Invitrogen

SOX2 Monoclonal Antibody (20G5)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

10 Published Figures

28 References

View all (90) SOX2 antibodies

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Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

SOX2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 28

SOX2 Antibody (MA1-014) in ICC/IF

Immunofluorescent analysis of Sox2 (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Sox2 monoclonal antibody (Product # MA1-014) at a dilution of 1:200 overnight at 4°C, washed with PBST, and incubated with a FITC-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fl... View More

SOX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SOX2 Antibody in Immunoprecipitation (IP)

SOX2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

SOX2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

SOX2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell line tested owing to their inherent genetic constitution. Relative expression of SOX2 was observed in NTERA2 and NCCIT in comparision to HeLa using Anti-SOX2 Monoclonal Antibody (20G5) (Product # MA1-014) in Western Blot. {RE}

Product Details

MA1-014

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:200

View 9 publications 9 publications

Flow Cytometry (Flow)

1:100

Immunoprecipitation (IP)

5 µg

ChIP assay (ChIP)

1-3 µL

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Fish, Human, Mouse

Published species

Bovine, Dog, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

20G5

Immunogen

Full-length human recombinant protein expressed in bacteria

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 30% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536667

Product Specific Information

Western blot analysis of MA1-014 specifically detects SOX2 protein at ~36 kDa in the nucleus of human and mouse embryonal carcinoma and iPS cells.

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deletion of TRPC6, an Autism Risk Gene, Induces Hyperexcitability in Cortical Neurons Derived from Human Pluripotent Stem Cells.

Molecular neurobiology

Shin KC,Ali G,Ali Moussa HY,Gupta V,de la Fuente A,Kim HG,Stanton LW,Park Y

MA1-014 was used in Immunohistochemistry-immunofluorescence to provide evidence that reduction of SOCE by TRPC6 KO results in neuronal hyperexcitability, which we hypothesize is an important contributor to the cellular pathophysiology underlying hyperactivity in some ASD.

Fri Dec 01 00:00:00 EST 2023

The Generation of Human iPSC Lines from Three Individuals with Dravet Syndrome and Characterization of Neural Differentiation Markers in iPSC-Derived Ventral Forebrain Organoid Model.

Cells

Zayat V,Kuczynska Z,Liput M,Metin E,Rzonca-Niewczas S,Smyk M,Mazurczak T,Goszczanska-Ciuchta A,Leszczynski P,Hoffman-Zacharska D,Buzanska L

MA1-014 was used in Immunocytochemistry-immunofluorescence to generate iPSC lines from fibroblasts obtained from three individuals affected with DRVT carrying distinct mutations in the SCN1A gene (nonsense mutation p.Ser1516*, missense mutation p.Arg1596His, and splicing mutation c.2589+2dupT).

Mon Jan 16 00:00:00 EST 2023

Survivin Inhibition by Piperine Sensitizes Glioblastoma Cancer Stem Cells and Leads to Better Drug Response.

International journal of molecular sciences

Warrier NM,Krishnan RK,Prabhu V,Hariharapura RC,Agarwal P,Kumar P

Published figure using SOX2 monoclonal antibody (Product # MA1-014) in Immunocytochemistry

Sat Jul 09 00:00:00 EDT 2022

Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos.

International journal of molecular sciences

Ordóñez-León EA,Martínez-Rodero I,García-Martínez T,López-Béjar M,Yeste M,Mercade E,Mogas T

MA1-014 was used in Immunohistochemistry to suggest that 100 µg/mL EPS ID1 added to the vitrification media is beneficial for embryo cryopreservation because it results in higher re-expansion and hatching ability and it positively modulates apoptosis.

Sat Jun 25 00:00:00 EDT 2022

Dentate gyrus morphogenesis is regulated by β-catenin function in hem-derived fimbrial glia.

