The ELISA Buffer Kit contains all the basic buffers necessary for use of Invitrogen Antibody Pairs or that are requiredRead more
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Catalog Number
Quantity
CNB0011
10 plates
Catalog number CNB0011
Price (USD)
589.65
Online Exclusive
624.00
Save 34.35 (6%)
Each
Add to cart
Quantity:
10 plates
Price (USD)
589.65
Online Exclusive
624.00
Save 34.35 (6%)
Each
Add to cart
The ELISA Buffer Kit contains all the basic buffers necessary for use of Invitrogen Antibody Pairs or that are required to run an ELISA. The kit provides sufficient material for 10 ELISA plates.
Kit components: • Coating Buffer A (10 mM phosphate buffer, pH 7.4) • Coating Buffer B (50 mM carbonate buffer, pH 9.4) • Assay Buffer (Blocking Buffer) (5X) • Wash Buffer (25X) • Stabilized TMB • Stop Solution
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)ELISA
FormConcentrate
Shipping ConditionWet Ice
Quantity10 plates
Unit SizeEach
Contents & Storage
Coating Buffer A (100 mL): 1 bottle Coating Buffer B (100 mL): 1 bottle Assay Buffer (5x) (200 mL): 1 bottle Wash Buffer (25x) (100 mL): 3 bottles Stabilized Chromogen (25 mL): 4 bottles Stop Solution (100 mL): 1 bottle. Store at 2°C to 8°C.
Frequently asked questions (FAQs)
How do I develop a sandwich ELISA using Cytosets?
Each CytoSets contains capture (coating) antibody, biotinlyated detection antibody, standard and Streptavidin-HRP. Other reagents required are listed in the CytoSets information sheet included with the kit and can be purshased from us separately (Antibody Pair Buffer kit CNB0011, 5x Assay Buffer DS98200, etc.). The information sheet also provides a specific procedure and illustrates an example standard curve which can be obtained when the specific procedure is followed. A general procedure is summarized here:
1) Coat the microplate with diluted capture (coating) antibody overnight at 2-8 degrees C; Wash the plate
2) Incubate standards or samples with the coated microplate; Wash the plate
3) Incubate diluted biotinlyated detection antibody with the plate; Wash the plate
4) Incubate Streptavidin-HRP with the plate for 15-45 minutes; Wash the plate
5) Incubate the plate with TMB substrate for 10-60 minutes and stop the reaction with Stop solution
6) Read microplate at 450 nm.
Investigators are advised to determine optimal buffer formulations, concentrations and incubation times for individual applications.