1x1 image pixel for data collection
SuperBlock™ Blocking Buffer
SuperBlock™ Blocking Buffer
Thermo Scientific™

SuperBlock™ Blocking Buffer

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.
Promo ImagePromotion
Promo code:RBLOT25
Save up to 24% on Western Blot Reagents and Gels
From now until December 31st, get 16% off when you spend $400 or more, and get 24% off when you spend $750 or more on select western blot reagent and protein gels.Learn More
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantityProduct TypeBuffer
37536Promo Image1 LBlocking BufferTBST
37580Promo Image100 mLBlocking BufferPBS
37515Promo Image1 LBlocking BufferPBS
375185 LBlocking BufferPBS
37581Promo Image100 mLBlocking BufferTBS
37535Promo Image1 LBlocking BufferTBS
37545Promo Image5 packsBlocking Buffer, dry blendTBS
37516Promo Image1 LBlocking BufferPBST
Catalog number 37536
Price (USD)
264.65
Online Exclusive
293.00
Save 28.35 (10%)
Each
In stock
Add to cart
Quantity:
1 L
Product Type:
Blocking Buffer
Buffer:
TBST
Request bulk or custom format
Price (USD)
264.65
Online Exclusive
293.00
Save 28.35 (10%)
Each
Add to cart
SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis. Blocking micro-plates, membranes, or tissues with a SuperBlock buffer yields a high signal-to-noise ratio in most detection systems.
Features of SuperBlock Blocking Buffer include:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes, or tissues with SuperBlock buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Blocking AgentSingle purified protein
BufferTBST
Concentration1X
For Use With (Application)ELISA, Western Blot
FormLiquid
Product LineSuperBlock™
Product TypeBlocking Buffer
Quantity1 L
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C. Product is shipped at ambient temperature.

Frequently asked questions (FAQs)

What should I do about the colloidal carbon (black precipitate) in SuperBlock Blocking Buffer solution?

Allow any colloidal carbon, if present, to settle to the bottom of the bottle and pouring off what you need to use. We do not recommend centrifugation or filtering because these procedures may reduce the product's blocking capabilities.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What preservative is used in SuperBlock Blocking Buffer, and what is its source?

Kathon Preservative, a broad spectrum antimicrobial agent, is used at a concentration of 600 ppm.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is SuperBlock Blocking Buffer's shelf life?

Its shelf life is one year at 4°C.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What incubation time and temperature should I use for effective blocking using the SuperBlock Blocking Buffer ?

Typical procedures use 1 hour at room temperature or overnight at 4°C. However, sufficient blocking in many procedures is possible in 3 x 2 minutes at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is SuperBlock Blocking Buffer RNase-free?

No, SuperBlock Blocking Buffer is not RNase-free.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Documents & Downloads

Certificates

Safety Data Sheets

Share catalog number, name or link