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SMG5 Antibody (PA5-119884) in WB
Western blot analysis of SMG5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. SMG5 Polyclonal Antibody (Product # PA5-119884) at 1:1,000 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate. Lane 2: Hela cell lysate. Lane 3: 293 cell lysate. Lane 4: THP-1 cell lysate.
Product Details
PA5-119884
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human SMG5 aa 800-1,000
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913456
Product Specific Information
Positive Control: Jurkat cell lysate, Hela cell lysate, 293 cell lysate, THP-1 cell lysate, mouse testis tissue lysates, rat testis tissue lysates, Hela.
Subcellular Location: Cytoplasm, Nucleus.
Target Information
SMG5 plays a role in nonsense-mediated mRNA decay. SMG5 does not have RNase activity by itself. SMG5 promotes dephosphorylation of RENT1. SMG5 is necessary for TERT activity.SMG5 is involved in nonsense-mediated mRNA decay (Ohnishi et al., 2003 [PubMed 14636577]).[supplied by OMIM].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EST1 telomerase component homolog B; EST1-like protein B; Est1p-like protein B; ever shorter telomeres 1B; FLJ34864; hSMG-5; LPTS interacting protein; LPTS-interacting protein; LPTS-RP1; Nonsense-mediated mRNA decay factor SMG5; protein SMG5; RP11-54H19.7; SMG-5 homolog; Smg-5 homolog, nonsense mediated mRNA decay factor; SMG5 nonsense mediated mRNA decay factor
Gene Aliases: BC024683; EST1B; KIAA1089; LPTS-RP1; LPTSRP1; mKIAA1089; SMG-5; SMG5
UniProt ID: (Human) Q9UPR3, (Mouse) Q6ZPY2
Entrez Gene ID: (Human) 23381, (Mouse) 229512, (Rat) 681012
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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