 2021

STAT3 Inhibition Combined with CpG Immunostimulation Activates Antitumor Immunity to Eradicate Genetically Distinct Castration-Resistant Prostate Cancers.

Clinical cancer research : an official journal of the American Association for Cancer Research

Moreira D,Adamus T,Zhao X,Su YL,Zhang Z,White SV,Swiderski P,Lu X,DePinho RA,Pal SK,Kortylewski M

53-4777 was used in Flow cytometry/Cell sorting to show a novel strategy combining STAT3 inhibition with Toll-like Receptor 9 (TLR9) stimulation to unleash immune response against prostate cancers regardless of the genetic background.

Sat Dec 01 00:00:00 EST 2018

Increased circulating regulatory T cells in medicated people with schizophrenia.

Psychiatry research

Kelly DL,Li X,Kilday C,Feldman S,Clark S,Liu F,Buchanan RW,Tonelli LH

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Thu Nov 01 00:00:00 EDT 2018

Spontaneous Regression of Ovarian Carcinoma After Septic Peritonitis; A Unique Case Report.

Frontiers in oncology

Roelofsen T,Wefers C,Gorris MAJ,Textor JC,Massuger LFAG,de Vries IJM,van Altena AM

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Wed Oct 04 00:00:00 EDT 2023

Multicomponent analysis of the tumour microenvironment reveals low CD8 T cell number, low stromal caveolin-1 and high tenascin-C and their combination as significant prognostic markers in non-small cell lung cancer.

Oncotarget

Onion D,Isherwood M,Shridhar N,Xenophontos M,Craze ML,Day LJ,García-Márquez MA,Pineda RG,Reece-Smith AM,Saunders JH,Duffy JP,Argent RH,Grabowska AM

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Fri Jan 05 00:00:00 EST 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.

Immunity

Kishore M,Cheung KCP,Fu H,Bonacina F,Wang G,Coe D,Ward EJ,Colamatteo A,Jangani M,Baragetti A,Matarese G,Smith DM,Haas R,Mauro C,Wraith DC,Okkenhaug K,Catapano AL,De Rosa V,Norata GD,Marelli-Berg FM

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Tue Nov 21 00:00:00 EST 2017

Cutting Edge: Selective Oral ROCK2 Inhibitor Reduces Clinical Scores in Patients with Psoriasis Vulgaris and Normalizes Skin Pathology via Concurrent Regulation of IL-17 and IL-10.

Journal of immunology (Baltimore, Md. : 1950)

Zanin-Zhorov A,Weiss JM,Trzeciak A,Chen W,Zhang J,Nyuydzefe MS,Arencibia C,Polimera S,Schueller O,Fuentes-Duculan J,Bonifacio KM,Kunjravia N,Cueto I,Soung J,Fleischmann RM,Kivitz A,Lebwohl M,Nunez M,Woodson J,Smith SL,West RF,Berger M,Krueger JG,Ryan JL,Waksal SD

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Radioimmune Assays

Mon May 15 00:00:00 EDT 2017

A novel method for assaying human regulatory T cell direct suppression of B cell effector function.

Journal of immunological methods

Weingartner E,Courneya JP,Keegan A,Golding A

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Tue Dec 26 00:00:00 EST 2017

Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment.

Oncoimmunology

Jiang X,Seo YD,Chang JH,Coveler A,Nigjeh EN,Pan S,Jalikis F,Yeung RS,Crispe IN,Pillarisetty VG

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Sat Nov 04 00:00:00 EDT 2023

Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment.

Oncotarget

Syed Khaja AS,Toor SM,El Salhat H,Faour I,Ul Haq N,Ali BR,Elkord E

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Tue May 16 00:00:00 EDT 2017

Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

The Journal of clinical investigation

MacDonald KG,Hoeppli RE,Huang Q,Gillies J,Luciani DS,Orban PC,Broady R,Levings MK

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2016

The immune microenvironment of breast ductal carcinoma in situ.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Thompson E,Taube JM,Elwood H,Sharma R,Meeker A,Warzecha HN,Argani P,Cimino-Mathews A,Emens LA

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Tue Mar 01 00:00:00 EST 2016

Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis.

International journal of molecular medicine

Liang DY,Hou YQ,Luo LJ,Ao L

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

The ratio of CD8 to Treg tumor-infiltrating lymphocytes is associated with response to cisplatin-based neoadjuvant chemotherapy in patients with muscle invasive urothelial carcinoma of the bladder.

Oncoimmunology

Baras AS,Drake C,Liu JJ,Gandhi N,Kates M,Hoque MO,Meeker A,Hahn N,Taube JM,Schoenberg MP,Netto G,Bivalacqua TJ

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Sun May 01 00:00:00 EDT 2016

Differential tumor infiltration by T-cells characterizes intrinsic molecular subtypes in breast cancer.

Journal of translational medicine

Miyan M,Schmidt-Mende J,Kiessling R,Poschke I,de Boniface J

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry (Frozen)

Fri Jul 29 00:00:00 EDT 2016

Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.

The Journal of biological chemistry

Ghosh S,Taylor A,Chin M,Huang HR,Conery AR,Mertz JA,Salmeron A,Dakle PJ,Mele D,Cote A,Jayaram H,Setser JW,Poy F,Hatzivassiliou G,DeAlmeida-Nagata D,Sandy P,Hatton C,Romero FA,Chiang E,Reimer T,Crawford T,Pardo E,Watson VG,Tsui V,Cochran AG,Zawadzke L,Harmange JC,Audia JE,Bryant BM,Cummings RT,Magnuson SR,Grogan JL,Bellon SF,Albrecht BK,Sims RJ,Lora JM

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunoprecipitation

Fri Jun 17 00:00:00 EDT 2016

Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.

