Invitrogen

CD24 Monoclonal Antibody (30-F1), PE, eBioscience™

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Published Figures (9)

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CD24 Antibody (12-0241-82) in Flow

Stem cells (Dayton, Ohio) 2021 - 5 FIGURE Adipose stem cells (ASCs) are less numerous in ingWAT and BAT from in Lyl1 -/- mice, and Lyl1 is not expressed in the adipocyte lineage. A, Dot plots showing FACS analysis of SVFs isolated from ingWAT of a 6-week-old WT mouse and the gating strategy to isolate preadipocytes (CD140a+ CD24-, green) and ASCs (CD140a+ CD24+, blue). B, ASCs and preadipocytes were quantified by flow cytometry in SVFs iso... View More

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Product Details

12-0241-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2c, kappa

Class

Monoclonal

Type

Antibody

Clone

30-F1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465599

Product Specific Information

Description: The 30-F1 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. The expression of CD24 detected by 30-F1 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and different antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations.

Applications Reported: The 30-F1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 30-F1 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

In Lyl1^-/- mice, adipose stem cell vascular niche impairment leads to premature development of fat tissues.

Stem cells (Dayton, Ohio)

Hussain A,Deleuze V,El Kebriti L,Turali H,Pirot N,Glasson Y,Mathieu D,Pinet V

Published figure using CD24 monoclonal antibody (Product # 12-0241-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Cbl Ubiquitin Ligases Control B Cell Exit from the Germinal-Center Reaction.

Immunity

Li X,Gadzinsky A,Gong L,Tong H,Calderon V,Li Y,Kitamura D,Klein U,Langdon WY,Hou F,Zou YR,Gu H

12-0241-82 was used in Flow Cytometry to show that deleting E3 ubiquitin ligases Cbl and Cbl-b (Cbls) in GC B cells results in the early exit of high-affinity antigen-specific B cells from the GC reaction and thus impaired clonal expansion.

Tue Mar 20 00:00:00 EDT 2018

Gli3 in fetal thymic epithelial cells promotes thymocyte positive selection and differentiation by repression of Shh.

Development (Cambridge, England)

Solanki A,Yanez DC,Ross S,Lau CI,Papaioannou E,Li J,Saldaña JI,Crompton T

Published figure using CD24 monoclonal antibody (Product # 12-0241-82) in Flow Cytometry

Wed Jan 31 00:00:00 EST 2018

Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration.

Cell stem cell

Ambrosi TH,Scialdone A,Graja A,Gohlke S,Jank AM,Bocian C,Woelk L,Fan H,Logan DW,Schürmann A,Saraiva LR,Schulz TJ

12-0241 was used in Flow cytometry/Cell sorting to specify the cellular identities of the adipogenic and osteogenic lineages of the bone.

Thu Jun 01 00:00:00 EDT 2017

Breast cancer stem cells expressing different stem cell markers exhibit distinct biological characteristics.

Molecular medicine reports

Shao J,Fan W,Ma B,Wu Y

12-0241 was used in Flow cytometry/Cell sorting to demonstrate that breast cancer stem cells are heterogeneous, and they exhibit distinct biological characteristics.

Thu Dec 01 00:00:00 EST 2016

PRDM14 promotes RAG-dependent Notch1 driver mutations in mouse T-ALL.

Biology open

Carofino BL,Ayanga B,Tracey LJ,Brooke-Bisschop T,Justice MJ

12-0241 was used in Flow cytometry/Cell sorting to investigate how PRDM14 promotes RAG-dependent Notch1 driver mutations in mouse T-ALL.

Sun May 15 00:00:00 EDT 2016

Neuregulin autocrine signaling promotes self-renewal of breast tumor-initiating cells by triggering HER2/HER3 activation.

Cancer research

Lee CY,Lin Y,Bratman SV,Feng W,Kuo AH,Scheeren FA,Engreitz JM,Varma S,West RB,Diehn M

12-0241 was used in Flow cytometry/Cell sorting to demonstrate a mechanism through which HER2-targeting therapies could benefit a larger number of breast cancer patients.

