PVDF/Filter Paper Sandwiches, 0.2 μm, have excellent binding properties for western blotting, dot-blot assays, and other protein or nucleic acid methods such as protein sequencing. The 0.2-μm pore size makes these membranes ideal for protein analysis of small amounts of proteins (as little as 10 pmoles), amino acid analysis, and transfer of small molecular weight proteins and peptides.

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Features
• Membrane: polyvinylidene difluoride (PVDF)
• Filter paper thickness: 0.8 mm
• Binding capacity: 50–150 μg/cm² for large, globular proteins; >150 μg/cm² for smaller peptides
• Re-probe characteristics: yes
• Pre-activation: required with 100% alcohol (methanol, ethanol, or isopropanol)
• Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling
• Durability: compatible with most organic solvents, acids, and mild bases