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ATF1 Antibody (PA5-78829) in Flow
Flow Cytometry of ATF1 in HeLa cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum and then incubated with ATF1 Polyclonal Antibody, DyLight™ 488 (Product # PA5-78829) at a dilution of 1 μg (per 1x10^6 cells) for 30 min at 20°C.
Product Details
PA5-78829
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human ATF1 recombinant protein (Position: M1-V271).
Conjugate
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
200 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745945
Target Information
This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activating transcription factor 1; cAMP-dependent transcription factor ATF-1; Cyclic AMP-dependent transcription factor ATF-1; Protein TREB36
Gene Aliases: ATF1; EWS-ATF1; FUS/ATF-1; TREB36
UniProt ID: (Human) P18846
Entrez Gene ID: (Human) 466
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
protein complex binding
protein heterodimerization activity
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