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Application
FIGURE: 1 / 9
GFP Antibody (PA1-9533) in ICC/IF
Cells 2022 -
Asp-GFP localization during kinetochore-driven microtubule regrowth (KDMTR) after colcemid-induced MT depolymerization. ( A ) S2 cells expressing both Asp-GFP and Cherry-tubulin were fixed and stained with anti-alpha-tubulin (red) and anti-GFP (green) antibodies and counterstained for DNA (DAPI, blue). Panel ( A1 ) shows an early KDMTR stage; panels ( A2 , A3 ) show more advanced KDMTR phases characterized ... View More
Product Details
PA1-9533
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
Purified recombinant Green Fluorescent fusion Protein.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
IgY fraction
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1074893
Product Specific Information
PA1-9533 detects Green Fluorescence Protein in N/A samples.
PA1-9533 has been successfully used in Western blot, ELISA and immunocytochemistry procedures. By Western blot, PA1-9533 detects a 32 kDa protein representing Green Fluorescence Protein.
PA1-9533 immunogen is a purified recombinant green fluorescence protein.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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