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            • Primary Antibodies ›
            • Estrogen Receptor beta Antibodies

            Invitrogen

            Estrogen Receptor beta Polyclonal Antibody

            Advanced Verification
            34 Published Figures
            99 References
            View all (61) Estrogen Receptor beta antibodies
            Datasheet
            Protocols
            Questions & Answers
            Datasheet
            Protocols
            Questions & Answers

            Cite Estrogen Receptor beta Polyclonal Antibody

            • Antibody Testing Data (1)
            • Published Figures (34)
            • Advanced Verification (1)
            Application
            Estrogen Receptor beta Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 36

            Estrogen Receptor beta Antibody (PA1-311) in WB

            Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of LNCap (Lane 1), PC-3 (Lane 2), MCF-7 (Lane 3), MDA-MB-231 (Lane 4), T-47D (Lane 5) and HeLa (Lane 6). The blots were probed with Anti-Rabbit Polyclonal Antibody (Product # PA1-311, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A270... View More
            View Product

            Estrogen Receptor beta Antibody in Western Blot (WB)
            Estrogen Receptor beta Antibody in Western Blot (WB)
            Estrogen Receptor beta Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
            Estrogen Receptor beta Antibody in Immunocytochemistry (ICC/IF)
            Estrogen Receptor beta Antibody in Immunocytochemistry (ICC/IF)
            Estrogen Receptor beta Antibody in Immunocytochemistry (ICC/IF)
            Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor beta Antibody in Western Blot, Immunohistochemistry (WB, IHC)
            Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)
            Estrogen Receptor beta Antibody in Western Blot (WB)
            Estrogen Receptor beta Antibody in Western Blot (WB)
            Estrogen Receptor beta Antibody in Western Blot (WB)
            Estrogen Receptor beta Antibody in Western Blot (WB)
            Estrogen Receptor beta Antibody in Western Blot (WB)
            Estrogen Receptor beta Antibody in Western Blot (WB)
            Estrogen Receptor beta Antibody in Western Blot (WB)
            Estrogen Receptor beta Antibody in ChIP Assay (ChIP)
            Estrogen Receptor beta Antibody in ChIP Assay (ChIP)
            Estrogen Receptor beta Antibody in ChIP Assay (ChIP)
            Estrogen Receptor beta Antibody in ChIP Assay (ChIP)
            Estrogen Receptor beta Antibody in ChIP Assay (ChIP)
            Estrogen Receptor beta Antibody in ChIP Assay (ChIP)
            Estrogen Receptor beta Antibody in ChIP Assay (ChIP)
            Estrogen Receptor beta Antibody

            Product Details

            PA1-311

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1 µg/mL
            View 36 publications 36 publications

            Immunohistochemistry (IHC)

            -
            View 39 publications 39 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1 µg/mL
            View 2 publications 2 publications

            Immunohistochemistry (Frozen) (IHC (F))

            1 µg/mL
            -

            Immunocytochemistry (ICC/IF)

            1 µg/mL
            View 12 publications 12 publications

            Flow Cytometry (Flow)

            -
            View 1 publication 1 publication

            Immunoprecipitation (IP)

            -
            View 1 publication 1 publication

            ChIP assay (ChIP)

            -
            View 6 publications 6 publications

            Gel Shift (GS)

            1 µg/mL
            View 1 publication 1 publication

            Miscellaneous PubMed (Misc)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Human, Mouse, Non-human primate, Sheep, Rat

            Published species

            Bovine, Hamster, Human, Mouse, Non-human primate, Pig, Rat, Sheep

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Synthetic peptide corresponding to residues A(55) E P Q K S P W C E A R S L E H(70) of rat ER beta.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS with 1mg/mL BSA

            Contains

            0.05% sodium azide

            Storage conditions

            -20°C, Avoid Freeze/Thaw Cycles

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_325597

            Product Specific Information

            PA1-311 detects estrogen receptor (ER) beta from human, non-human primate, mouse, ovine and rat tissues.

            PA1-311 has been successfully used in Western blot, immunocytochemistry and gel shift procedures. By Western blot, this antibody detects an ~55-62 kDa protein representing ER beta from rat brain homogenate. PA1-311 efficiently supershifts rat ER beta/ER response element (vitellogenin xenopus A2 gene).

            The PA1-311 immunogen is a synthetic peptide corresponding to residues A(55) E P Q K S P W C E A R S L E H(70) of rat ER beta.

            Recombinant ER beta expressed in baculovirus system (Cat. # RP-311 and RP-312) can be purchased for control experiments in Western blot and gel shift assays.

            PA1-311 can be used with blocking peptide PEP-011.

            Target Information

            Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: ER; ER beta; ER-beta; estrogen receptor 2 (ER beta); estrogen receptor 2 beta; Estrogen receptor beta; estrogen receptor beta 4; Nuclear receptor subfamily 3 group A member 2; oestrogen receptor beta; steroid receptor

            View more View less

            Gene Aliases: ER-BETA; ER[b]; ERB; Erb2; ERBETA; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2

            View more View less

            UniProt ID: (Human) O75584, (Sheep) Q9TU15, (Mouse) O08537, (Rat) Q62986

            View more View less

            Entrez Gene ID: (Human) 2100, (Sheep) 443066, (Mouse) 13983, (Rat) 25149

            View more View less

            Function(s)

            core promoter sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity transcription coactivator activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding steroid binding protein binding drug binding zinc ion binding enzyme binding estrogen receptor activity estrogen response element binding RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding peroxisome proliferator activated receptor binding receptor antagonist activity estradiol binding steroid hormone binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding lipid binding hormone binding sequence-specific DNA binding metal ion binding

            Process(es)

            negative regulation of transcription from RNA polymerase II promoter ovarian follicle development behavioral fear response neuron migration transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter brain development learning or memory negative regulation of cell proliferation hormone-mediated apoptotic signaling pathway intracellular steroid hormone receptor signaling pathway intracellular estrogen receptor signaling pathway response to testosterone regulation of cell proliferation positive regulation of apoptotic process steroid hormone mediated signaling pathway regulation of neuron apoptotic process estrous cycle positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of behavior negative regulation of smooth muscle cell proliferation negative regulation of epithelial cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity Sertoli cell proliferation uterus development vagina development negative regulation of cell death prostate gland epithelium morphogenesis epithelial cell maturation involved in prostate gland development negative regulation of androgen receptor signaling pathway positive regulation of ERK1 and ERK2 cascade negative regulation of neuron death negative regulation of feeding behavior negative regulation of reactive oxygen species metabolic process transcription initiation from RNA polymerase II promoter signal transduction cell-cell signaling response to water deprivation response to activity response to insecticide cerebellum development amygdala development hypothalamus development negative regulation of cell growth prostate gland development response to nutrient levels response to genistein cellular response to drug response to estrogen response to human chorionic gonadotropin response to ethanol positive regulation of vasodilation Sertoli cell development cellular response to magnetism cellular response to estradiol stimulus response to dexamethasone extracellular negative regulation of signal transduction response to salt response to bisphenol A male gonad development female gonad development response to hormone response to organic cyclic compound response to estradiol response to drug cellular response to organic cyclic compound
            It has to be done as per old AB suggested Products section.
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