Search Thermo Fisher Scientific
 |  |  |  |  |  |  |
| 2 | 405 | 450/50 | 404 | 455 | (in buffer) 1 | flow cytometry |
Invitrogen Pacific Blue is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser, with excitation/emission at 404/455 nm. It is optimized for use in flow cytometry applications but can also be used for high-abundance targets in fluorescence microscopy. Conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm respectively, enabling two-color analysis.
We offer Pacific Blue dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. Additionally, reactive dyes and protein labeling kits are available to help you generate your own antibody conjugates or probes.
Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.) Search Pacific Blue primary antibodies
Additional products
Multicolor analysis of CD4-positive and CD8-positive populations using Qdot 605 streptavidin and a Pacific Blue dye-labeled conjugate. Human mononuclear cells (PBMCs) were blocked with nonspecific goat IgG and then stained with mouse anti-human CD4 Monoclonal Antibody (RPA-T4), Biotin. Cells were incubated with Qdot 605 Streptavidin Conjugate, then with mouse anti-human CD8 Monoclonal Antibody (3B5), Pacific Blue. Cells were analyzed on a flow cytometer equipped with a 405 nm violet diode laser and 450/50 and 605/20 nm bandpass filters. Compensation was performed using single-color controls. Cells were analyzed using a lymphocyte gate as determined by FSC/SSC. These reagents give clear separation of the CD4-positive and CD8-positive populations using a single excitation source, the violet diode laser.
Staining of human PBLs with Pacific Blue conjugated antibody. Human peripheral blood lymphocytes (PBLs) were stained using CD3 Monoclonal Antibody (S4.1), Pacific Blue. The negative control profiles represent the unstained cells.
Violet laser–compatible antibody conjugates
We offer Pacific Blue and Pacific Orange dyes, as well as Qdot probes, for use with the violet laser. In addition to primary and secondary antibody conjugates of these dyes, we have developed kits for cell proliferation, immunophenotyping, dead cell discrimination, apoptosis, and cell cycle analysis—all aimed at helping you maximize the data you can get from your violet laser-equipped flow cytometer.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.