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Invitrogen

CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), PE-eFluor™ 610, eBioscience™

Name: CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), PE-eFluor™ 610, eBioscience™
Brand: Invitrogen
SKU: 61-9185-42
Price: 490254.0 CLP

7 References

View all (70) CXCR5 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)

Application

CD185 (CXCR5) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

CD185 (CXCR5) Antibody (61-9185-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG2b K Isotype Control PE-eFluor® 610 (Product # 61-4732-82) (left) or Anti-Human CD185 (CXCR5) PE-eFluor® 610 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

61-9185-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human, Non-human primate, Rhesus monkey

Published species

Human, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MU5UBEE

Immunogen

N-terminal extracellular domain

View immunogen

Conjugate

PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2574677

Product Specific Information

Description: The MU5UBEE monoclonal antibody reacts with human and non-human primate CD185. CD185, which is also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a seven transmembrane G protein-coupled receptor originally identified in Burkitt's lymphoma. In peripheral blood, CD185 is expressed on B cells, CD4+ T cells (but not Th1 or Th2 cells), as well as on a subpopulation of memory (CD45RO+) T cells. Circulating CD185+ T cells are in a resting state and migrate to the lymph nodes due to expression of CCR7 and CD62L. In tonsil, CD185 is expressed on nearly all CD4+ cells together with CD45RO and activation markers such as CD69 and ICOS. Tonsillar CD185+ cells have been shown to induce antibody production when co-cultured with B cells, thus supporting their role in providing help to B cells. Furthermore, this chemokine receptor plays a critical role in lymphocyte trafficking, in particular T cell migration into the B cell follicles of germinal centers in response to CXCL13, making CD185 an established marker of follicular helper T cells.

The MU5UBEE monoclonal antibody also crossreacts with Rhesus macaque.

Applications Reported: This MU5UBEE antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MU5UBEE antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD185, also known as CXCR5 or Burkitt lymphoma receptor 1 (BLR1), is a seven-transmembrane G protein-coupled receptor in the CXC chemokine receptor family. It plays a crucial role in lymphocyte trafficking, particularly in T cell migration into B cell follicles of germinal centers in response to CXCL13, making it a key marker of follicular helper T cells. In peripheral blood, CXCR5 is expressed on B cells, CD4+ T cells (excluding Th1 and Th2 cells), and a subset of memory (CD45RO+) T cells. These CD185+ T cells are in a resting state and migrate to lymph nodes due to CCR7 and CD62L expression. In tonsils, CD185 is found on nearly all CD4+ cells, along with activation markers like CD69 and ICOS, and can induce antibody production when co-cultured with B cells. CXCR5 is expressed in lymphatic tissues such as lymph node follicles and the spleen, and is also found in human blood and salivary glands, as well as various animal tissues. It is essential for B cell migration and is part of the larger family of G protein-coupled receptors (GPCRs), which have seven membrane-spanning helices and are involved in numerous signaling pathways. GPCRs, including CXCR5, are regulated by various ligands that affect their conformation and signaling through G-proteins and downstream effectors. The regulation of GPCRs, including their deactivation, has significant implications for cellular functions and is a major focus of pharmacological research, leading to the development of drugs like Zyprexa and Claritin for mental health and allergy treatments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

In Vivo Treatment with Insulin-like Growth Factor 1 Reduces CCR5 Expression on Vaccine-Induced Activated CD4⁺ T-Cells.

Vaccines

Bissa M,Galli V,Schifanella L,Vaccari M,Rahman MA,Gorini G,Binello N,Sarkis S,Gutowska A,Silva de Castro I,Doster MN,Moles R,Ferrari G,Shen X,Tomaras GD,Montefiori DC,N'guessan KF,Paquin-Proulx D,Kozlowski PA,Venzon DJ,Choo-Wosoba H,Breed MW,Kramer J,Franchini G

61-9185-42 was used in Flow Cytometry to find that IGF-1 changed the hierarchy of V1/V2 epitope recognition and decreased both ADCC specific for helical V2 and efferocytosis.

Mon Oct 30 00:00:00 UTC 2023

Vaccine plus microbicide effective in preventing vaginal SIV transmission in macaques.

Nature microbiology

Rahman MA,Bissa M,Silva de Castro I,Helmold Hait S,Stamos JD,Bhuyan F,Hunegnaw R,Sarkis S,Gutowska A,Doster MN,Moles R,Hoang T,Miller Jenkins LM,Appella E,Venzon DJ,Choo-Wosoba H,Cardozo T,Baum MM,Appella DH,Robert-Guroff M,Franchini G

61-9185-42 was used in Flow cytometry/Cell sorting to show that, by increasing zinc availability, SAMT-247 increases natural killer cytotoxicity and monocyte efferocytosis, and decreases T-cell activation to augment vaccine-induced protection.