Development (Cambridge, England)

Parichha A,Datta D,Suresh V,Chatterjee M,Holtzman MJ,Tole S

MA1-014 was used in Immunohistochemistry-immunofluorescence to highlight a primary requirement for β-catenin for the organization of the fimbrial scaffold and a secondary role for this factor in dentate gyrus morphogenesis.

Tue Nov 01 00:00:00 EDT 2022

Stem cell phenotype predicts therapeutic response in glioblastomas with MGMT promoter methylation.

Acta neuropathologica communications

Lakis NS,Brodsky AS,Karashchuk G,Audesse AJ,Yang D,Sturtevant A,Lombardo K,Wong IY,Webb AE,Anthony DC

MA1-014 was used in Immunohistochemistry to support the concept that the presence of a high stem cell phenotype in GBM, as marked by expression of SOX2 or CD133, may be associated with the clinical response to treatment.

Fri Nov 04 00:00:00 EDT 2022

Impact of equilibration duration combined with temperature on the outcome of bovine oocyte vitrification.

Theriogenology

García-Martínez T,Martínez-Rodero I,Roncero-Carol J,Yánez-Ortiz I,Higgins AZ,Mogas T

MA1-014 was used in Immunohistochemistry to reveal that the limitation of the exposure time of the oocytes to the equilibration solution (ES) to the point of osmotic equilibrium volume recovery could be a more efficient approach to prepare them for vitrification.

Sun May 01 00:00:00 EDT 2022

Novel fragile X syndrome 2D and 3D brain models based on human isogenic FMRP-KO iPSCs.

Cell death & disease

Brighi C,Salaris F,Soloperto A,Cordella F,Ghirga S,de Turris V,Rosito M,Porceddu PF,D'Antoni C,Reggiani A,Rosa A,Di Angelantonio S

MA1-014 was used in Immunohistochemistry to demonstrate that FMRP is required to correctly support neuronal and glial cell proliferation, and to set the correct excitation/inhibition ratio in human brain development.

Sat May 15 00:00:00 EDT 2021

Novel fragile X syndrome 2D and 3D brain models based on human isogenic FMRP-KO iPSCs.

Cell death & disease

Brighi C,Salaris F,Soloperto A,Cordella F,Ghirga S,de Turris V,Rosito M,Porceddu PF,D'Antoni C,Reggiani A,Rosa A,Di Angelantonio S

Sat May 15 00:00:00 EDT 2021

Glycoproteomic analysis of the changes in protein N-glycosylation during neuronal differentiation in human-induced pluripotent stem cells and derived neuronal cells.

Scientific reports

Kimura K,Koizumi T,Urasawa T,Ohta Y,Takakura D,Kawasaki N

MA1-014 was used in Western Blot to conduct sequential LC/MS/MS analyses of tryptic digest, enriched glycopeptides, and deglycosylated peptides of proteins derived from human-induced pluripotent stem cells (iPSCs) and iPSC-derived neuronal cells.

Thu May 27 00:00:00 EDT 2021

Glycoproteomic analysis of the changes in protein N-glycosylation during neuronal differentiation in human-induced pluripotent stem cells and derived neuronal cells.

Scientific reports

Kimura K,Koizumi T,Urasawa T,Ohta Y,Takakura D,Kawasaki N

Thu May 27 00:00:00 EDT 2021

Guided Self-Assembly of ES-Derived Lung Progenitors into Biomimetic Tube Structures That Impact Cell Differentiation.

Bioengineering (Basel, Switzerland)

Soleas JP,Huang L,D'Arcangelo E,Nostro MC,Waddell TK,McGuigan AP,Karoubi G

MA1-014 was used in Immunocytochemistry to suggest that geometric cues impose constraints on cells, homogenize cellular morphology, and influence fate status.

Fri Dec 10 00:00:00 EST 2021

Deconvolution of cell type-specific drug responses in human tumor tissue with single-cell RNA-seq.

