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            • Primary Antibodies ›
            • EpCAM (CD326) Antibodies

            Invitrogen

            EpCAM Monoclonal Antibody (323/A3)

            Advanced Verification
            7 Published Figures
            19 References
            View all (278) EpCAM (CD326) antibodies
            Datasheet
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            Catalog # MA5-12436
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            Price (USD)
            546.00
            500 µL
             
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            Datasheet
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            Cite EpCAM Monoclonal Antibody (323/A3)

            • Antibody Testing Data (6)
            • Published Figures (7)
            • Advanced Verification (1)
            Application
            EpCAM Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 14

            EpCAM Antibody (MA5-12436) in ICC/IF

            Immunofluorescence analysis of EpCAM was performed using HT-29 and BJ cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EpCAM Mouse Monoclonal Antibody (Product # MA5-12436) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A28175) at a dilution of 1:... View More
            View Product

            EpCAM Antibody in Immunocytochemistry (ICC/IF)
            EpCAM Antibody in Immunocytochemistry (ICC/IF)
            EpCAM Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            EpCAM Antibody in Flow Cytometry (Flow)
            EpCAM Antibody in Flow Cytometry (Flow)
            EpCAM Antibody in Flow Cytometry (Flow)
            EpCAM Antibody in Immunocytochemistry (ICC/IF)
            EpCAM Antibody in Immunocytochemistry (ICC/IF)
            EpCAM Antibody in Immunocytochemistry (ICC/IF)
            EpCAM Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            EpCAM Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            EpCAM Antibody in Immunohistochemistry (IHC)
            EpCAM Antibody in Immunohistochemistry (IHC)
            EpCAM Antibody

            Product Details

            MA5-12436

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            -
            View 2 publications 2 publications

            Immunohistochemistry (IHC)

            -
            View 4 publications 4 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1-2 µg/mL
            View 3 publications 3 publications

            Immunocytochemistry (ICC/IF)

            1:10-1:200
            View 6 publications 6 publications

            Flow Cytometry (Flow)

            0.5-1 µg/test
            View 2 publications 2 publications

            Radioimmune Assays (RIA)

            Assay-dependent
            -

            Affinity Purification (AP)

            -
            View 1 publication 1 publication

            Miscellaneous PubMed (Misc)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Human, Mouse

            Published species

            Human, Mouse, Rat

            Host/Isotype

            Mouse / IgG1, kappa

            Class

            Monoclonal

            Type

            Antibody

            Clone

            323/A3

            Immunogen

            MCF-7 human breast cancer cells
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.2 mg/mL

            Purification

            Protein A/G

            Storage buffer

            PBS, pH 7.4, with 0.05% BSA

            Contains

            0.05% sodium azide

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_10981962

            Product Specific Information

            MA5-12436 targets Epithelial Specific Antigen in FACS, ICC/IF, IHC (P), and RIA applications and shows reactivity with Human and Mouse samples.

            The MA5-12436 immunogen is mCF-7 human breast cancer cells.

            Target Information

            Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Related Products for

            Bioinformatics

            Protein Aliases: Adenocarcinoma-associated antigen; CD326; Cell surface glycoprotein Trop-1; EGP; EGP314; Ep-CAM; Epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; hEGP314; human epithelial glycoprotein-2; KS 1/4 antigen; KSA; lymphocyte antigen 74; Major gastrointestinal tumor-associated protein GA733-2; mEGP314; membrane component, chromosome 4, surface marker (35kD glycoprotein); panepithelial glycoprotein 314; Protein 289A; Trop-1 protein; Tumor-associated calcium signal transducer 1

            View more View less

            Gene Aliases: CD326; DIAR5; EGP; EGP-2; EGP314; EGP40; Ep-CAM; EPCAM; EpCAM1; ESA; GA733-2; gp40; HNPCC8; KS1/4; KSA; Ly74; M1S2; M4S1; MIC18; MK-1; Tacsd1; TACSTD1; TROP1

            View more View less

            UniProt ID: (Human) P16422, (Mouse) Q99JW5

            View more View less

            Entrez Gene ID: (Human) 4072, (Mouse) 17075

            View more View less

            Function(s)

            protein binding protein complex binding cadherin binding involved in cell-cell adhesion

            Process(es)

            ureteric bud development positive regulation of cell proliferation signal transduction involved in regulation of gene expression negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter stem cell differentiation cell-cell adhesion negative regulation of cell-cell adhesion mediated by cadherin positive regulation of cell motility positive regulation of stem cell proliferation
            It has to be done as per old AB suggested Products section.
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