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CD53 Antibody (MA1-10248) in Flow
Flow Cytometry analysis of CD53 using CD53 Monoclonal Antibody (MEM-53), PE (Product # MA1-10248). Separation of human monocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD53 PE antibody (20 μl reagent / 100 μl of peripheral whole blood).
Product Details
MA1-10248
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MEM-53
Immunogen
Leukocytes of pacient suffering from a LGL-type leukemia.
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11152343
Product Specific Information
This antibody reacts with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Target Information
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumor cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antigen MOX44 identified by monoclonal antibody MRC-OX44; CD53; CD53 antigen; CD53 glycoprotein; CD53 tetraspan antigen; cell surface antigen; Cell surface glycoprotein CD53; Leukocyte surface antigen CD53; Tetraspanin-25; transmembrane glycoprotein; Tspan-25
Gene Aliases: CD53; MOX44; TSPAN25
UniProt ID: (Human) P19397
Entrez Gene ID: (Human) 963
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