Wed Jan 01 00:00:00 EST 2014

Notch2 genetic fate mapping reveals two previously unrecognized mammary epithelial lineages.

Nature cell biology

Šale S,Lafkas D,Artavanis-Tsakonas S

Published figure using CD24 monoclonal antibody (Product # 12-0241-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

12-0241 was used in Flow cytometry/Cell sorting to study how the GATA3-miR-29b axis regulates the tumour microenvironment, and inhibits metastasis to open up possibilities for therapeutic intervention in breast cancer.

Fri Feb 01 00:00:00 EST 2013

Leptin receptor maintains cancer stem-like properties in triple negative breast cancer cells.

Endocrine-related cancer

Zheng Q,Banaszak L,Fracci S,Basali D,Dunlap SM,Hursting SD,Rich JN,Hjlemeland AB,Vasanji A,Berger NA,Lathia JD,Reizes O

12-0241 was used in Flow cytometry/Cell sorting to explore the role of the leptin receptor in the maintenance of cancer stem cells.

Sun Dec 01 00:00:00 EST 2013

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

12-0241 was used in Flow cytometry/Cell sorting to demonstrate that increased expression of Prdm14 drives development of T-Cell acute lymphoblastic leukemia in mice.

Fri Nov 01 00:00:00 EDT 2013

Nuclear export of histone deacetylase 7 during thymic selection is required for immune self-tolerance.

The EMBO journal

Kasler HG,Lim HW,Mottet D,Collins AM,Lee IS,Verdin E

12-0241 was used in Flow cytometry/Cell sorting to examine the effect of blocking TCR-dependent nuclear export of HDAC7 during thymic selection, showing that HDAC7 is required for immune self-tolerance.

Wed Nov 28 00:00:00 EST 2012

VCAM-1 blockade delays disease onset, reduces disease severity and inflammatory cells in an atopic dermatitis model.

Immunology and cell biology

Chen L,Lin SX,Amin S,Overbergh L,Maggiolino G,Chan LS

12-0241 was used in Flow cytometry/Cell sorting to investigate the functions of ICAM-1 and VCAM-1 in a keratin-14 IL-4-Tg mouse model of atopic dermatitis.

Fri Jun 11 00:00:00 EDT 2010

Immune-induced epithelial to mesenchymal transition in vivo generates breast cancer stem cells.

Cancer research

Santisteban M,Reiman JM,Asiedu MK,Behrens MD,Nassar A,Kalli KR,Haluska P,Ingle JN,Hartmann LC,Manjili MH,Radisky DC,Ferrone S,Knutson KL

12-0241 was used in Flow cytometry/Cell sorting to show that epithelial to mesenchymal transition can produce the breast cancer stem cell phenotype.

Wed Apr 01 00:00:00 EDT 2009

TOX provides a link between calcineurin activation and CD8 lineage commitment.

The Journal of experimental medicine

Aliahmad P,O'Flaherty E,Han P,Goularte OD,Wilkinson B,Satake M,Molkentin JD,Kaye J

12-0241 was used in Flow cytometry/Cell sorting to demonstrate that up-regulation of TOX in double positive thymocytes is calcineurin dependent, linking the signalling pathway to nuclear changes that occur during positive thymocyte selection..

Mon Apr 19 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: CD24; cluster of differentiation 24; FLJ22950; FLJ43543; heat stable antigen; HSA; Ly-52; Lymphocyte antigen 52; M1/69-J11D heat stable antigen; MGC75043; Nectadrin; R13-Ag; Signal transducer CD24; X62 heat stable antigen

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Gene Aliases: Cd24; Cd24a; HSA; Ly-52; nectadrin

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UniProt ID: (Mouse) P24807

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Entrez Gene ID: (Mouse) 12484

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Function(s)

protein binding protein kinase binding carbohydrate binding protein tyrosine kinase activator activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD24 Monoclonal Antibody (30-F1), PE, eBioscience™

CD24 Antibody (12-0241-82) in Flow

Product Details

12-0241-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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