Genome medicine

Zhao W,Dovas A,Spinazzi EF,Levitin HM,Banu MA,Upadhyayula P,Sudhakar T,Marie T,Otten ML,Sisti MB,Bruce JN,Canoll P,Sims PA

MA1-014 was used in Immunohistochemistry to anticipate that this approach will facilitate pre-clinical studies that identify effective therapies for solid tumors.

Tue May 11 00:00:00 EDT 2021

Establishment of a New Cell Line of Canine Mammary Tumor CMT-1026.

Frontiers in veterinary science

Mei C,Xin L,Liu Y,Lin J,Xian H,Zhang X,Hu W,Xia Z,Wang H,Lyu Y

MA1-014 was used in Immunocytochemistry to describe a newly established canine cell line, CMT-1026.

Sat Oct 30 00:00:00 EDT 2021

Neddylation is critical to cortical development by regulating Wnt/β-catenin signaling.

Proceedings of the National Academy of Sciences of the United States of America

Zhang L,Jing H,Li H,Chen W,Luo B,Zhang H,Dong Z,Li L,Su H,Xiong WC,Mei L

MA1-014 was used in Immunohistochemistry to provide evidence for an indispensable role of neddylation, a ubiquitylation-like protein modification, in inhibiting Wnt/β-catenin signaling.

Tue Oct 20 00:00:00 EDT 2020

EGF and a GSK3 Inhibitor Deplete Junctional E-cadherin and Stimulate Proliferation in the Mature Mammalian Ear.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kozlowski MM,Rudolf MA,Corwin JT

MA1-014 was used in Immunohistochemistry to report the discovery of a pharmacological treatment that thins F-actin bands, depletes E-cadherin, and stimulates proliferation in long-quiescent supporting cells within a balance epithelium from adult mice.

Wed Mar 25 00:00:00 EDT 2020

Cancer Stem-Like Cells in a Case of an Inflammatory Myofibroblastic Tumor of the Lung.

Frontiers in oncology

Masciale V,Grisendi G,Banchelli F,D'Amico R,Maiorana A,Sighinolfi P,Brugioni L,Stefani A,Morandi U,Dominici M,Aramini B

Published figure using SOX2 monoclonal antibody (Product # MA1-014) in Immunohistochemistry

Mon Sep 28 00:00:00 EDT 2020

Scaffold-based 3D cellular models mimicking the heterogeneity of osteosarcoma stem cell niche.

Scientific reports

Bassi G,Panseri S,Dozio SM,Sandri M,Campodoni E,Dapporto M,Sprio S,Tampieri A,Montesi M

MA1-014 was used in Immunocytochemistry-immunofluorescence to highlight the fundamental role of the tumour microenvironment proving the mimicry of osteosarcoma stem cell niche by the use of CSCs together with the biomimetic scaffolds, compared to conventional 2D culture systems.

Fri Dec 18 00:00:00 EST 2020

Scaffold-based 3D cellular models mimicking the heterogeneity of osteosarcoma stem cell niche.

Scientific reports

Bassi G,Panseri S,Dozio SM,Sandri M,Campodoni E,Dapporto M,Sprio S,Tampieri A,Montesi M

Fri Dec 18 00:00:00 EST 2020

Expression of ALDH and SOX-2 in Pulmonary Sclerosing Pnemocytoma (PSP) of the Lung: Is There a Meaning Behind?

Frontiers in medicine

Aramini B,Masciale V,Manfredini B,Bianchi D,Banchelli F,D'Amico R,Bertolini F,Dominici M,Morandi U,Maiorana A

MA1-014 was used in Immunohistochemistry-immunofluorescence to highlight the elevated expression of ALDH and the presence of SOX-2 in pulmonary sclerosing pneumocytoma.

Thu Oct 01 00:00:00 EDT 2020

Expression of ALDH and SOX-2 in Pulmonary Sclerosing Pnemocytoma (PSP) of the Lung: Is There a Meaning Behind?

