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CD3e Antibody (CD0329) in Flow
Human PBMCs stained with: CD45 Pacific Orange&##8482; (MHCD4530TR), CD4 PerCP-Cy&##174;5.5 (A15858), CD20 APC, CD14 APC-Cy&##174;7 (A15453), CD19 Pacific Green&##8482; (C11210), CD3 Alexa Fluor&##174; 700 (CD0329), HLA-DR PE-Cy&##174;7 (A18558), CD8 Pacific Blue&##8482; (MHCD0828), CD33 FITC (A16185), and CD11c PE (A18674) using the Attune&##174; NxT Acoustic Focusing Cytometer with 405 nm excitation and 440/50 emission filter (Pacific Blue&##8482;), 512/25 emission filter (Pacific Green&##8482;), and 603/48 emission filter (Pacific Orange&##8482;); 488 nm excitation and 530/30 emission filter (FITC) and 695/50 emission filter (PerCP-Cy&##174;5.5); 561 nm excitation and 585/16 emission filter (PE) and 780/60 emission filter (PE-Cy&##174;7); 637 nm excitation and 660/20 emission filter (APC), 720/30 emission filter (Alexa Fluor&##174;700), and 780/60 emission filter (APC-Cy&##174;7). Within the CD3 negative CD45 positive gate, B cells can be identified based on expression of CD19 and CD20. Conventional dendritic cells in peripheral blood are generally negative for T and B cell lineage markers and co-express the integrin CD11c and HLA-DR. Values noted are a percent of the parent CD19 negative CD20 negative gate (top value, no parenthesis) or percent of FSC/SSC lymphocyte gate (bottom value, in parentheses).
