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Application
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RAGE Antibody (710339) in WB
Western blot analysis of RAGE in whole cell extracts from HepG2 (lane 1), Jurkat (lane2), and MDAMB-231 (lane3) cells using a RAGE Recombinant Rabbit Superclonal™ Antibody (Product # 710339) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~32kDa.
Product Details
710339
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
17HCLC
Immunogen
Recombinant protein corresponding to amino acids 23-342 of human sRAGE
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532692
Product Specific Information
This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
The Receptor for Advanced Glycation End-products (RAGE) is a gene located on human chromosome 6p21.3, encoding a transmembrane receptor belonging to the immunoglobulin superfamily. RAGE is expressed in various tissues, with significant levels in the lungs, and plays a crucial role in cellular signaling and inflammation. As a receptor, RAGE binds multiple ligands, including advanced glycation end-products (AGEs), amyloid-ß peptide, high mobility group box 1 (HMGB1), and S100/calgranulin proteins, facilitating diverse pathological processes like inflammation, cancer progression, and neurodegeneration. The interaction between RAGE and its ligands triggers intracellular signaling pathways such as NF-?B activation, leading to inflammatory responses and oxidative stress. In the context of chronic diseases like diabetes, Alzheimer's, and cardiovascular diseases, RAGE is a critical mediator, linking metabolic disturbance to cellular dysfunction. Therapeutic targeting of RAGE signaling is under investigation, aiming to mitigate its contribution to inflammatory and degenerative diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Advanced glycosylation end product-specific receptor; RAGE isoform NtRAGE-delta; RAGE isoform sRAGE-delta; RAGE-4 ORF3; receptor for advanced glycation end-products variant 20; Receptor for advanced glycosylation end products; sRAGE
Gene Aliases: AGER; RAGE; SCARJ1
UniProt ID: (Human) Q15109
Entrez Gene ID: (Human) 177
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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