Frontiers in medicine

Aramini B,Masciale V,Manfredini B,Bianchi D,Banchelli F,D'Amico R,Bertolini F,Dominici M,Morandi U,Maiorana A

MA1-014 was used in Immunohistochemistry-immunofluorescence to highlight the elevated expression of ALDH and the presence of SOX-2 in pulmonary sclerosing pneumocytoma.

Thu Oct 01 00:00:00 EDT 2020

Generation of Complete Multi-Cell Type Lung Organoids From Human Embryonic and Patient-Specific Induced Pluripotent Stem Cells for Infectious Disease Modeling and Therapeutics Validation.

Current protocols in stem cell biology

Leibel SL,McVicar RN,Winquist AM,Niles WD,Snyder EY

MA1-014 was used in Immunocytochemistry-immunoflourescence to describe a system that allows human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) to differentiate and form three-dimensional (3D) structures that emulate the development, cytoarchitecture, and function of the lung ("organoids"), containing epithelial and mesenchymal cell populations, and including the production of surfactant and presence of ciliated cells.

Tue Sep 01 00:00:00 EDT 2020

Dysfunction of iPSC-derived endothelial cells in human Hutchinson-Gilford progeria syndrome.

Cell cycle (Georgetown, Tex.)

Matrone G,Thandavarayan RA,Walther BK,Meng S,Mojiri A,Cooke JP

MA1-014 was used in Western Blotting to investigate the dysfunction and differences in morphology of iPSC-derived endothelial cells extracted from HGPS patients compared to unaffected relatives.

Tue Oct 01 00:00:00 EDT 2019

Cancer stem-neuroendocrine cells in an atypical carcinoid case report.

Translational lung cancer research

Masciale V,Grisendi G,Banchelli F,D'Amico R,Maiorana A,Morandi U,Dominici M,Aramini B

Published figure using SOX2 monoclonal antibody (Product # MA1-014) in Immunohistochemistry

Sun Dec 01 00:00:00 EST 2019

MELK inhibition targets cancer stem cells through downregulation of SOX2 expression in head and neck cancer cells.

Oncology reports

Ren L,Deng B,Saloura V,Park JH,Nakamura Y

MA1-014 was used in Western Blotting to investigate if maternal embryonic leucine zipper kinase serves a key role in cancer stem cells through the regulation of SRY-box 2 and maternal embryonic leucine zipper kinase inhibition may also be promising for clinical applications in the treatment of HNSCC.

Mon Apr 01 00:00:00 EDT 2019

GPD1 Specifically Marks Dormant Glioma Stem Cells with a Distinct Metabolic Profile.

Cell stem cell

Rusu P,Shao C,Neuerburg A,Acikgöz AA,Wu Y,Zou P,Phapale P,Shankar TS,Döring K,Dettling S,Körkel-Qu H,Bekki G,Costa B,Guo T,Friesen O,Schlotter M,Heikenwalder M,Tschaharganeh DF,Bukau B,Kramer G,Angel P,Herold-Mende C,Radlwimmer B,Liu HK

MA1-014 was used in Immunohistochemistry to investigate an attractive therapeutic target for treating brain tumors.

Thu Aug 01 00:00:00 EDT 2019

Generation of two human isogenic iPSC lines from fetal dermal fibroblasts.

Stem cell research

Tandon R,Brändl B,Baryshnikova N,Landshammer A,Steenpaß L,Keminer O,Pless O,Müller FJ

MA1-014 was used in Immunocytochemistry-immunoflourescence to show that both new cell lines offer the opportunity to study basic stem cell biology and model human disease.

Sat Dec 01 00:00:00 EST 2018

Modeling Short QT Syndrome Using Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Journal of the American Heart Association

El-Battrawy I,Lan H,Cyganek L,Zhao Z,Li X,Buljubasic F,Lang S,Yücel G,Sattler K,Zimmermann WH,Utikal J,Wieland T,Ravens U,Borggrefe M,Zhou XB,Akin I

MA1-014 was used in Immunocytochemistry to establish a cellular model of short QT syndrome, using human-induced pluripotent stem cell-derived cardiomyocytes.

