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Invitrogen
Cyclin B1 Monoclonal Antibody (V152)
Application
FIGURE: 1 / 16
Cyclin B1 Antibody (MA1-155) in ICC/IF
Immunofluorescent analysis of Cyclin B1 (green) showing staining in the in the cytoplasm and nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cyclin B1 monoclonal antibody (Product # MA1-1... View More
Product Details
MA1-155
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Non-human primate,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
V152
Immunogen
Hamster Cyclin B1
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 30% glycerol, 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536863
Product Specific Information
MA1-155 detects Cyclin B1 in mammalian samples. MA1-155 has been successfully used in Immunohistochemistry (paraffin), Immunofluorescence, Immunoprecipitation, Flow Cytometry and Western Blot procedures. Immunoprecipitation and Western Blot analysis with MA1-155 show the accumulation of a prominent band at ~52 kDa in camptothecin and hydroxyurea treated cells. MA1-155 also detects additional unknown bands at ~35 and ~75 kDa. In Immunofluorescence applications, MA1-155 shows cyclin B1 co-localization with microtubules, whereas MA1-156 shows cyclin B2 staining consistent with the Golgi region.
MA1-155 specifically detects Cyclin B1 in in whole cell lysates, but will also cross-react with purified recombinant human Cyclin B2 in Western Blot applications.
Target Information
Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cyclin B1; cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; OTTHUMP00000221981; OTTHUMP00000223023
Gene Aliases: Ccn-2; CCNB; CCNB1; Ccnb1-rs1; Ccnb1-rs13; Cycb; Cycb-4; Cycb-5; CycB1; Cycb1-rs1; I79_013589
UniProt ID: (Human) P14635, (Chinese hamster) Q08301, (Rat) P30277, (Mouse) P24860
Entrez Gene ID: (Human) 891, (Chinese hamster) 100770842, (Rat) 25203, (Mouse) 268697
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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