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Invitrogen
WNT1 Monoclonal Antibody (10C8)
Product Details
MA5-15544
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
10C8
Immunogen
Purified recombinant fragment of WNT1 expressed in E. Coli.
Conjugate
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10984682
Product Specific Information
MA5-15544 targets WNT1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human and mouse samples.
The MA5-15544 immunogen is purified recombinant fragment of WNT1 expressed in E. Coli.
MA5-15544 detects WNT1 which has a predicted molecular weight of approximately 41kDa.
Target Information
Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Proto-oncogene Int-1; Proto-oncogene Int-1 homolog; proto-oncogene protein Wnt-1; Proto-oncogene Wnt-1; wingless-related MMTV integration site 1; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1)
Gene Aliases: BMND16; Int-1; INT1; OI15; sw; swaying; Wnt-1; WNT1
UniProt ID: (Human) P04628, (Mouse) P04426
Entrez Gene ID: (Human) 7471, (Mouse) 22408
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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