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            • Primary Antibodies ›
            • CD8 Antibodies

            Invitrogen

            CD8a Monoclonal Antibody (53-6.7), Super Bright™ 645, eBioscience™

            98 Published Figures
            99 References
            View all (611) CD8 antibodies
            Datasheet
            Protocols
            Questions & Answers
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            Catalog # 64-0081-80
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            Cite CD8a Monoclonal Antibody (53-6.7), Super Bright™ 645, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (98)
            Application
            CD8a Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 99

            CD8a Antibody (64-0081-80) in Flow

            Staining of mouse splenocytes with Anti-Mouse CD3 PE (Product # 12-0032) and 0.25 µg of Rat IgG2a K Isotype Control Super Bright 645 (Product # 64-4321-82) (left) or 0.25 µg of Anti-Mouse CD8a Super Bright 645 (right). Cells in the lymphocyte gate were used for analysis.
            View Product

            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)

            Product Details

            64-0081-80

            Applications
            Tested Dilution
            Publications

            Immunohistochemistry (IHC)

            -
            View 11 publications 11 publications

            Flow Cytometry (Flow)

            0.5 µg/test
            View 88 publications 88 publications
            Product Specifications

            Species Reactivity

            Mouse

            Published species

            Mouse

            Host/Isotype

            Rat / IgG2a, kappa

            Recommended Isotype Control

            Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 645, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            53-6.7

            Conjugate

            Super Bright™ 645
            • Unconjugated
            • Alexa Fluor 488 
            • Alexa Fluor 532 
            • Alexa Fluor 561
            • Alexa Fluor 660 
            • Alexa Fluor 700 
            • APC 
            • APC-eFluor 780
            • Biotin 
            • Brilliant UV 395
            • Brilliant UV 496
            • Brilliant UV 563
            • Brilliant UV 615
            • Brilliant UV 661
            • Brilliant UV 737
            • Brilliant UV 805
            • Brilliant Violet 421
            • Brilliant Violet 480
            • Brilliant Violet 605
            • Brilliant Violet 650
            • Brilliant Violet 711
            • Brilliant Violet 786
            • eFluor 450
            • eFluor 506
            • eFluor 615
            • eFluor 660
            • FITC
            • Functional Grade
            • NovaFluor Blue 510
            • NovaFluor Blue 585
            • NovaFluor Blue 610-70S
            • NovaFluor Blue 660-120S
            • NovaFluor Blue 725
            • NovaFluor Blue 760
            • NovaFluor Blue 800
            • NovaFluor Red 710
            • NovaFluor UV 765
            • NovaFluor Violet 690
            • NovaFluor Violet 745
            • NovaFluor Violet 800
            • NovaFluor Yellow 590
            • NovaFluor Yellow 610
            • NovaFluor Yellow 660
            • NovaFluor Yellow 690
            • NovaFluor Yellow 700
            • NovaFluor Yellow 730
            • NovaFluor Yellow 810
            • PE 
            • PE-Cyanine5
            • PE-Cyanine5.5
            • PE-Cyanine7
            • PE-eFluor 610
            • PerCP-Cyanine5.5
            • PerCP-eFluor 710
            • Super Bright 436
            • Super Bright 600
            • Super Bright 702
            • Super Bright 780
            • Request custom conjugation

            Excitation/Emission Max

            414/645 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.2 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2, with BSA

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2662352

            Product Specific Information

            Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

            Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis and immunohistology staining of frozen tissue sections.

            Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

            When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

            Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.

            Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

            Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

            Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

            Target Information

            Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            Bioinformatics

            Protein Aliases: CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3

            View more View less

            Gene Aliases: BB154331; Cd8a; Cd8b; Cd8b1; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; Lyt-2; Lyt-3; Lyt2; Lyt3

            View more View less

            UniProt ID: (Mouse) P01731, (Mouse) P10300

            View more View less

            Entrez Gene ID: (Mouse) 12525, (Mouse) 12526

            View more View less

            Function(s)

            protein binding protein kinase binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

            Process(es)

            immune system process T cell mediated immunity cell surface receptor signaling pathway T cell activation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus
            It has to be done as per old AB suggested Products section.
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