Invitrogen

ESRP1 Polyclonal Antibody

ESRP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 13

ESRP1 Antibody (PA5-25833) in IHC (P)

Immunohistochemistry analysis of ESRP1 in paraformaldehyde-fixed, paraffin-embedded human pancreas tissue sections. Samples were incubated with ESRP1 polyclonal antibody (Product # PA5-25833) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieva... View More

ESRP1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

ESRP1 Antibody — Figure 5 ESRP1 expression influences CD44v6 expression and is epigenetically downregulated in advanced metastatic melanoma A. Scatter plots showing the correlation analysis of ESRP1 expression and CD44v6/s ratio using TCGA data for PRM. B. Box plot comparing the ESRP1 expression using TCGA RNA-Seq data for PRM, LNM, and DOM specimens. C. Box plots comparing the expression of ESRP1 assessed by qPCR for PRM, LNM, non-MBM, and MBM. D. Box plot comparing ESRP1 expression for PRM with and without MBM-development. E. Western blot showing ESRP1 and CD44v6 expression on two LNM cell lines (MB and LH) treated with siRNA control (siCTRL) and siRNA ESRP1 (siESRP1). F. Box plot comparing the DNA methylation level of ESRP1 gene in PRM, LNM, non-MBM, and MBM specimens. * is p = 0.05 and ** is p = 0.01. {KD}

Product Details

PA5-25833

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 182-211 amino acids of human ESRP1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2543333

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

RBM35A, also known as ESRP1, is a mRNA splicing factor that with its related protein RBM35B (ESRP2) are coordinators of an epithelial cell-type-specific splicing program. RBM35A contains three putative RNA recognition motifs and acts by directly binding specific sequences in mRNAs. RBM35A is involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs. Other recent studies have shown that RMB35A may also act as a novel tumor suppressor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tropomyosin1 isoforms underlie epithelial to mesenchymal plasticity, metastatic dissemination, and resistance to chemotherapy in high-grade serous ovarian cancer.

Cell death and differentiation

Xu T,Verhagen MP,Teeuwssen M,Sun W,Joosten R,Sacchetti A,Ewing-Graham PC,Jansen MPHM,Boere IA,Bryce NS,Zeng J,Treutlein HR,Hook J,Hardeman EC,Gunning PW,Fodde R

Published figure using ESRP1 polyclonal antibody (Product # PA5-25833) in Western Blot

Fri Mar 01 00:00:00 UTC 2024

ESRP1 controls biogenesis and function of a large abundant multiexon circRNA.

Nucleic acids research

Liu D,Dredge BK,Bert AG,Pillman KA,Toubia J,Guo W,Dyakov BJA,Migault MM,Conn VM,Conn SJ,Gregory PA,Gingras AC,Patel D,Wu B,Goodall GJ

Published figure using ESRP1 polyclonal antibody (Product # PA5-25833) in Western Blot

Fri Feb 09 00:00:00 UTC 2024

The calcium channel TRPC6 promotes chemotherapy-induced persistence by regulating integrin α6 mRNA splicing.

Cell reports

Mukhopadhyay D,Goel HL,Xiong C,Goel S,Kumar A,Li R,Zhu LJ,Clark JL,Brehm MA,Mercurio AM

Published figure using ESRP1 polyclonal antibody (Product # PA5-25833) in Western Blot

Tue Nov 28 00:00:00 UTC 2023

CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms.

Nucleic acids research

Batsché E,Yi J,Mauger O,Kornobis E,Hopkins B,Hanmer-Lloyd C,Muchardt C

PA5-25833 was used in Western Blot to explore the possibility of the impact of DNA methylation on alternative splicing that favors tumor progression.

Mon Jun 21 00:00:00 UTC 2021

Protocol for proteogenomic dissection of intronic splicing enhancer interactome for prediction of individualized cancer prognosis.

STAR protocols

Wang L,Wrobel JA,Xie L,Chen X

PA5-25833 was used in Western Blot to demonstrate a protocol for an alternative splicing activity-based proteogenomic approach for identification of candidate prognostic markers in cancer cell lines and human breast cancer specimens.

Fri Mar 19 00:00:00 UTC 2021

Brain metastasis is predetermined in early stages of cutaneous melanoma by CD44v6 expression through epigenetic regulation of the spliceosome.

Pigment cell & melanoma research

Marzese DM,Liu M,Huynh JL,Hirose H,Donovan NC,Huynh KT,Kiyohara E,Chong K,Cheng D,Tanaka R,Wang J,Morton DL,Barkhoudarian G,Kelly DF,Hoon DS

PA5-25833 was used in immunohistochemistry and western blot to examine CD44 variants during the progression of melanoma to melanoma brain metastasis.

Thu Jan 01 00:00:00 UTC 2015

Brain metastasis is predetermined in early stages of cutaneous melanoma by CD44v6 expression through epigenetic regulation of the spliceosome.

Pigment cell & melanoma research

Marzese DM,Liu M,Huynh JL,Hirose H,Donovan NC,Huynh KT,Kiyohara E,Chong K,Cheng D,Tanaka R,Wang J,Morton DL,Barkhoudarian G,Kelly DF,Hoon DS

PA5-25833 was used in immunohistochemistry and western blot to examine CD44 variants during the progression of melanoma to melanoma brain metastasis.

Thu Jan 01 00:00:00 UTC 2015

Brain metastasis is predetermined in early stages of cutaneous melanoma by CD44v6 expression through epigenetic regulation of the spliceosome.

Pigment cell & melanoma research

Marzese DM,Liu M,Huynh JL,Hirose H,Donovan NC,Huynh KT,Kiyohara E,Chong K,Cheng D,Tanaka R,Wang J,Morton DL,Barkhoudarian G,Kelly DF,Hoon DS

PA5-25833 was used in immunohistochemistry and western blot to examine CD44 variants during the progression of melanoma to melanoma brain metastasis.

Thu Jan 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Epithelial splicing regulatory protein 1; RNA binding motif protein 35A; RNA-binding motif protein 35A; RNA-binding protein 35A

View more View less

Gene Aliases: ESRP1; RBM35A; RMB35A

View more View less

UniProt ID: (Human) Q6NXG1

View more View less

Entrez Gene ID: (Human) 54845

View more View less

Function(s)

nucleotide binding mRNA binding protein binding RNA splicing factor

Process(es)

mRNA processing RNA splicing fibroblast growth factor receptor signaling pathway regulation of RNA splicing

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ESRP1 Polyclonal Antibody

ESRP1 Antibody (PA5-25833) in IHC (P)

Product Details

PA5-25833

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!