The Journal of biological chemistry

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunoprecipitation

Fri Jun 17 00:00:00 EDT 2016

Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.

The Journal of biological chemistry

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunoprecipitation

Fri Jun 17 00:00:00 EDT 2016

Inhibition of the JAK/STAT Signaling Pathway in Regulatory T Cells Reveals a Very Dynamic Regulation of Foxp3 Expression.

PloS one

Goldstein JD,Burlion A,Zaragoza B,Sendeyo K,Polansky JK,Huehn J,Piaggio E,Salomon BL,Marodon G

53-4777 was used in Flow cytometry/Cell sorting to investigate the role of the JAK/STAT pathway in controlling Foxp3 in the short term, showing dynamic regulation of Foxp3 expression.

Thu Aug 25 00:00:00 EDT 2016

Changes in peripheral blood lymphocytes in polycythemia vera and essential thrombocythemia patients treated with pegylated-interferon alpha and correlation with JAK2^V617F allelic burden.

Experimental hematology & oncology

Kovacsovics-Bankowski M,Kelley TW,Efimova O,Kim SJ,Wilson A,Swierczek S,Prchal J

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunofluorescence

Tue Oct 20 00:00:00 EDT 2015

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunofluorescence

Tue Oct 20 00:00:00 EDT 2015

Altered expression of autoimmune regulator in infant down syndrome thymus, a possible contributor to an autoimmune phenotype.

Journal of immunology (Baltimore, Md. : 1950)

Skogberg G,Lundberg V,Lindgren S,Gudmundsdottir J,Sandström K,Kämpe O,Annerén G,Gustafsson J,Sunnegårdh J,van der Post S,Telemo E,Berglund M,Ekwall O

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Mon Sep 01 00:00:00 EDT 2014

Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma.

Oncotarget

Ye SB,Li ZL,Luo DH,Huang BJ,Chen YS,Zhang XS,Cui J,Zeng YX,Li J

53-4777 was used in Flow cytometry/Cell sorting to show that tumour-derived exosomes promote tumour progression and T-cell dysfunction through regulation of exosomal microRNAs in human carcinomas.

Wed Jul 30 00:00:00 EDT 2014

HLA Class II tetramers reveal tissue-specific regulatory T cells that suppress T-cell responses in breast carcinoma patients.

Oncoimmunology

Schmidt HH,Ge Y,Hartmann FJ,Conrad H,Klug F,Nittel S,Bernhard H,Domschke C,Schuetz F,Sohn C,Beckhove P

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2013

IDO and regulatory T cell support are critical for cytotoxic T lymphocyte-associated Ag-4 Ig-mediated long-term solid organ allograft survival.

Journal of immunology (Baltimore, Md. : 1950)

Sucher R,Fischler K,Oberhuber R,Kronberger I,Margreiter C,Ollinger R,Schneeberger S,Fuchs D,Werner ER,Watschinger K,Zelger B,Tellides G,Pilat N,Pratschke J,Margreiter R,Wekerle T,Brandacher G

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Sun Jan 01 00:00:00 EST 2012

Expansion of CD4+CD25+FOXP3+ regulatory T cells in infants of mothers with type 1 diabetes.

Pediatric diabetes

Luopajärvi K,Nieminen JK,Ilonen J,Akerblom HK,Knip M,Vaarala O

53-4777 was used in Flow cytometry/Cell sorting to suggest that maternal insulin treatment induces expansion of regulatory T-cells in the fetus.

Wed Aug 01 00:00:00 EDT 2012

CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.

The Journal of infectious diseases

Green AM,Mattila JT,Bigbee CL,Bongers KS,Lin PL,Flynn JL

Sun Aug 15 00:00:00 EDT 2010

Selective expression of latency-associated peptide (LAP) and IL-1 receptor type I/II (CD121a/CD121b) on activated human FOXP3+ regulatory T cells allows for their purification from expansion cultures.

Blood

Tran DQ,Andersson J,Hardwick D,Bebris L,Illei GG,Shevach EM

53-4777 was used in Flow cytometry/Cell sorting to create a protocol facilitating the purification of Tregs for cell-based therapies and detailed studies of human Treg function in health and disease.

Thu May 21 00:00:00 EDT 2009

Gamma c-signaling cytokines induce a regulatory T cell phenotype in malignant CD4+ T lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)

Kasprzycka M,Zhang Q,Witkiewicz A,Marzec M,Potoczek M,Liu X,Wang HY,Milone M,Basu S,Mauger J,Choi JK,Abrams JT,Hou JS,Rook AH,Vonderheid E,Woetmann A,Odum N,Wasik MA

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunohistochemistry

Fri Aug 15 00:00:00 EDT 2008

Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.

Journal of immunology (Baltimore, Md. : 1950)

Estes JD,Gordon SN,Zeng M,Chahroudi AM,Dunham RM,Staprans SI,Reilly CS,Silvestri G,Haase AT

Thu May 15 00:00:00 EDT 2008

IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients.

Blood

Ahmadzadeh M,Rosenberg SA

Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry

Wed Mar 15 00:00:00 EST 2006

Bioinformatics

Protein Aliases: Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; regulatory protein Foxp3; RP23-54C14.1; Scurfin

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Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; XPID

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UniProt ID: (Human) Q9BZS1

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Entrez Gene ID: (Human) 50943, (Rhesus monkey) 574303

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NF-kappaB binding NFAT protein binding

Process(es)

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FOXP3 Monoclonal Antibody (236A/E7), Alexa Fluor™ 488, eBioscience™

FOXP3 Antibody (53-4777-42) in IHC (P)

Product Details

53-4777-42

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Radioimmune Assays (RIA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

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Shipping conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

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