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Reelin Antibody (PA5-78413) in ICC/IF
Reelin Polyclonal Antibody detects Reelin protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Reelin protein stained by Reelin Polyclonal Antibody (Product # PA5-78413) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta-3 Tubulin Polyclonal Antibody [GT1338]diluted at 1:500. Blue: Fluoroshield with DAPI .
Product Details
PA5-78413
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of mouse Reelin. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.19 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2736514
Product Specific Information
Positive Control: mouse brain, rat brain
Predicted Reactivity: Human (97%), Chicken (91%), Sheep (98%), Bovine (97%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Reelin in an extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: extracellular matrix serine protease; Reeler protein; Reelin
Gene Aliases: Reelen; reeler; Reln; Rl
UniProt ID: (Mouse) Q60841, (Rat) P58751
Entrez Gene ID: (Mouse) 19699, (Rat) 24718
protein binding
peptidase activity
serine-type peptidase activity
hydrolase activity
metal ion binding
lipoprotein particle receptor binding
very-low-density lipoprotein particle receptor binding
molecular_function
protein serine/threonine/tyrosine kinase activity
cell adhesion molecule
extracellular matrix glycoprotein
extracellular matrix protein
cell morphogenesis involved in differentiation
neuron migration
proteolysis
cell adhesion
multicellular organism development
axon guidance
central nervous system development
brain development
long-term memory
locomotory behavior
associative learning
glial cell differentiation
regulation of gene expression
positive regulation of neuron projection development
positive regulation of phosphatidylinositol 3-kinase signaling
dendrite development
cell migration
peptidyl-tyrosine phosphorylation
spinal cord patterning
dentate gyrus development
hippocampus development
cerebral cortex tangential migration
layer formation in cerebral cortex
cerebral cortex development
forebrain development
positive regulation of TOR signaling
positive regulation of CREB transcription factor activity
protein localization to synapse
reelin-mediated signaling pathway
thyroid hormone metabolic process
positive regulation of protein kinase activity
response to pain
positive regulation of peptidyl-tyrosine phosphorylation
regulation of behavior
modulation of synaptic transmission
positive regulation of small GTPase mediated signal transduction
positive regulation of synaptic transmission, glutamatergic
positive regulation of dendritic spine morphogenesis
positive regulation of protein tyrosine kinase activity
positive regulation of synapse maturation
postsynaptic density assembly
NMDA glutamate receptor clustering
postsynaptic density protein 95 clustering
receptor localization to synapse
lateral motor column neuron migration
positive regulation of long-term synaptic potentiation
positive regulation of lateral motor column neuron migration
regulation of N-methyl-D-aspartate selective glutamate receptor activity
positive regulation of excitatory postsynaptic potential
positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
midgut development
memory
response to mechanical stimulus
response to toxic substance
response to hormone
response to lithium ion
response to organic cyclic compound
response to insecticide
ventral spinal cord development
orbitofrontal cortex development
hypothalamus development
response to progesterone
social behavior
response to drug
response to amino acid
response to ethanol
response to axon injury
response to corticosterone
response to electrical stimulus
response to fatty acid
cellular response to dexamethasone stimulus
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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