Invitrogen

CD4/CD28 Antibody Cocktail, FITC, PE

View all (3) CD4/CD28 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-12122

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Cocktail

Type

Antibody

Clone

7E14, B-23

Immunogen

human peripheral blood T lymphocytes and HPB-ALL T-cell line

Conjugate

FITC , PE FITC , PE FITC , PE

Form

Liquid

Purification

Protein A/G

Storage buffer

PBS with 0.2% carrier protein

Contains

0.08% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1072293

Product Specific Information

This antibody is a monoclonal mixture of FITC-conjugated CD4 (clone 7E14, isotype IgG1) and PE-conjugated CD28 (clone B-23, isotype IgG1).

Target Information

The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD28; CD28 antigen; CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; MGC138290; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell-specific surface glycoprotein CD28; TP44

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Gene Aliases: CD28; CD4; CD4mut; Tp44

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UniProt ID: (Human) P10747, (Human) P01730

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Entrez Gene ID: (Human) 940, (Human) 920

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Function(s)
protease binding SH3/SH2 adaptor activity protein binding coreceptor activity protein kinase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent zinc ion binding immunoglobulin binding enzyme binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding immunoglobulin receptor superfamily
Process(es)
positive regulation of inflammatory response to antigenic stimulus humoral immune response cell surface receptor signaling pathway positive regulation of gene expression negative regulation of gene expression regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling T cell costimulation positive regulation of interleukin-10 production positive regulation of interleukin-4 production cytokine biosynthetic process positive regulation of T cell proliferation negative thymic T cell selection regulatory T cell differentiation positive regulation of viral genome replication positive regulation of interleukin-2 biosynthetic process regulation of regulatory T cell differentiation positive regulation of translation positive regulation of mitotic nuclear division positive regulation of transcription from RNA polymerase II promoter positive regulation of alpha-beta T cell proliferation phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of isotype switching to IgG isotypes regulation of defense response to virus by virus T cell receptor signaling pathway positive regulation of protein kinase B signaling apoptotic signaling pathway cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response T cell selection positive regulation of protein kinase activity positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation
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