Mon May 01 00:00:00 UTC 2023

Phenotypic and functional characterization of pharmacologically expanded Vγ9Vδ2 T cells in pigtail macaques.

iScience

Barber-Axthelm IM,Wragg KM,Esterbauer R,Amarasena TH,Barber-Axthelm VRB,Wheatley AK,Gibbon AM,Kent SJ,Juno JA

61-9185-42 was used in Flow cytometry/Cell sorting to show the tissue distribution and phenotype of in vivo pharmacologically expanded Vγ9Vδ2 T cells can be altered based on the antigen administration route, with implications for tissue trafficking and the clinical efficacy of Vγ9Vδ2 T cell immunotherapeutics.

Fri Mar 17 00:00:00 UTC 2023

Spike-specific circulating T follicular helper cell and cross-neutralizing antibody responses in COVID-19-convalescent individuals.

Nature microbiology

Zhang J,Wu Q,Liu Z,Wang Q,Wu J,Hu Y,Bai T,Xie T,Huang M,Wu T,Peng D,Huang W,Jin K,Niu L,Guo W,Luo D,Lei D,Wu Z,Li G,Huang R,Lin Y,Xie X,He S,Deng Y,Liu J,Li W,Lu Z,Chen H,Zeng T,Luo Q,Li YP,Wang Y,Liu W,Qu X

61-9185 was used in Flow cytometry/Cell sorting to provide insight into neutralizing antibody responses in COVID-19-convalescent individuals and facilitate the treatment and vaccine development for SARS-CoV-2 infection.

Fri Jan 01 00:00:00 UTC 2021

HIV vaccine candidate activation of hypoxia and the inflammasome in CD14⁺ monocytes is associated with a decreased risk of SIV_mac251 acquisition.

Nature medicine

Vaccari M,Fourati S,Gordon SN,Brown DR,Bissa M,Schifanella L,Silva de Castro I,Doster MN,Galli V,Omsland M,Fujikawa D,Gorini G,Liyanage NPM,Trinh HV,McKinnon KM,Foulds KE,Keele BF,Roederer M,Koup RA,Shen X,Tomaras GD,Wong MP,Munoz KJ,Gach JS,Forthal DN,Montefiori DC,Venzon DJ,Felber BK,Rosati M,Pavlakis GN,Rao M,Sekaly RP,Franchini G

61-9185 was used in Flow cytometry/Cell sorting to examine the ability of several vaccine prime modalities together with two ALVAC-SIV + gp120 protein boosts to reduce the risk of SIVmac251 acquisition in Rhesus macaques.

Fri Jun 01 00:00:00 UTC 2018

Perturbed CD8⁺ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals.

Scientific reports

Tauriainen J,Scharf L,Frederiksen J,Naji A,Ljunggren HG,Sönnerborg A,Lund O,Reyes-Terán G,Hecht FM,Deeks SG,Betts MR,Buggert M,Karlsson AC

61918542 was used in flow cytometry to show T cell immunoglobulin and ITIM domain expression increases over time despite early initiation of antiretroviral treatment

Fri Jan 13 00:00:00 UTC 2017

Regulatory and Helper Follicular T Cells and Antibody Avidity to Simian Immunodeficiency Virus Glycoprotein 120.

Journal of immunology (Baltimore, Md. : 1950)

Blackburn MJ,Zhong-Min M,Caccuri F,McKinnon K,Schifanella L,Guan Y,Gorini G,Venzon D,Fenizia C,Binello N,Gordon SN,Miller CJ,Franchini G,Vaccari M

61-9185 was used in Flow cytometry/Cell sorting to demonstrate changes in the TFH/TFR ratio during chronic infection and suggest possible mechanisms for the unchecked expansion of TFH cells in HIV/SIV infection.

Thu Oct 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Blr-1; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 5; CC motif chemokine; CCmotif chemokine; CD185; CD185 antigen; chemokine (C-X-C motif) receptor 5; CXC; CXC motif chemokine; CXC-R5; CXCR-5; Fusin; GPR9; HUMSTR; LESTR; MDR-15; Monocyte-derived receptor 15

Gene Aliases: BLR1; CD185; CXCR5; EGK_06966; MDR15

UniProt ID: (Human) P32302

Entrez Gene ID: (Rhesus monkey) 701792, (Human) 643

Function(s)

Process(es)

movement of cell or subcellular component chemotaxis immune response G-protein coupled receptor signaling pathway positive regulation of cytokinesis B cell activation lymph node development chemokine-mediated signaling pathway

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