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            • Primary Antibodies ›
            • CD11b Antibodies

            Invitrogen

            CD11b Monoclonal Antibody (M1/70), Super Bright™ 645, eBioscience™

            97 Published Figures
            149 References
            View all (253) CD11b antibodies
            Datasheet
            Protocols
            Questions & Answers
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            Catalog # 64-0112-82
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            Cite CD11b Monoclonal Antibody (M1/70), Super Bright™ 645, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (97)
            Application
            CD11b Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 98

            CD11b Antibody (64-0112-82) in Flow

            Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2b K Isotype Control Super Bright 645 (Product # 64-4031-82) (left) or 0.06 µg of Anti-Mouse CD11b Super Bright 645 (right). Cells in the large scatter population were used for analysis.
            View Product

            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Immunohistochemistry (IHC)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Immunocytochemistry (ICC/IF)
            CD11b Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed) (ICC/IF, IHC (PFA))
            CD11b Antibody in Immunocytochemistry (ICC/IF)
            CD11b Antibody in Immunocytochemistry (ICC/IF)
            CD11b Antibody in Immunocytochemistry (ICC/IF)
            CD11b Antibody in Immunocytochemistry (ICC/IF)
            CD11b Antibody in Immunocytochemistry (ICC/IF)
            CD11b Antibody in Immunohistochemistry (Frozen) (IHC (F))
            CD11b Antibody in Immunohistochemistry (Frozen) (IHC (F))
            CD11b Antibody in Immunohistochemistry (IHC)
            CD11b Antibody in Immunohistochemistry (IHC)
            CD11b Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
            CD11b Antibody in Immunohistochemistry (IHC)
            CD11b Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
            CD11b Antibody in Immunohistochemistry (IHC)
            CD11b Antibody in Immunohistochemistry (IHC)
            CD11b Antibody in Immunohistochemistry (IHC)
            CD11b Antibody in Immunohistochemistry (IHC)
            CD11b Antibody in Immunohistochemistry (IHC)
            CD11b Antibody in Immunohistochemistry (IHC)
            CD11b Antibody in Immunohistochemistry (IHC)
            CD11b Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)

            Product Details

            64-0112-82

            Applications
            Tested Dilution
            Publications

            Immunohistochemistry (IHC)

            -
            View 13 publications 13 publications

            Immunohistochemistry (PFA fixed) (IHC (PFA))

            -
            View 4 publications 4 publications

            Immunohistochemistry (Frozen) (IHC (F))

            -
            View 2 publications 2 publications

            Immunocytochemistry (ICC/IF)

            -
            View 6 publications 6 publications

            Flow Cytometry (Flow)

            0.125 µg/test
            View 122 publications 122 publications

            Neutralization (Neu)

            -
            View 2 publications 2 publications
            Product Specifications

            Species Reactivity

            Mouse

            Published species

            Mouse

            Host/Isotype

            Rat / IgG2b, kappa

            Recommended Isotype Control

            Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 645, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            M1/70

            Conjugate

            Super Bright™ 645
            • Unconjugated
            • Alexa Fluor 488 
            • Alexa Fluor 532 
            • Alexa Fluor 660 
            • Alexa Fluor 700 
            • APC 
            • APC-eFluor 780
            • Biotin 
            • Brilliant UV 395
            • Brilliant UV 496
            • Brilliant UV 563
            • Brilliant UV 615
            • Brilliant UV 661
            • Brilliant UV 737
            • Brilliant UV 805
            • Brilliant Violet 421
            • Brilliant Violet 480
            • Brilliant Violet 605
            • Brilliant Violet 650
            • Brilliant Violet 711
            • Brilliant Violet 786
            • eFluor 450
            • eFluor 506
            • eFluor 660
            • FITC
            • Functional Grade
            • NovaFluor Blue 510
            • NovaFluor Blue 585
            • NovaFluor Blue 610-30S
            • NovaFluor Blue 660-40S
            • NovaFluor Yellow 570
            • NovaFluor Yellow 590
            • NovaFluor Yellow 660
            • PE 
            • PE-Cyanine5
            • PE-Cyanine7
            • PE-eFluor 610
            • PerCP-Cyanine5.5
            • PerCP-eFluor 710
            • Super Bright 436
            • Super Bright 600
            • Super Bright 702
            • Super Bright 780
            • Request custom conjugation

            Excitation/Emission Max

            414/645 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.2 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2, with BSA

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2662387

            Product Specific Information

            Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaM-beta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.

            Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis.

            Applications Tested: This M1/70 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

            When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

            Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.

            Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

            Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

            Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

            Target Information

            CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            Bioinformatics

            Protein Aliases: CD11 antigen-like family member B; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; integrin alpha-M; leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; MGC117044

            View more View less

            Gene Aliases: Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1

            View more View less

            Entrez Gene ID: (Mouse) 16409

            View more View less

            Function(s)

            opsonin binding glycoprotein binding heparin binding heparan sulfate proteoglycan binding metal ion binding protein heterodimerization activity integrin

            Process(es)

            leukocyte migration involved in inflammatory response cell adhesion leukocyte cell-cell adhesion integrin-mediated signaling pathway microglia development single organismal cell-cell adhesion neutrophil chemotaxis cellular extravasation activated T cell proliferation
            It has to be done as per old AB suggested Products section.
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