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            • Primary Antibodies ›
            • CD45 Antibodies

            Invitrogen

            CD45 Monoclonal Antibody (MEM-28), PE

            View all (444) CD45 antibodies
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            Catalog # MA1-19635
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            Price (USD)
            467.00
            100 Tests
             
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            Cite CD45 Monoclonal Antibody (MEM-28), PE

            • Antibody Testing Data (2)
            Application
            CD45 Antibody in Flow Cytometry (Flow)
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            CD45 Antibody (MA1-19635) in Flow

            Separation of human lymphocytes (red-filled) from human CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD45 (MEM-28) PE Monoclonal antibody (Product # MA1-19635) using a dilution of 20 µL reagent/100 µL of peripheral whole blood.
            View Product

            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)

            Product Details

            MA1-19635

            Applications
            Tested Dilution
            Publications

            Flow Cytometry (Flow)

            20 µL/1x10^6 cells
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Mouse / IgG1

            Class

            Monoclonal

            Type

            Antibody

            Clone

            MEM-28

            Immunogen

            Human thymocytes and T lymphocytes.
            View immunogen

            Conjugate

            PE
            • Unconjugated
            • Alexa Fluor 488 
            • Alexa Fluor 647 
            • Alexa Fluor 700 
            • APC 
            • Biotin 
            • FITC
            • PerCP 
            • Request custom conjugation

            Excitation/Emission Max

            565/576 nm View spectra spectra

            Form

            Liquid

            Purification

            Size-exclusion chromatography

            Storage buffer

            PBS, pH 7.4, with 0.2% BSA

            Contains

            15mM sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_1072431

            Product Specific Information

            This antibody reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. This antibody will not cross-react with equine.

            Target Information

            CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            Cite this product

            Bioinformatics

            Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

            View more View less

            Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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            UniProt ID: (Human) P08575

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            Entrez Gene ID: (Human) 5788

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            Function(s)

            protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

            Process(es)

            negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation
            It has to be done as per old AB suggested Products section.
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