Sat Mar 24 00:00:00 EDT 2018

Conservative site-specific and single-copy transgenesis in human LINE-1 elements.

Nucleic acids research

Vijaya Chandra SH,Makhija H,Peter S,Myint Wai CM,Li J,Zhu J,Ren Z,D'Alcontres MS,Siau JW,Chee S,Ghadessy FJ,Dröge P

MA1-014 was used in western blot to discuss Phage lambda integrase as a transgenesis tool

Thu Apr 07 00:00:00 EDT 2016

Disentangling the aneuploidy and senescence paradoxes: a study of triploid breast cancers non-responsive to neoadjuvant therapy.

Histochemistry and cell biology

Gerashchenko BI,Salmina K,Eglitis J,Huna A,Grjunberga V,Erenpreisa J

MA1-014 was used in immunohistochemistry to analyze triploid breast cancers non-responsive to neoadjuvant therapy by study of aneuploidy and senescence paradoxes

Fri Apr 01 00:00:00 EDT 2016

Role of stress-activated OCT4A in the cell fate decisions of embryonal carcinoma cells treated with etoposide.

Cell cycle (Georgetown, Tex.)

Huna A,Salmina K,Erenpreisa J,Vazquez-Martin A,Krigerts J,Inashkina I,Gerashchenko BI,Townsend PA,Cragg MS,Jackson TR

Published figure using SOX2 monoclonal antibody (Product # MA1-014) in Western Blot

Wed Aug 03 00:00:00 EDT 2016

Hopx distinguishes hippocampal from lateral ventricle neural stem cells.

Stem cell research

Li D,Takeda N,Jain R,Manderfield LJ,Liu F,Li L,Anderson SA,Epstein JA

Published figure using SOX2 monoclonal antibody (Product # MA1-014) in Immunofluorescence

Sun Nov 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2

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Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb

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UniProt ID: (Human) P48431, (Mouse) P48432

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Entrez Gene ID: (Human) 6657, (Dog) 488092, (Mouse) 20674

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Function(s)

transcription regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding miRNA binding sequence-specific DNA binding transcription regulatory region DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding chromatin binding transcription factor binding chromatin DNA binding protein heterodimerization activity HMG box transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation eye development endodermal cell fate specification chromatin organization regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell cycle arrest response to wounding regulation of gene expression glial cell fate commitment pituitary gland development adenohypophysis development positive regulation of cell-cell adhesion forebrain development somatic stem cell population maintenance tissue regeneration regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade positive regulation of cell differentiation negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter inner ear development negative regulation of epithelial cell proliferation response to growth factor negative regulation of canonical Wnt signaling pathway neuronal stem cell population maintenance cell fate specification positive regulation of neuroblast proliferation transcription, DNA-templated regulation of transcription from RNA polymerase II promoter multicellular organism development sensory perception of sound response to organic substance stem cell population maintenance forebrain neuron differentiation cerebral cortex development negative regulation of Wnt signaling pathway male genitalia development positive regulation of epithelial cell differentiation olfactory placode formation response to retinoic acid inner ear morphogenesis tongue development cell fate commitment negative regulation of cell differentiation positive regulation of neuron differentiation negative regulation of osteoblast differentiation positive regulation of Notch signaling pathway pigment biosynthetic process lung alveolus development embryonic organ development anatomical structure formation involved in morphogenesis neuron fate commitment diencephalon morphogenesis stem cell differentiation regulation of neurogenesis detection of mechanical stimulus involved in sensory perception of sound detection of mechanical stimulus involved in equilibrioception retina morphogenesis in camera-type eye lens induction in camera-type eye epithelial tube branching involved in lung morphogenesis

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SOX2 Monoclonal Antibody (20G5)

SOX2 Antibody (MA1-014) in ICC/IF

Product Details

MA1-014

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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