The Ambion™ TaqMan™ Cells-to-C_T Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-C_T Kit. The Control Kit includes XenoRNA™ Control, a synthetic RNA transcript with a unique sequence that lacks homology to current annotated biological sequences, making it an ideal control for any experiment. The Control Kit also includes a TaqMan™ Gene Expression Assay for the XenoRNA™ Control target, and a TaqMan™ Gene Expression Assay for the highly expressed endogenous control gene β-actin.

Features of the The Ambion™ TaqMan™ Cells-to-C_T Control Kit:
• Confirms sufficient cell input
• Monitors the presence of RT or real-time PCR inhibitors
• Controls for sample normalization

Monitor Inhibition and Confirm Sufficient Cell Input
The TaqMan™ Cells-to-C_T™ Control Kit is used to assess inhibition and cell input in Cells-to-C_T reactions. For example, the XenoRNA™ Control confirms that samples can support RT-PCR and is an indicator of reaction inhibitors. By adding the XenoRNA™ Control to the Stop Solution during the last step of the cell lysis procedure, each sample is provided with a constant amount of control target and subsequently amplified by the XenoRNA™ TaqMan™ Gene Expression Assay.

Amplification with the β-actin Gene Expression Assay functions as an endogenous control for normalization, and ensures that the lysis reaction contains a sufficient number of cells—this is important for experiments with fewer than ∼100 cells and monitors the effectiveness of cell lysis.

Evaluate Compatibility with New Cell Lines
The TaqMan™ Cells-to-C_T™ Control Kit is ideal for assessing the compatibility of untested cell lines with the TaqMan™ Gene Expression Cells-to-C_T Kit to determine the maximum number of cells that can be used per lysis reaction.