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Invitrogen

CD11b Monoclonal Antibody (M1/70), APC-Cyanine7

Name: CD11b Monoclonal Antibody (M1/70), APC-Cyanine7
Brand: Invitrogen
SKU: A15390
Price: 366.0 USD
Availability: InStock

1 Published Figure

8 References

View all (253) CD11b antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (1)

Application

FIGURE: 1 / 4

CD11b Antibody (A15390) in Flow

Flow cytometry analysis of CD11b in BALB/c mouse splenocytes. Cells were stained using CD11b Monoclonal Antibody (M1/70), APC-Cyanine7 (Product # A15390) and Rat Anti-Mouse Ly-6G/Ly-6C-APC.

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Product Details

A15390

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.3 µg/1x10^6 cells

View 8 publications 8 publications

Product Specifications

Species Reactivity

Baboon, Human, Mouse, Rabbit, Rhesus monkey

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

M1/70

Immunogen

C57BL/10 splenic T cells and concanavalin A stimulated C57BL/10 splenocytes.

View immunogen

Conjugate

APC-Cyanine7

Excitation/Emission Max

653/782 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.1, with proprietary stabilizer

Contains

<0.1% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2534404

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

GDF15 promotes weight loss by enhancing energy expenditure in muscle.

Nature

Wang D,Townsend LK,DesOrmeaux GJ,Frangos SM,Batchuluun B,Dumont L,Kuhre RE,Ahmadi E,Hu S,Rebalka IA,Gautam J,Jabile MJT,Pileggi CA,Rehal S,Desjardins EM,Tsakiridis EE,Lally JSV,Juracic ES,Tupling AR,Gerstein HC,Paré G,Tsakiridis T,Harper ME,Hawke TJ,Speakman JR,Blondin DP,Holloway GP,Jørgensen SB,Steinberg GR

A15390 was used in Flow cytometry/Cell sorting to indicate that therapeutic targeting of the GDF15-GFRAL pathway may be useful for maintaining energy expenditure in skeletal muscle during caloric restriction.

Sat Jul 01 00:00:00 UTC 2023

GPR84 regulates pulmonary inflammation by modulating neutrophil functions.

Acta pharmacologica Sinica

Wang SW,Zhang Q,Lu D,Fang YC,Yan XC,Chen J,Xia ZK,Yuan QT,Chen LH,Zhang YM,Nan FJ,Xie X

Published figure using CD11b monoclonal antibody (Product # A15390) in Flow Cytometry

Tue Aug 01 00:00:00 UTC 2023

GPR84 regulates pulmonary inflammation by modulating neutrophil functions.

Acta pharmacologica Sinica

Wang SW,Zhang Q,Lu D,Fang YC,Yan XC,Chen J,Xia ZK,Yuan QT,Chen LH,Zhang YM,Nan FJ,Xie X

Published figure using CD11b monoclonal antibody (Product # A15390) in Flow Cytometry

Tue Aug 01 00:00:00 UTC 2023

IRE1α drives lung epithelial progenitor dysfunction to establish a niche for pulmonary fibrosis.

American journal of physiology. Lung cellular and molecular physiology

Auyeung VC,Downey MS,Thamsen M,Wenger TA,Backes BJ,Sheppard D,Papa FR

A15390 was used in Flow cytometry/Cell sorting to show that the bifunctional kinase/RNase-IRE1α-a central mediator of the unfolded protein response (UPR) to endoplasmic reticulum (ER) stress is a critical promoter of DATP abundance and function.

Fri Apr 01 00:00:00 UTC 2022

Mesenchymal stem/stromal cells primed by inflammatory cytokines alleviate psoriasis-like inflammation via the TSG-6-neutrophil axis.

Cell death & disease

Ding Y,Gong P,Jiang J,Feng C,Li Y,Su X,Bai X,Xu C,Liu C,Yang J,Fang J,Ji X,Chen Y,Li P,Guo L,Shao C,Shi Y

A15390 was used in Flow cytometry/Cell sorting to find that subcutaneous application of umbilical cord-derived MSCs-IT exhibit remarkable TSG-6-mediated therapeutic efficacy on imiquimod (IMQ)-induced psoriasis-like inflammation in mice.

Fri Nov 25 00:00:00 UTC 2022

Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.

Frontiers in immunology

Agalave NM,Mody PH,Szabo-Pardi TA,Jeong HS,Burton MD

Published figure using CD11b monoclonal antibody (Product # A15390) in Flow Cytometry

Mon Sep 20 00:00:00 UTC 2021

Isolation, culture, and downstream characterization of primary microglia and astrocytes from adult rodent brain and spinal cord.

Journal of neuroscience methods

Agalave NM,Lane BT,Mody PH,Szabo-Pardi TA,Burton MD

A15390 was used in Flow Cytometry to show a novel, single protocol to isolate microglia and astrocytes from brain and spinal cord tissue, allowing for culturing and other downstream applications from the cells of animals of various ages, which will be useful for researchers investigating these two major glial cell types from the brain or spinal cord of the same rodent.

Wed Jul 01 00:00:00 UTC 2020

Autophagy promotes growth of tumors with high mutational burden by inhibiting a T-cell immune response.

Nature cancer

Poillet-Perez L,Sharp DW,Yang Y,Laddha SV,Ibrahim M,Bommareddy PK,Hu ZS,Vieth J,Haas M,Bosenberg MW,Rabinowitz JD,Cao J,Guan JL,Ganesan S,Chan CS,Mehnert JM,Lattime EC,White E

A15390 was used in Flow Cytometry to study autophagy promotion of tumor immune tolerance in regulating T-cell function and limiting interferon gene stimulation.

Tue Sep 01 00:00:00 UTC 2020

Autophagy maintains tumour growth through circulating arginine.

Nature

Poillet-Perez L,Xie X,Zhan L,Yang Y,Sharp DW,Hu ZS,Su X,Maganti A,Jiang C,Lu W,Zheng H,Bosenberg MW,Mehnert JM,Guo JY,Lattime E,Rabinowitz JD,White E

A15390 was used in Flow cytometry/Cell sorting to show that host-specific deletion of Atg7 impairs the growth of multiple allografted tumours but not all.

Thu Nov 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; Integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); Leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; macrophage antigen alpha polypeptide; MGC117044; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit

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Gene Aliases: CD11B; CD11b/CD18; CR3; CR3A; F730045J24Rik; ITGAM; Ly-40; MAC-1; Mac-1a; MAC1; MAC1A; MO1A; SLEB6

UniProt ID: (Human) P11215

Entrez Gene ID: (Human) 3684, (Rhesus monkey) 714832, (Mouse) 16409, (Rabbit) 100351865

Function(s)

glycoprotein binding protein binding metal ion binding opsonin binding heparin binding heparan sulfate proteoglycan binding protein heterodimerization activity integrin

Process(es)

cell adhesion integrin-mediated signaling pathway ectodermal cell differentiation extracellular matrix organization toll-like receptor 4 signaling pathway leukocyte migration leukocyte migration involved in inflammatory response leukocyte cell-cell adhesion microglia development single organismal cell-cell adhesion neutrophil chemotaxis cellular extravasation activated T cell proliferation

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CD11b Monoclonal Antibody (M1/70), APC-Cyanine7

CD11b Antibody (A15390) in Flow

Product Details

A15390

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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