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            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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            • Primary Antibodies ›
            • IL-1 beta Antibodies

            Invitrogen

            IL-1 beta Monoclonal Antibody (8516), APC

            1 Published Figure
            1 Reference
            View all (114) IL-1 beta antibodies
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            Catalog # MA5-23597
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            Ends: 30-Jun-2025
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            Cite IL-1 beta Monoclonal Antibody (8516), APC

            • Antibody Testing Data (1)
            • Published Figures (1)
            Application
            IL-1 beta Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 2

            IL-1 beta Antibody (MA5-23597) in Flow

            Flow cytometry of IL-1 beta in Peripheral blood mononuclear cell monocytes, resting (open histogram), or LPS-treated (filled histogram). Samples were incubated in IL-1 beta monoclonal antibody (Product # MA5-23597) or isotype control.
            View Product

            IL-1 beta Antibody in Flow Cytometry (Flow)
            IL-1 beta Antibody in Flow Cytometry (Flow)

            Product Details

            MA5-23597

            Applications
            Tested Dilution
            Publications

            Flow Cytometry (Flow)

            10 µL/10^6 cells
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Mouse / IgG1

            Class

            Monoclonal

            Type

            Antibody

            Clone

            8516

            Immunogen

            E. coli-derived recombinant human IL-1 beta/IL-1F2 (aa 117-269)
            View immunogen

            Conjugate

            APC
            • FITC
            • PE 
            • Request custom conjugation

            Excitation/Emission Max

            651/660 nm View spectra spectra

            Form

            Liquid

            Purification

            Protein A/G

            Storage buffer

            Buffered saline solution with 0.5% BSA

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2609798

            Product Specific Information

            This antibody shows less than 5% cross-reactivity with recombinant mouse (rm) IL-1 beta and rpIL-1 beta and no cross-reactivity with rrIL-1 beta, rmIL-1 alpha, rhIL-1ra, rmIL-1ra, or rrIL-1 alpha.

            Target Information

            Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            Bioinformatics

            Protein Aliases: Catabolin; Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; interleukin 1, beta; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); preinterleukin 1 beta; Pro interleukin 1 beta; pro-interleukin-1-beta

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            Gene Aliases: IL-1; IL1-BETA; IL1B; IL1F2

            View more View less

            UniProt ID: (Human) P01584

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            Entrez Gene ID: (Human) 3553

            View more View less

            Function(s)

            cytokine activity interleukin-1 receptor binding protein domain specific binding interleukin superfamily

            Process(es)

            negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPK activity fever generation response to hypoxia positive regulation of protein phosphorylation chronic inflammatory response to antigenic stimulus positive regulation of T cell mediated immunity purine nucleobase metabolic process apoptotic process inflammatory response signal transduction positive regulation of cytosolic calcium ion concentration cell-cell signaling embryo implantation aging memory estrogen metabolic process negative regulation of cell proliferation glycoprotein metabolic process response to heat response to ozone response to gamma radiation positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of glucose transport negative regulation of glutamate secretion smooth muscle adaptation cytokine-mediated signaling pathway pentacyclic triterpenoid metabolic process hyaluronan biosynthetic process neutrophil chemotaxis polyketide metabolic process ovulation sequestering of triglyceride positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of fever generation lipopolysaccharide-mediated signaling pathway positive regulation of prostaglandin secretion response to estradiol interleukin-1 beta production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of immature T cell proliferation in thymus positive regulation of histone phosphorylation response to ATP response to vitamin D response to L-ascorbic acid positive regulation of heterotypic cell-cell adhesion positive regulation of histone acetylation social behavior ectopic germ cell programmed cell death positive regulation of myosin light chain kinase activity response to stilbenoid cellular response to drug response to diuretic response to statin wound healing positive regulation of T cell proliferation positive regulation of NF-kappaB import into nucleus positive regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling response to morphine negative regulation of MAP kinase activity response to peptide hormone protein kinase B signaling positive regulation of JUN kinase activity positive regulation of chemokine biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-6 biosynthetic process positive regulation of nitric oxide biosynthetic process response to ethanol negative regulation of neuron differentiation positive regulation of angiogenesis negative regulation of lipid metabolic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade negative regulation of insulin receptor signaling pathway positive regulation of protein export from nucleus positive regulation of astrocyte differentiation positive regulation of phagocytosis regulation of insulin secretion negative regulation of lipid catabolic process positive regulation of lipid catabolic process regulation of nitric-oxide synthase activity positive regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of cell division positive regulation of cell adhesion molecule production positive regulation of calcidiol 1-monooxygenase activity negative regulation of adiponectin secretion positive regulation of ERK1 and ERK2 cascade monocyte aggregation cellular response to antibiotic cellular response to mechanical stimulus cellular response to organic substance cellular response to glucose stimulus cellular response to fatty acid cellular response to organic cyclic compound cellular response to methotrexate response to dexamethasone positive regulation of monocyte chemotactic protein-1 production positive regulation of neutrophil chemotaxis extrinsic apoptotic signaling pathway in absence of ligand regulation of establishment of endothelial barrier negative regulation of branching morphogenesis of a nerve negative regulation of neural precursor cell proliferation positive regulation of interleukin-6 secretion negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
            It has to be done as per old AB suggested Products section.
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