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Invitrogen
Chicken anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647
Application
FIGURE: 1 / 7
Rat IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21472) in ICC/IF
Immunofluorescence analysis of Chicken anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (Product # A-21472) was performed using NTERA-2 (positive model) and Hela (negative model) in cells stained with SOX2 Monoclonal Antibody (Btjce), eBioscience (Product # 14-9811-82). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, b... View More
Product Details
A-21472
Applications
Tested Dilution
Western Blot (WB)
1:5,000-1:10,000
Immunohistochemistry (Paraffin) (IHC (P))
1-10 µg/mL
Immunocytochemistry (ICC/IF)
1:1,000-1:2,000
Product Specifications
Species Reactivity
Rat
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobins Heavy and Light chains
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
2 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.5
Contains
5mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2535875
Target
IgG
Cross Adsorption
Against human and rabbit IgG prior to conjugation
Antibody Form
Whole Antibody
Product Specific Information
Chicken anti-rat antibodies react with IgG heavy chains and all classes of immunoglobulin light chains from rat. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic ″Fc″ domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors. Fluorescence of this long-wavelength Alexa Fluor dye is not visible by looking through a conventional fluorescence microscope.
Product will be shipped at Room Temperature.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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