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Application
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GAD65 Antibody (PA5-79294) in IHC (P)
Immunohistochemistry analysis of GAD65 on paraffin-embedded human mammary cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with GAD65 polyclonal antibody (Product# PA5-79294) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C).... View More
Product Details
PA5-79294
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human GAD65 recombinant protein (Position: K84-L182).
Conjugate
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746410
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, mouse brain tissue. IHC: human glioma tissue, human mammary cancer tissue.
Target Information
GAD65 (glutamic acid decarboxylase-65) are present in autoimmune disorders such as insulin-dependent (type1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 2; Glutamate decarboxylase 2 (islet); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase-2 (pancreas); glutamic acid decarboxylase 2; glutamic acid decarboxylase 65; MGC161605; MGC161607; RP11-420F12.2
Gene Aliases: 6330404F12Rik; GAD(65); Gad-2; GAD2; GAD65
UniProt ID: (Human) Q05329, (Mouse) P48320, (Rat) Q05683
Entrez Gene ID: (Human) 2572, (Mouse) 14417, (Rat) 24380
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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