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Application
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NCF2 Antibody (PA5-80843) in WB
Western Blot using NCF2 Polyclonal Antibody (Product # PA5-80843) at 1:500 dilution. Lane A: THP1 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 60 kDa.
Product Details
PA5-80843
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human NCF2/NCF-2/P67phox protein (Met1-Val526)
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788112
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human NCF2/NCF-2/P67phox.
Target Information
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Defects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2). Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 67 kDa neutrophil oxidase factor; chronic granulomatous disease, autosomal 2; FLJ93058; NADPH oxidase activator 2; NCF-2; Neutrophil cytosol factor 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2); Neutrophil NADPH oxidase factor 2; p67-phox
Gene Aliases: NCF-2; NCF2; NOXA2; P67-PHOX; P67PHOX
UniProt ID: (Human) P19878
Entrez Gene ID: (Human) 4688
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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