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Invitrogen

14-3-3 sigma Polyclonal Antibody

View all (34) 14-3-3 sigma antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (7)

Application

14-3-3 sigma Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

14-3-3 sigma Antibody (PA5-27147) in ICC/IF

14-3-3 sigma Polyclonal Antibody [C1C3] detects 14-3-3 sigma protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold Methanol for 5 min. Green: 14-3-3 sigma protein stained by 14-3-3 sigma Polyclonal Antibody [C1C3] (Product # PA5-27147) diluted at 1:500. Blue: Hoechst 33343 staining.

14-3-3 sigma Antibody in Immunohistochemistry (Paraffin) (IHC (P))

14-3-3 sigma Antibody in Western Blot (WB)

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Product Details

PA5-27147

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human 14-3-3 sigma. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2544623

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro2A, GL261, PC-12, A549.

Predicted reactivity: Mouse (100%), Pig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 14-3-3 protein sigma; 14-3-3 sigma; 14-3-3 sigma protein; 14-3-3sigma; Epithelial cell marker protein 1; HGNC:10773; Stratifin; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, sigma polypeptide

Gene Aliases: Er; HME1; Mkrn3; Mme1; SFN; YWHAS

UniProt ID: (Human) P31947, (Mouse) O70456

Entrez Gene ID: (Human) 2810, (Mouse) 55948, (Rat) 313017

Function(s)

protein binding protein kinase C inhibitor activity protein kinase binding protein domain specific binding phosphoprotein binding cadherin binding involved in cell-cell adhesion molecular_function scaffold/adaptor protein

Process(es)

regulation of cyclin-dependent protein serine/threonine kinase activity release of cytochrome c from mitochondria keratinocyte development negative regulation of protein kinase activity DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest signal transduction intrinsic apoptotic signaling pathway in response to DNA damage regulation of epidermal cell division negative regulation of keratinocyte proliferation positive regulation of cell growth keratinization negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of epidermal cell differentiation positive regulation of protein export from nucleus membrane organization establishment of skin barrier negative regulation of protein serine/threonine kinase activity cell-cell adhesion positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway keratinocyte differentiation skin development regulation of cell cycle biological_process

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