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Application
FIGURE: 1 / 1
ROBO1 Antibody (A301-266A) in WB
Detection of human ROBO1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ROBO1 antibody (Product # A301-266A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. ROBO1 was also immunoprecipitated by rabbit anti-ROBO1 antibody A301-265A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).
Product Details
A301-266A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1601 and 1651 of Human roundabout, axon guidance receptor, homolog 1.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deleted in U twenty twenty; FLJ21882; H-Robo-1; HGNC:10249; MGC131599; MGC133277; roundabout 1; Roundabout homolog 1; roundabout, axon guidance receptor, homolog 1
Gene Aliases: DUTT1; ROBO1; SAX3
UniProt ID: (Human) Q9Y6N7
Entrez Gene ID: (Human) 6091
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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